2023
Emergence of Extended-Spectrum Cephalosporin- and Colistin-Resistant Enterobacterales in Otherwise Healthy University Students
Osman M, Yassine I, Hamze M, Al Mir H, Tajani A, Bisha B, Cummings K, Madec J, Haenni M, Kassem I. Emergence of Extended-Spectrum Cephalosporin- and Colistin-Resistant Enterobacterales in Otherwise Healthy University Students. Microbial Drug Resistance 2023, 30: 101-107. PMID: 38011748, DOI: 10.1089/mdr.2023.0213.Peer-Reviewed Original ResearchLast-resort antibioticsHealthy university studentsExtended-spectrum cephalosporin-resistant isolatesComprehensive antimicrobial stewardship programAntimicrobial stewardship programsColistin-resistant isolatesExtended-spectrum cephalosporinsCephalosporin-resistant isolatesColistin-resistant EnterobacteralesMultidrug-resistant bacteriaMiddle-income countriesEvidence-based practiceAntimicrobial stewardshipStewardship programsOccurrence of resistanceCommunity settingsAntimicrobial resistanceClinical samplesAntibiotic resistanceFecal samplesResistance determinantsAntibioticsPublic healthMultiple factorsIsolates
2022
Analysis of pCl107 a large plasmid carried by an ST25 Acinetobacter baumannii strain reveals a complex evolutionary history and links to multiple antibiotic resistance and metabolic pathways
Rafei R, Koong J, Osman M, Al Atrouni A, Hamze M, Hamidian M. Analysis of pCl107 a large plasmid carried by an ST25 Acinetobacter baumannii strain reveals a complex evolutionary history and links to multiple antibiotic resistance and metabolic pathways. FEMS Microbes 2022, 3: xtac027. PMID: 37332503, PMCID: PMC10117892, DOI: 10.1093/femsmc/xtac027.Peer-Reviewed Original ResearchComplex evolutionary historyEvolutionary historyMetabolic pathwaysHybrid assembly approachConjugative transfer systemOxford Nanopore sequencingLarge plasmidsMetabolic modulesComplete sequenceMetabolism modulesAntibiotic resistance genesAncestral structureIllumina MiSeqNanopore sequencingResistance genesPossible ancestorAntibiotic resistanceGlobal clone 2PlasmidMultiple antibiotic resistanceClone 2Important pathogenResistance islandsKb plasmidSequence types
2021
Cl415, a carbapenem-resistant Acinetobacter baumannii isolate containing four AbaR4 and a new variant of AbGRI2, represents a novel global clone 2 strain
Liepa R, Mann R, Osman M, Hamze M, Gunawan C, Hamidian M. Cl415, a carbapenem-resistant Acinetobacter baumannii isolate containing four AbaR4 and a new variant of AbGRI2, represents a novel global clone 2 strain. Journal Of Antimicrobial Chemotherapy 2021, 77: 345-350. PMID: 34741594, PMCID: PMC8809195, DOI: 10.1093/jac/dkab399.Peer-Reviewed Original ResearchConceptsWhole genome sequencesWide geographical distributionGene complementNovel variantsPhylogenetic analysisGenomic analysisNew lineageComplete genomeOxford NanoporeIllumina MiSeqGenetic contextResistance genesGenesAntibiotic resistance determinantsResistance phenotypeGeographical distributionCopiesAntibiotic resistance phenotypesResistance islandsGlobal distributionResistance determinantsComM geneAntibiotic resistanceSubcladesVariants
2019
Molecular epidemiology of Campylobacter isolates from broiler slaughterhouses in Tripoli, North of Lebanon
Rafei R, Kassaa I, Osman M, Dabboussi F, Hamze M. Molecular epidemiology of Campylobacter isolates from broiler slaughterhouses in Tripoli, North of Lebanon. British Poultry Science 2019, 60: 675-682. PMID: 31321996, DOI: 10.1080/00071668.2019.1645945.Peer-Reviewed Original ResearchMeSH KeywordsAbattoirsAnimalsAnti-Bacterial AgentsCampylobacterCampylobacter InfectionsCecumChickensDrug Resistance, BacterialElectrophoresis, Gel, Pulsed-FieldFlagellinFoodborne DiseasesGenetic VariationHumansLebanonPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPoultry DiseasesPrevalenceSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationConceptsAntibiotic resistance profilesErtapenem resistanceMolecular epidemiologyReal burdenResistance profilesAntibiotic resistanceRestriction fragment length polymorphismNorth of LebanonFragment length polymorphismEpidemiologyField gel electrophoresisCampylobacterLength polymorphismBroiler slaughterhousesBroilersIsolatesSlaughterhouseGentamicinAmoxicillinErythromycin
2018
First Lebanese Antibiotic Awareness Week campaign: knowledge, attitudes and practices towards antibiotics
Al Omari S, Al Mir H, Wrayde S, Merhabi S, Dhaybi I, Jamal S, Chahine M, Bayaa R, Tourba F, Tantawi H, Al Atrouni A, Omari K, Ayash O, Zeidan N, Mallat H, Dabboussi F, Hamze M, Osman M. First Lebanese Antibiotic Awareness Week campaign: knowledge, attitudes and practices towards antibiotics. Journal Of Hospital Infection 2018, 101: 475-479. PMID: 30012377, DOI: 10.1016/j.jhin.2018.07.009.Peer-Reviewed Original Research
2016
Alarming increase in prevalence of Neisseria gonorrhoeae infections associated with a high level of antibiotic resistance in Tripoli, Lebanon
Hamze M, Osman M, Achkar M, Mallat H, Dabboussi F. Alarming increase in prevalence of Neisseria gonorrhoeae infections associated with a high level of antibiotic resistance in Tripoli, Lebanon. International Journal Of Antimicrobial Agents 2016, 48: 576-577. PMID: 27667565, DOI: 10.1016/j.ijantimicag.2016.08.003.Peer-Reviewed Original Research