1994
Functional expression and characterization of human D2 and D3 dopamine receptors
Potenza M, Graminski G, Schmauss C, Lerner. Functional expression and characterization of human D2 and D3 dopamine receptors. Journal Of Neuroscience 1994, 14: 1463-1476. PMID: 7907363, PMCID: PMC6577580, DOI: 10.1523/jneurosci.14-03-01463.1994.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological AssayCyclic AMPDopamine AgentsDopamine AntagonistsHumansLigandsMelanocyte-Stimulating HormonesMelanophoresMelatoninPertussis ToxinPigments, BiologicalReceptors, DopamineReceptors, Dopamine D2Receptors, Dopamine D3TetrahydronaphthalenesVirulence Factors, BordetellaXenopus laevisConceptsD3 receptorsCAMP accumulationGi protein-coupled receptorHuman D2Selective dopaminergic agonistsDose-dependent mannerIC50 value determinationDopaminergic agonistsMelanocyte receptorsPertussis toxinD3 dopamineAgonistsReceptorsPigment granule aggregationAntagonistD2RXenopus laevis melanophoresSimilar concentrationsFunctional expressionPigment aggregationD3R.Initial feasibility studyFunctional characteristicsCultured Xenopus laevis melanophoresNew bioassay
1992
A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay
Potenza M, Graminski G, Lerner M. A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay. Analytical Biochemistry 1992, 206: 315-322. PMID: 1332547, DOI: 10.1016/0003-2697(92)90372-e.Peer-Reviewed Original ResearchConceptsBeta-2 adrenergic receptorsAdrenergic receptorsGs protein-coupled receptorsEffects of drugsDose-response curveHuman beta 2 adrenergic receptorRank order potencyProtein-coupled receptorsReproducible dose-response curvesPharmacological characterizationConcomitant elevationStimulatory guaninePigment dispersionAgonistsIntracellular cAMPImportant receptorReceptorsAntagonistSpecific GsIC50 valuesFunctional expressionCellsXenopus laevis