A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay
Potenza M, Graminski G, Lerner M. A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay. Analytical Biochemistry 1992, 206: 315-322. PMID: 1332547, DOI: 10.1016/0003-2697(92)90372-e.Peer-Reviewed Original ResearchConceptsBeta-2 adrenergic receptorsAdrenergic receptorsGs protein-coupled receptorsEffects of drugsDose-response curveHuman beta 2 adrenergic receptorRank order potencyProtein-coupled receptorsReproducible dose-response curvesPharmacological characterizationConcomitant elevationStimulatory guaninePigment dispersionAgonistsIntracellular cAMPImportant receptorReceptorsAntagonistSpecific GsIC50 valuesFunctional expressionCellsXenopus laevis