2018
Intravenous infusion of mesenchymal stem cells promotes functional recovery in a rat model of chronic cerebral infarction.
Namioka T, Namioka A, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells promotes functional recovery in a rat model of chronic cerebral infarction. Journal Of Neurosurgery 2018, 131: 1289-1296. PMID: 30485210, DOI: 10.3171/2018.5.jns18140.Peer-Reviewed Original ResearchMiddle cerebral artery occlusionCerebral infarctionIntravenous infusionMesenchymal stem cellsChronic phaseRat modelBone marrowBlood-brain barrier integrityCerebral artery occlusionVehicle-treated groupChronic cerebral infarctionPhase of strokeStem cellsAdult bone marrowArtery occlusionBBB leakageMotor recoveryFunctional recoveryIschemic volumeFunctional outcomeSystemic infusionBrain parenchymaFunctional improvementSystemic injectionClinical studiesIntravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat.
Namioka A, Namioka T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat. Journal Of Neurosurgery 2018, 131: 1308-1316. PMID: 30485204, DOI: 10.3171/2018.4.jns173121.Peer-Reviewed Original ResearchBasilar artery occlusionIschemic lesion volumeInfused mesenchymal stem cellsIntravenous infusionMesenchymal stem cellsLesion volumeBrainstem infarctionMSC infusionFunctional recoveryIntravenous MSC infusionIschemic brainstem lesionsPosterior circulation strokeAcute stroke therapyArtery occlusion modelGroup 28 daysNylon monofilament sutureReliable animal modelMultiple behavioral testsStem cellsProximal BAArtery occlusionBrainstem lesionsPosterior circulationStroke therapyFunctional outcomeFunctional recovery after the systemic administration of mesenchymal stem cells in a rat model of neonatal hypoxia-ischemia.
Sakai T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Fukumura S, Kobayashi M, Tsutsumi H, Kocsis JD, Honmou O. Functional recovery after the systemic administration of mesenchymal stem cells in a rat model of neonatal hypoxia-ischemia. Journal Of Neurosurgery Pediatrics 2018, 22: 513-522. PMID: 30074448, DOI: 10.3171/2018.5.peds1845.Peer-Reviewed Original ResearchConceptsNeonatal hypoxia-ischemiaMesenchymal stem cellsHypoxia-ischemiaRat modelHypoxic-ischemic encephalopathyStem cellsCerebral palsyFunctional recoveryFunctional outcomeSystemic administrationIntravenous administrationAnimal studiesBrain volumePotential treatmentAdministrationPalsyCellsPatientsEncephalopathySynaptogenesis
2016
Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats
Sasaki Y, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Suzuki J, Tateyama D, Oka S, Namioka T, Namioka A, Onodera R, Mikami T, Wanibuchi M, Kakizawa M, Ishiai S, Kocsis JD, Honmou O. Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats. Physical Therapy 2016, 96: 1791-1798. PMID: 27174259, DOI: 10.2522/ptj.20150504.Peer-Reviewed Original ResearchConceptsMiddle cerebral artery occlusionIntravenous infusionDaily rehabilitationMesenchymal stem cellsMSC infusionFunctional outcomePermanent middle cerebral artery occlusionLimb placement testCerebral artery occlusionRat stroke modelRat MCAO modelIntraluminal vascular occlusionMagnetic resonance imagingStem cellsAdult bone marrowArtery occlusionCerebral ischemiaMCAO modelVascular occlusionFunctional improvementLesion volumeTherapy groupTherapeutic effectStroke modelGroup 2
2014
Possible neural plasticity detected by fMRI associates with improved motor function following intravenous injection of mesenchymal stem cells in a rat stroke model
Nakazaki M, Suzuki J, Sasaki M, Oka S, Sasaki Y, Honmou O. Possible neural plasticity detected by fMRI associates with improved motor function following intravenous injection of mesenchymal stem cells in a rat stroke model. Cerebral Blood Flow And Metabolism (Japanese Journal Of Cerebral Blood Flow And Metabolism) 2014, 25: 67. DOI: 10.16977/cbfm.25.2_67.Peer-Reviewed Original ResearchFunctional recoveryMesenchymal stem cellsLesion volumeSensorimotor cortexMotor functionInfused groupIschemic stroke model ratsStroke model ratsGreater functional recoveryImproved motor functionImproved functional outcomesAutologous mesenchymal stem cellsRat stroke modelRandomized-clinical trialFMRI patternsStem cellsCerebral infarctionStroke patientsFunctional outcomeIntravenous transplantationModel ratsMSCs groupFunctional deficitsSomatosensory cortexClinical trials
2012
Bilateral cortical hyperactivity detected by fMRI associates with improved motor function following intravenous infusion of mesenchymal stem cells in a rat stroke model
Suzuki J, Sasaki M, Harada K, Bando M, Kataoka Y, Onodera R, Mikami T, Wanibuchi M, Mikuni N, Kocsis JD, Honmou O. Bilateral cortical hyperactivity detected by fMRI associates with improved motor function following intravenous infusion of mesenchymal stem cells in a rat stroke model. Brain Research 2012, 1497: 15-22. PMID: 23274536, DOI: 10.1016/j.brainres.2012.12.028.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain InfarctionCerebral CortexDisease Models, AnimalExercise TestImage Processing, Computer-AssistedInfarction, Middle Cerebral ArteryMagnetic Resonance ImagingMesenchymal Stem Cell TransplantationMesenchymal Stem CellsMovement DisordersOxygenRatsRats, Sprague-DawleyStatistics, NonparametricTime FactorsConceptsMSC groupMesenchymal stem cellsLesion volumeFunctional MRISensorimotor cortexMotor functionElectrical stimulationRat cerebral infarction modelGreater functional recoveryCerebral infarction modelImproved motor functionImproved functional outcomesRat stroke modelHigh-intensity signalStem cellsFunctional recoveryBilateral signalsCortical hyperactivityFunctional outcomeIntravenous infusionIntravenous transplantationFunctional deficitsSomatosensory cortexInfused groupStroke model
2009
BDNF-Hypersecreting Human Mesenchymal Stem Cells Promote Functional Recovery, Axonal Sprouting, and Protection of Corticospinal Neurons after Spinal Cord Injury
Sasaki M, Radtke C, Tan AM, Zhao P, Hamada H, Houkin K, Honmou O, Kocsis JD. BDNF-Hypersecreting Human Mesenchymal Stem Cells Promote Functional Recovery, Axonal Sprouting, and Protection of Corticospinal Neurons after Spinal Cord Injury. Journal Of Neuroscience 2009, 29: 14932-14941. PMID: 19940189, PMCID: PMC2825276, DOI: 10.1523/jneurosci.2769-09.2009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain-Derived Neurotrophic FactorCells, CulturedCytoprotectionDisease Models, AnimalFemaleGene ExpressionGenetic VectorsGrowth ConesHumansMesenchymal Stem Cell TransplantationNerve RegenerationNeuronal PlasticityPyramidal TractsRatsRats, Sprague-DawleyRecovery of FunctionSpinal Cord InjuriesTransfectionTransplantation, HeterologousTreatment OutcomeConceptsSpinal cord injuryMesenchymal stem cellsCord injuryFunctional outcomeBone marrowAcute spinal cord injuryBrain-derived neurotrophic factorCorticospinal tract neuronsNumber of FGImproved functional outcomesPrimary motor cortexSpinal gray matterPotential therapeutic effectsStem cellsM1 cortexTransection lesionCorticospinal neuronsTract neuronsAxonal sproutingFunctional recoveryVentral hornNeuronal densitySerotonergic fibersLesion cavityMotor cortexUnique in vivo properties of olfactory ensheathing cells that may contribute to neural repair and protection following spinal cord injury
Kocsis JD, Lankford KL, Sasaki M, Radtke C. Unique in vivo properties of olfactory ensheathing cells that may contribute to neural repair and protection following spinal cord injury. Neuroscience Letters 2009, 456: 137-142. PMID: 19429149, PMCID: PMC2713444, DOI: 10.1016/j.neulet.2008.08.093.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCord injuryLow-affinity NGF receptorSchwann cell transplantationSignificant functional improvementSpinal cord resultsOlfactory receptor axonsPrecise cellular mechanismsCord resultsCell transplantationFunctional outcomeSynaptic contactsAxonal regenerationNasal mucosaFunctional improvementSpinal cordGlial cellsOlfactory bulbReceptor axonsTrophic supportNGF receptorAnimal modelsNeural repairTherapeutic candidateOECs
2008
Potential of olfactory ensheathing cells for cell-based therapy in spinal cord injury
Radtke C, Sasaki M, Lankford KL, Vogt PM, Kocsis JD. Potential of olfactory ensheathing cells for cell-based therapy in spinal cord injury. The Journal Of Rehabilitation Research And Development 2008, 45: 141-152. PMID: 18566933, DOI: 10.1682/jrrd.2007.03.0049.Peer-Reviewed Original ResearchConceptsSpinal cord injuryPeripheral nervous systemCentral nervous systemCord injuryAxonal regenerationNervous systemTreatment of SCIContusive spinal cord injuryPotential of olfactoryPermanent neurological deficitsSpecialized glial cellsCell therapy approachesCell transplantation approachesOEC biologyAxonal lossNeurological deficitsCell-based therapiesFunctional outcomeGlial cellsClinical studiesComplex lesionsMedicine cliniciansMacrophage activationTransplantation approachesInhibitory molecules
2007
Demyelinating diseases and potential repair strategies
Radtke C, Spies M, Sasaki M, Vogt PM, Kocsis JD. Demyelinating diseases and potential repair strategies. International Journal Of Developmental Neuroscience 2007, 25: 149-153. PMID: 17408905, PMCID: PMC2692731, DOI: 10.1016/j.ijdevneu.2007.02.002.Peer-Reviewed Original ResearchConceptsMultiple sclerosisInjury modelSpinal cord injuryCell-based strategiesAxon lossNerve compressionNeuroprotective potentialCord injuryFunctional outcomeClinical studiesMS lesionsTherapeutic goalsVulnerable axonsCellular transplantationNeurological disordersDemyelinationRemyelinationNeuroprotectionPotential repair strategiesCell typesSclerosisTransplantationInjuryLesionsAxonsRemyelination of the injured spinal cord
Sasaki M, Li B, Lankford KL, Radtke C, Kocsis JD. Remyelination of the injured spinal cord. Progress In Brain Research 2007, 161: 419-433. PMID: 17618995, PMCID: PMC2605400, DOI: 10.1016/s0079-6123(06)61030-3.Peer-Reviewed Original ResearchConceptsSpinal cord injuryFunctional outcomeSpinal cordContusive spinal cord injuryLong white matter tractsPotential cell therapy candidatesWhite matter tractsMyelin-forming cellsCell therapy candidatesCord injuryCentral necrotic coreClinical studiesTherapy candidatesNecrotic coreTransplantation studiesExperimental modelCordOutcomesCellsDemyelinationRemyelinationInjuryNecrosisOECsAxons
2006
Myelination and nodal formation of regenerated peripheral nerve fibers following transplantation of acutely prepared olfactory ensheathing cells
Dombrowski MA, Sasaki M, Lankford KL, Kocsis JD, Radtke C. Myelination and nodal formation of regenerated peripheral nerve fibers following transplantation of acutely prepared olfactory ensheathing cells. Brain Research 2006, 1125: 1-8. PMID: 17112480, PMCID: PMC2673087, DOI: 10.1016/j.brainres.2006.09.089.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell Adhesion Molecules, NeuronalCell TransplantationGreen Fluorescent ProteinsImmunohistochemistryMicroscopy, ImmunoelectronMyelin SheathNAV1.6 Voltage-Gated Sodium ChannelNerve RegenerationNeurofilament ProteinsNeurogliaOlfactory BulbRanvier's NodesRatsRats, Sprague-DawleySciatic NeuropathySodium ChannelsTime FactorsConceptsPeripheral nerve fibersPeripheral nervesNodes of RanvierFunctional outcomeAxonal regenerationNerve fibersRegenerated peripheral nerve fibersSciatic nerve crush lesionNerve crush lesionPeripheral-type myelinSpinal cord resultsTransplantation of olfactoryPeripheral axonal regenerationParanodal CasprCrush lesionCord resultsFunctional improvementOlfactory bulbTransection siteTransgenic ratsLesion zoneNerveNodal formationTransplantation siteOECs
2005
Protection of corticospinal tract neurons after dorsal spinal cord transection and engraftment of olfactory ensheathing cells
Sasaki M, Hains BC, Lankford KL, Waxman SG, Kocsis JD. Protection of corticospinal tract neurons after dorsal spinal cord transection and engraftment of olfactory ensheathing cells. Glia 2005, 53: 352-359. PMID: 16288464, PMCID: PMC2605395, DOI: 10.1002/glia.20285.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzimidazolesCell CountCell SeparationCell SurvivalCell TransplantationEnzyme-Linked Immunosorbent AssayFluorescent DyesImage Processing, Computer-AssistedImmunohistochemistryIn Situ Nick-End LabelingMotor ActivityNeuronsOlfactory PathwaysPyramidal TractsRatsSpinal Cord InjuriesStilbamidinesConceptsCorticospinal tract neuronsSpinal cordTract neuronsNeuronal lossOEC transplantationDorsal spinal cord transectionPrimary motor cortexCortical projection neuronsSpinal cord transectionTransplantation of olfactoryRat spinal cordOlfactory Ensheathing CellsApoptotic cortical neuronsBDNF levelsNeuroprotective effectsCord transectionFunctional outcomeMotor cortexNeurotrophic moleculesProjection neuronsAxonal regenerationCortical neuronsInjury zoneTransplantationOECs28 Transplantation of Peripheral-Myelin-Forming Cells to Repair Demyelinated Axons
Kocsis J, Sasaki M. 28 Transplantation of Peripheral-Myelin-Forming Cells to Repair Demyelinated Axons. 2005, 421-433. DOI: 10.1016/b978-012738761-1/50029-8.Peer-Reviewed Original ResearchTransplantation of SCsTransplantation of OECsSpinal cord injuryCentral nervous systemSchwann cellsAxonal regenerationBone marrow cellsFunctional recoveryMultiple sclerosisCell transplantationCord injurySpinal cordHind limb locomotor functionContusive spinal cord injuryBone marrow cell transplantationMarrow cellsSpinal cord injury modelMarrow cell transplantationCerebral ischemia modelSpinal cord resultsGlobal neuroprotectionDemyelination modelCord resultsFunctional outcomeDemyelinated axons