2021
Repeated infusion of mesenchymal stem cells maintain the condition to inhibit deteriorated motor function, leading to an extended lifespan in the SOD1G93A rat model of amyotrophic lateral sclerosis
Magota H, Sasaki M, Kataoka-Sasaki Y, Oka S, Ukai R, Kiyose R, Onodera R, Kocsis JD, Honmou O. Repeated infusion of mesenchymal stem cells maintain the condition to inhibit deteriorated motor function, leading to an extended lifespan in the SOD1G93A rat model of amyotrophic lateral sclerosis. Molecular Brain 2021, 14: 76. PMID: 33962678, PMCID: PMC8103621, DOI: 10.1186/s13041-021-00787-6.Peer-Reviewed Original ResearchConceptsAmyotrophic lateral sclerosisAdministration of MSCsSingle infusionMesenchymal stem cellsMotor functionGait abilityDisease progressionMotor neuronsRat modelLateral sclerosisBlood-spinal cord barrierSOD1G93A rat modelSpinal cord degenerateVehicle infusion groupHind limb functionALS rat modelStem cellsLimb functionVehicle infusionFatal disorderSurvival periodTherapeutic effectLocomotor activityInfusionProgressive deteriorationIntravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model
Magota H, Sasaki M, Kataoka-Sasaki Y, Oka S, Ukai R, Kiyose R, Onodera R, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model. Brain Research 2021, 1757: 147296. PMID: 33516815, DOI: 10.1016/j.brainres.2021.147296.Peer-Reviewed Original ResearchConceptsBlood-spinal cord barrierQuantitative reverse transcription polymerase chain reactionIntravenous infusionDisease progressionMotor neuronsMSC groupLocomotor functionOpen-field locomotor functionPreservation of microvasculatureHind limb functionCommon clinical featuresEvans blue leakageMotor neuron lossReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionDevastating neurodegenerative diseaseBBB scoringBSCB functionRotarod testingClinical featuresNeuron lossLimb functionNeurotrophic factorCurative strategiesSpinal cord
2018
Intravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury
Oshigiri T, Sasaki T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Oka S, Morita T, Hirota R, Yoshimoto M, Yamashita T, Hashimoto-Torii K, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury. Journal Of Neurotrauma 2018, 36: 411-420. PMID: 29901416, PMCID: PMC6352512, DOI: 10.1089/neu.2018.5793.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfused mesenchymal stem cellsMesenchymal stem cellsCord injuryIntravenous infusionFunctional improvementAcute spinal cord injuryBlood-spinal cord barrierGene expression signaturesAxonal sproutingFunctional recoveryMotor cortexSystemic infusionVehicle infusionSpinal cordRat modelTherapeutic mechanismImproved functionInfusionTranscription-polymerase chain reaction dataPearson correlation analysisBehavioral functionsExpression signaturesGenome-wide RNA profilingRecent evidence
2015
Diffuse and persistent blood–spinal cord barrier disruption after contusive spinal cord injury rapidly recovers following intravenous infusion of bone marrow mesenchymal stem cells
Matsushita T, Lankford KL, Arroyo EJ, Sasaki M, Neyazi M, Radtke C, Kocsis JD. Diffuse and persistent blood–spinal cord barrier disruption after contusive spinal cord injury rapidly recovers following intravenous infusion of bone marrow mesenchymal stem cells. Experimental Neurology 2015, 267: 152-164. PMID: 25771801, DOI: 10.1016/j.expneurol.2015.03.001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, SurfaceBlood-Brain BarrierCell- and Tissue-Based TherapyDisease Models, AnimalEndothelial CellsExploratory BehaviorGlial Fibrillary Acidic ProteinLocomotionMaleMesenchymal Stem CellsMicrovesselsPermeabilityRatsRats, Sprague-DawleyRats, TransgenicReceptor, Platelet-Derived Growth Factor betaSpinal Cord InjuriesTime FactorsVon Willebrand FactorConceptsSpinal cord injuryContusive spinal cord injuryBlood-spinal cord barrierBSCB leakageIntravenous infusionMesenchymal stem cellsVon Willebrand factorMSC infusionCord injurySpinal cordBlood-spinal cord barrier disruptionExperimental spinal cord injuryIntravenous MSC infusionSpinal cord barrierEx vivo optical imagingDissociation of pericytesBone marrow mesenchymal stem cellsStem cellsMarrow mesenchymal stem cellsBSCB integrityBSCB permeabilityLocomotor recoveryPost-SCIBarrier disruptionAntigen expression