2020
Actin flow-dependent and -independent force transmission through integrins
Driscoll TP, Ahn SJ, Huang B, Kumar A, Schwartz MA. Actin flow-dependent and -independent force transmission through integrins. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 32413-32422. PMID: 33262280, PMCID: PMC7768777, DOI: 10.1073/pnas.2010292117.Peer-Reviewed Original ResearchConceptsActin binding siteProtein interactionsDistinct protein interactionsDynamic protein interactionsIntegrin-dependent adhesionBinding sitesSubstrate stiffnessActin flowActin filamentsStiff substratesExtracellular matrixTalinVinculinIntegrinsReciprocal exchangeClutchesForce transmissionAdhesionFlow-independent mechanismsLarge adhesionABS3Cell edgeABS2SitesInteraction
2018
Vinculin and the mechanical response of adherent fibroblasts to matrix deformation
Rosowski KA, Boltyanskiy R, Xiang Y, Van den Dries K, Schwartz MA, Dufresne ER. Vinculin and the mechanical response of adherent fibroblasts to matrix deformation. Scientific Reports 2018, 8: 17967. PMID: 30568231, PMCID: PMC6299284, DOI: 10.1038/s41598-018-36272-9.Peer-Reviewed Original ResearchConceptsFocal adhesion protein vinculinAdhesion protein vinculinExtracellular matrix stiffnessIntegrin-mediated adhesionTraction forceProtein vinculinMechanical cuesCellular forcesMatrix stiffnessAdherent fibroblastsECM deformationMouse fibroblastsTraction stressMatrix deformationMechanical responseVinculinMechanical energyApparent stiffnessDeformationFibroblastsStiffnessCellsStretchForceExpression
2016
Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity
Kumar A, Ouyang M, Van den Dries K, McGhee EJ, Tanaka K, Anderson MD, Groisman A, Goult BT, Anderson KI, Schwartz MA. Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity. Journal Of Cell Biology 2016, 213: 371-383. PMID: 27161398, PMCID: PMC4862330, DOI: 10.1083/jcb.201510012.Peer-Reviewed Original ResearchConceptsTalin tension sensorFar C-terminusIntegrin-dependent adhesionPeripheral adhesionsTension sensorFocal adhesionsCellular mechanosensingMechanosensitive structuresTalin functionC-terminusDistinct functionsRod domainStiffness sensingTalinVinculinActin filamentsABS3Site 2Soft substratesAdhesionForce transmissionMechanosensingNew lightTerminus
2014
Tension-Sensitive Actin Assembly Supports Contractility at the Epithelial Zonula Adherens
Leerberg JM, Gomez GA, Verma S, Moussa EJ, Wu SK, Priya R, Hoffman BD, Grashoff C, Schwartz MA, Yap AS. Tension-Sensitive Actin Assembly Supports Contractility at the Epithelial Zonula Adherens. Current Biology 2014, 24: 1689-1699. PMID: 25065757, PMCID: PMC5103636, DOI: 10.1016/j.cub.2014.06.028.Peer-Reviewed Original ResearchConceptsMena/VASPActin assemblyEpithelial zonula adherensZonula adherensMyosin II activationActin filament turnoverCadherin junctionsActomyosin apparatusVASP proteinsActin scaffoldFilament turnoverActin filamentsJunctional integrityVinculinPhysical interactionTissue integrityActomyosin systemEpithelial cellsAdherensAssemblyContractile apparatusRegulationVASPEffective contractilityUnderlying mechanism
2010
Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature 2010, 466: 263-266. PMID: 20613844, PMCID: PMC2901888, DOI: 10.1038/nature09198.Peer-Reviewed Original ResearchConceptsFocal adhesionsFocal adhesion dynamicsMembrane cytoskeletal proteinsAdhesion dynamicsCell adhesion moleculeRegulatory mechanismsSpecific proteinsActin filamentsCell adhesionVinculinProteinMechanical tensionMechanical forcesRegulationPhysical forcesMolecular forcesAdhesionCellsVivoMechanotransductionPhysiologyNew biosensorFilamentsAbilityMigration