1977
δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin
Milstone L, Piatigorsky J. δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin. Experimental Cell Research 1977, 105: 9-14. PMID: 837998, DOI: 10.1016/0014-4827(77)90147-1.Peer-Reviewed Original ResearchConceptsEmbryonic lens epitheliumΔ-crystallin synthesisMessenger RNAΔ-crystallinGene expressionLens epitheliumPresence of insulinCultured lens epitheliumFetal calf serumChick lens epitheliumLens epithelial cellsCell elongationTranslational efficiencyCalf serumPolyacrylamide gel electrophoresisTotal protein synthesisProtein synthesisMolecular hybridizationGel electrophoresisSodium dodecyl sulfate-gradient polyacrylamide gel electrophoresisEpithelial cellsSynthesis increasesCellsExpressionHours of culture
1976
δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells
Milstone L, Zelenka P, Piatigorsky J. δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells. Developmental Biology 1976, 48: 197-204. PMID: 1254080, DOI: 10.1016/0012-1606(76)90084-1.Peer-Reviewed Original ResearchConceptsΔ-crystallin synthesisChick lens epithelial cellsChick lens cellsEmbryonic lens epitheliumLens fiber cellsLens epithelial cellsFiber cellsLens cellsDifferential stimulationProtein synthesisCultured cellsMRNALens epitheliumEpithelial cellsTissue cultureCellsDifferentiationOverall stimulationAccumulationStimulationSpecializationCultureSynthesisOvo
1975
Rates of protein synthesis in explanted embryonic chick lens epithelia: Differential stimulation of δ-crystallin synthesis
Milstone L, Piatigorsky J. Rates of protein synthesis in explanted embryonic chick lens epithelia: Differential stimulation of δ-crystallin synthesis. Developmental Biology 1975, 43: 91-100. PMID: 1149925, DOI: 10.1016/0012-1606(75)90133-5.Peer-Reviewed Original ResearchConceptsChick lens epitheliumΔ-crystallin synthesisProtein synthesisΔ-crystallinDifferential protein synthesisLens fiber cellsLens fiber differentiationLens epitheliumProtein degradationFiber cellsFiber differentiationBulk proteinTotal protein synthesisRate of synthesisIntracellular valineLabeling periodNumber of cellsProteinFetal calf serumCellsDifferential stimulationBiochemical changesCalf serumTotal proteinEpithelium