2020
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
Vogels CBF, Brito AF, Wyllie AL, Fauver JR, Ott IM, Kalinich CC, Petrone ME, Casanovas-Massana A, Catherine Muenker M, Moore AJ, Klein J, Lu P, Lu-Culligan A, Jiang X, Kim DJ, Kudo E, Mao T, Moriyama M, Oh JE, Park A, Silva J, Song E, Takahashi T, Taura M, Tokuyama M, Venkataraman A, Weizman OE, Wong P, Yang Y, Cheemarla NR, White EB, Lapidus S, Earnest R, Geng B, Vijayakumar P, Odio C, Fournier J, Bermejo S, Farhadian S, Dela Cruz CS, Iwasaki A, Ko AI, Landry ML, Foxman EF, Grubaugh ND. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets. Nature Microbiology 2020, 5: 1299-1305. PMID: 32651556, PMCID: PMC9241364, DOI: 10.1038/s41564-020-0761-6.Peer-Reviewed Original ResearchConceptsSARS-CoV-2SARS-CoV-2 RTSevere acute respiratory syndrome coronavirusAcute respiratory syndrome coronavirusViral RNA copiesPublic health laboratoriesPublic health interventionsReverse transcription-PCR assaySARS-CoV-2 diagnostic testingDiagnostic assaysTranscription-PCR assaySARS-CoV-2 evolutionQuantitative reverse transcription-PCR assaysRapid diagnostic assaysHealth laboratoriesHealth interventionsDiagnostic testingRNA copiesPrimer-probe setsAssaysLow sensitivityCritical needAnalytical sensitivity
2008
False negative PCR despite high levels of JC virus DNA in spinal fluid: Implications for diagnostic testing
Landry ML, Eid T, Bannykh S, Major E. False negative PCR despite high levels of JC virus DNA in spinal fluid: Implications for diagnostic testing. Journal Of Clinical Virology 2008, 43: 247-249. PMID: 18701345, PMCID: PMC2683978, DOI: 10.1016/j.jcv.2008.07.003.Peer-Reviewed Original ResearchConceptsProgressive multifocal leukoencephalopathyPolymerase chain reactionHerpes simplex virus polymerase chain reactionSpinal fluidCSF polymerase chain reactionNegative polymerase chain reactionFatal neurologic illnessesJC virus DNAFalse-negative polymerase chain reactionHigher positive resultsMultifocal leukoencephalopathyBrain biopsyNeurologic illnessDNA polymerase chain reactionNeurologic diseaseBlinded studyPCR testingDiagnostic testingVirus DNAReference laboratoryYoung mothersChain reactionNegative resultsDifferent virusesPositive results