2006
Engraftment of Donor‐Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice
Bruscia EM, Ziegler EC, Price JE, Weiner S, Egan ME, Krause DS. Engraftment of Donor‐Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice. Stem Cells 2006, 24: 2299-2308. PMID: 16794262, DOI: 10.1634/stemcells.2006-0166.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBone Marrow TransplantationCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsFemaleFluorescent Antibody TechniqueHematopoietic Stem Cell TransplantationIn Situ Hybridization, FluorescenceMaleMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicRNA, MessengerY ChromosomeConceptsBone marrow-derived cellsMarrow-derived epithelial cellsBone marrow transplantationNewborn miceEpithelial cellsMarrow transplantationGI tractBone marrow-derived epithelial cellsDonor-derived epithelial cellsDoses of busulfanMarrow-derived cellsEngraftment of donorIrradiated adult recipientsMyeloablative regimenPreparative regimenAdult recipientsDifferent regimensEngrafted miceHematopoietic engraftmentGastrointestinal tractSurvival advantageTherapeutic benefitAdult miceMultiple organsBone marrow
1996
Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line.
Kearns W, Afione S, Fulmer S, Pang M, Erikson D, Egan M, Landrum M, Flotte T, Cutting G. Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line. Gene Therapy 1996, 3: 748-55. PMID: 8875221.Peer-Reviewed Original ResearchConceptsIB3-1 cellsWild-type AAVEpithelial cell lineMetaphase spreadsSite-specific fashionCFTR cDNACell linesLow molecular weight DNA fractionCF bronchial epithelial cell lineHuman chromosome 19Immortalized epithelial cell lineWeight DNA fractionMetaphase chromosome preparationsMetaphase chromosome spreadsBronchial epithelial cell lineDifferent chromosomesAAV-CFTR vectorCDNA integrationChromosome spreadsChromosome 19Genomic DNASite-specific mannerDNA fractionChromosome preparationsCFTR expression