2018
Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors
Lopez Sambrooks C, Baro M, Quijano A, Narayan A, Cui W, Greninger P, Egan R, Patel A, Benes CH, Saltzman WM, Contessa JN. Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors. Cancer Research 2018, 78: canres.0505.2018. PMID: 30026325, PMCID: PMC6125176, DOI: 10.1158/0008-5472.can-18-0505.Peer-Reviewed Original ResearchConceptsMutant NSCLCMutant non-small cell lung cancerNon-small cell lung cancerSignificant tumor growth delayEGFR-TKI treatmentCell lung cancerTyrosine kinase inhibitor resistanceEGFR tyrosine kinase inhibitor resistanceLung cancer cell linesNGI-1Tumor growth delayEffective therapeutic targetCell linesKinase inhibitor resistanceTumor cell viabilityH1975 xenograftsCancer cell linesTKI treatmentComplex transmembrane proteinsEGFR-TKILung cancerTumor responseCell cycle arrestPreclinical modelsTherapeutic strategies
2012
Development and characterization of an isogenic cell line with a radioresistant phenotype
de Llobet L, Baro M, Figueras A, Modolell I, Da Silva M, Muñoz P, Navarro A, Mesia R, Balart J. Development and characterization of an isogenic cell line with a radioresistant phenotype. Clinical And Translational Oncology 2012, 15: 189-197. PMID: 22855182, DOI: 10.1007/s12094-012-0898-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBlotting, WesternCarcinoma, Squamous CellCell CycleCell MovementCell ProliferationDose-Response Relationship, RadiationFemaleFlow CytometryGamma RaysHumansHyaluronan ReceptorsMiceMice, NudePhenotypeRadiation ToleranceTumor Cells, CulturedVascular Endothelial Growth Factor AWound HealingXenograft Model Antitumor AssaysConceptsCell linesIsogenic cancer cell linesIsogenic cell linesRadiation-induced DNA fragmentationCancer cellsSpecific cell functionsDNA repair abilityBiological traitsAppropriate study modelsCell cycle distributionVascular endothelial growth factor (VEGF) secretionMolecular characterizationCell survivalDNA fragmentationRadioresistant phenotypeCell growthCancer cell linesSecretion of VEGFGrowth factor secretionSingle cellsCycle distributionCell functionRepair abilityRadiation resistanceWestern blot
2011
The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis
Balart J, Pueyo G, de Llobet L, Baro M, Sole X, Marin S, Casanovas O, Mesia R, Capella G. The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis. Radiation Oncology 2011, 6: 6. PMID: 21235815, PMCID: PMC3025872, DOI: 10.1186/1748-717x-6-6.Peer-Reviewed Original ResearchConceptsRadiation-induced DNA repairDNA repairCaspase inhibitorsDNA double-strand break repairChemical caspase inhibitorsSpontaneous DNA breakageDNA fragmentationDouble-strand break repairInterference of apoptosisSpecific caspase inhibitorsPulsed-field gel electrophoresisSpontaneous DNA fragmentationGel electrophoresisCell fateBreak repairDNA fragmentsApoptosis inhibitorH2AX activationAgarose plugsDNA breakageApoptosis inhibitionFunctional assaysCaspase-3ApoptosisCell cultures