2022
Endothelial Cell TGF-β (Transforming Growth Factor-Beta) Signaling Regulates Venous Adaptive Remodeling to Improve Arteriovenous Fistula Patency
Taniguchi R, Ohashi Y, Lee JS, Hu H, Gonzalez L, Zhang W, Langford J, Matsubara Y, Yatsula B, Tellides G, Fahmy TM, Hoshina K, Dardik A. Endothelial Cell TGF-β (Transforming Growth Factor-Beta) Signaling Regulates Venous Adaptive Remodeling to Improve Arteriovenous Fistula Patency. Arteriosclerosis Thrombosis And Vascular Biology 2022, 42: 868-883. PMID: 35510552, PMCID: PMC9233042, DOI: 10.1161/atvbaha.122.317676.Peer-Reviewed Original ResearchConceptsArteriovenous fistulaSMC proliferationAVF patencyCollagen densityMouse aortocaval fistula modelTGF-β receptor IArteriovenous fistula patencyAortocaval fistula modelInhibition of TGFPredetermined time pointsTGF-β inhibitionTGF-β signalingTGF-β receptorDisruption of TGFFistula patencyAVF failureWall thicknessVascular accessVenous remodelingSuccessful hemodialysisDoppler ultrasoundFistula modelReceptor IPatencyTGF
2021
PD-L1 (Programmed Death Ligand 1) Regulates T-Cell Differentiation to Control Adaptive Venous Remodeling
Matsubara Y, Gonzalez L, Kiwan G, Liu J, Langford J, Gao M, Gao X, Taniguchi R, Yatsula B, Furuyama T, Matsumoto T, Komori K, Mori M, Dardik A. PD-L1 (Programmed Death Ligand 1) Regulates T-Cell Differentiation to Control Adaptive Venous Remodeling. Arteriosclerosis Thrombosis And Vascular Biology 2021, 41: 2909-2922. PMID: 34670406, PMCID: PMC8664128, DOI: 10.1161/atvbaha.121.316380.Peer-Reviewed Original ResearchConceptsPD-L1AVF maturationPD-L1 activityVenous remodelingT cellsL1 antibodyArteriovenous fistulaNude miceMouse aortocaval fistula modelEnd-stage renal diseaseT helper type 2 cellsT helper type 1 (Th1) cellsPrimary success rateSmooth muscle cell proliferationRegulatory T cellsT cell activityT-cell transferAortocaval fistula modelPreferred vascular accessVascular wall thickeningM1-type macrophagesM2-type macrophagesPotential therapeutic targetMuscle cell proliferationType 1 cellsInhibition of T-Cells by Cyclosporine A Reduces Macrophage Accumulation to Regulate Venous Adaptive Remodeling and Increase Arteriovenous Fistula Maturation
Matsubara Y, Kiwan G, Liu J, Gonzalez L, Langford J, Gao M, Gao X, Taniguchi R, Yatsula B, Furuyama T, Matsumoto T, Komori K, Dardik A. Inhibition of T-Cells by Cyclosporine A Reduces Macrophage Accumulation to Regulate Venous Adaptive Remodeling and Increase Arteriovenous Fistula Maturation. Arteriosclerosis Thrombosis And Vascular Biology 2021, 41: e160-e174. PMID: 33472405, PMCID: PMC7904667, DOI: 10.1161/atvbaha.120.315875.Peer-Reviewed Original ResearchConceptsVascular wall thickeningT helper type 1 (Th1) cellsSuccessful AVF maturationAVF maturationRegulatory T cellsMacrophage accumulationT cellsType 1 cellsArteriovenous fistulaVenous remodelingWall thickeningMouse aortocaval fistula modelT helper type 2T helper type 2 cellsAdaptive remodelingM2 macrophage accumulationPrimary success rateAortocaval fistula modelArteriovenous fistula maturationPreferred vascular accessAnti-inflammatory macrophagesT cell functionWild-type miceEffect of CsAType 2 cells
2020
Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae
Wang T, Liu J, Liu H, Lee SR, Gonzalez L, Gorecka J, Shu C, Dardik A. Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae. Journal Of Surgical Research 2020, 262: 224-239. PMID: 33039109, PMCID: PMC8024410, DOI: 10.1016/j.jss.2020.08.071.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthasePhosphorylation of EphrinB2AVF maturationNitric oxide synthaseArteriovenous fistulaVenous remodelingEphB4-FcOxide synthaseEndothelial cell functionEndothelial cell tube formationExtracellular signal-regulated kinases 1/2Murine arteriovenous fistulaSignal-regulated kinases 1/2Cell tube formationAortocaval fistulaHuman endothelial cellsVascular accessExpression of ephrinB2Endothelial cell culture mediumDay 7FistulaEndothelial cellsArterial identityCell functionTube formationNCOA4 is regulated by HIF and mediates mobilization of murine hepatic iron stores after blood loss
Li X, Lozovatsky L, Sukumaran A, Gonzalez L, Jain A, Liu D, Ayala-Lopez N, Finberg KE. NCOA4 is regulated by HIF and mediates mobilization of murine hepatic iron stores after blood loss. Blood 2020, 136: 2691-2702. PMID: 32659785, PMCID: PMC7735158, DOI: 10.1182/blood.2020006321.Peer-Reviewed Original ResearchConceptsHepatic iron storesHypoxia-inducible factorNonheme iron concentrationsIron storesBlood lossNCOA4 expressionSubunit levelsHIF-2α knockdownMurine hepatoma cell lineMessenger RNA inductionDietary ironHepatic responseHepatoma cell lineHIF-1αHepatic originIron deficiencyInducible factorMiceCell linesNCOA4PhlebotomyModest effectRNA inductionIron concentrationProlyl hydroxylases
2019
The potential and limitations of induced pluripotent stem cells to achieve wound healing
Gorecka J, Kostiuk V, Fereydooni A, Gonzalez L, Luo J, Dash B, Isaji T, Ono S, Liu S, Lee SR, Xu J, Liu J, Taniguchi R, Yastula B, Hsia HC, Qyang Y, Dardik A. The potential and limitations of induced pluripotent stem cells to achieve wound healing. Stem Cell Research & Therapy 2019, 10: 87. PMID: 30867069, PMCID: PMC6416973, DOI: 10.1186/s13287-019-1185-1.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsChronic woundsCell therapyStem cellsDiabetic foot ulcersCell typesWound healingInvasive harvesting techniquesAdult-derived stem cellsNormal skin architecturePluripotent stem cellsLimb ischemiaFoot ulcersLimited cell survivalSafety profileOnly cell typeAnimal modelsHuman studiesPotential treatmentPhysiologic responsesHealthy skinImmune rejectionAdvanced therapiesTranslational potentialUse of iPSCsSystemic factors