Featured Publications
Hierarchical deconstruction of mouse olfactory sensory neurons: from whole mucosa to single-cell RNA-seq
Saraiva L, Ibarra-Soria X, Khan M, Omura M, Scialdone A, Mombaerts P, Marioni J, Logan D. Hierarchical deconstruction of mouse olfactory sensory neurons: from whole mucosa to single-cell RNA-seq. Scientific Reports 2015, 5: 18178. PMID: 26670777, PMCID: PMC4680959, DOI: 10.1038/srep18178.Peer-Reviewed Original ResearchConceptsMature olfactory sensory neuronsOlfactory sensory neuronsRNA-seqOR genesGene expressionSingle-cell RNA-seqIntact OR genesOlfactory receptorsCell typesSingle-nucleotide polymorphismsOR-expressing OSNsOR gene expressionIntact olfactory receptorsNeuron-one receptor ruleCrude tissue samplesMonoallelic expressionNeuronal cell typesMouse olfactory mucosaMultiple cell typesChemosensory tissuesGenesCellular heterogeneityOlfactory mucosaSensory neuronsNon-neuronalHigh-throughput spatial mapping of single-cell RNA-seq data to tissue of origin
Achim K, Pettit J, Saraiva L, Gavriouchkina D, Larsson T, Arendt D, Marioni J. High-throughput spatial mapping of single-cell RNA-seq data to tissue of origin. Nature Biotechnology 2015, 33: 503-509. PMID: 25867922, DOI: 10.1038/nbt.3209.Peer-Reviewed Original Research
2023
Genome-Wide RNA Tomography in the Mouse Whole Olfactory Mucosa
Moussa E, Makhlouf M, Mathew L, Saraiva L. Genome-Wide RNA Tomography in the Mouse Whole Olfactory Mucosa. Methods In Molecular Biology 2023, 2710: 19-30. PMID: 37688721, DOI: 10.1007/978-1-0716-3425-7_2.Peer-Reviewed Original Research
2016
Deconstructing Olfaction with Transcriptomics: From Whole Tissue to Single-Cells, and from Zebrafish to Humans
Saraiva L, Ibarra-Soria X, Aguilera F, Marioni J, Logan D. Deconstructing Olfaction with Transcriptomics: From Whole Tissue to Single-Cells, and from Zebrafish to Humans. 2016 DOI: 10.5339/qfarc.2016.hbpp2867.Peer-Reviewed Original ResearchOlfactory sensory neuronsFluorescence assisted cell sortingMature olfactory sensory neuronsMolecular barcodesOlfactory receptorsDegree of molecular conservationOlfactory mucosaCell type-specific genesDynamics of gene expressionInter-specific levelType-specific genesOR gene repertoireSingle-cellGene expression networksOlfactory systemEvolution of olfactionMolecular heterogeneityPopulation of olfactory sensory neuronsCilia of olfactory sensory neuronsReceptive matesSpecies nichesMolecular conservationRNA-seqGene familyGene repertoire