2024
The p. S178L mutation in Tbc1d24 disrupts endosome‐mediated synaptic vesicle trafficking of cochlear hair cells and leads to hearing impairment in mice
Chen P, Hou S, Li G, Lin Y, Lu J, Song L, Li G, Pang X, Wu H, Yang T. The p. S178L mutation in Tbc1d24 disrupts endosome‐mediated synaptic vesicle trafficking of cochlear hair cells and leads to hearing impairment in mice. Clinical Genetics 2024 PMID: 39400345, DOI: 10.1111/cge.14620.Peer-Reviewed Original ResearchNon-syndromic hearing lossSynaptic vesiclesRibbon synapses of cochlear inner hair cellsAutosomal dominant non-syndromic hearing lossDominant non-syndromic hearing lossEndosome-like vacuolesEndosomal membrane traffickingInner hair cellsSynaptic vesicle traffickingHearing lossRibbon synapsesHair cell ribbon synapsesKnock-in mouse modelMutant miceSustained exocytosisVesicle traffickingEndosomal traffickingMembrane traffickingVesicle recyclingIn vitro interactionCochlear inner hair cellsReduced exocytosisWave I amplitudeMild hearing lossAuditory brainstem responseDMXL2 Is Required for Endocytosis and Recycling of Synaptic Vesicles in Auditory Hair Cells
Peng H, Wang L, Gao Y, Liu H, Lin G, Kong Y, Xu P, Liu H, Yuan Q, Liu H, Song L, Yang T, Wu H. DMXL2 Is Required for Endocytosis and Recycling of Synaptic Vesicles in Auditory Hair Cells. Journal Of Neuroscience 2024, 44: e1405232024. PMID: 39147590, PMCID: PMC11411588, DOI: 10.1523/jneurosci.1405-23.2024.Peer-Reviewed Original ResearchEndocytic membrane retrievalSynaptic vesiclesSynaptic exocytosisMembrane retrievalEndocytic compartmentsMolecular machineryRecycling of synaptic vesiclesReserve poolMembrane capacitance measurementsRibbon synapses of inner hair cellsElectron microscopy reconstructionsAuditory synaptopathyMutant miceSynaptic endocytosisCell-specific knockout miceHuman homologMembrane-proximalHair cellsAuditory hair cellsMolecular basisEndocytosisRibbon synapsesInner hair cellsHearing lossDMXL2
2019
Functional alteration of ribbon synapses in inner hair cells by noise exposure causing hidden hearing loss
Liu H, Lu J, Wang Z, Song L, Wang X, Li G, Wu H. Functional alteration of ribbon synapses in inner hair cells by noise exposure causing hidden hearing loss. Neuroscience Letters 2019, 707: 134268. PMID: 31103727, DOI: 10.1016/j.neulet.2019.05.022.Peer-Reviewed Original ResearchConceptsInner hair cellsModerate noise exposureHearing lossRibbon synapsesNoise exposureHair cellsPersistent reductionWhole-cell patch-clamp recordingsNoise-induced hearing lossSynapse functionAuditory afferent fibersHidden hearing lossABR wave ISynaptic vesiclesAuditory brainstem response (ABR) amplitudesPatch-clamp recordingsSevere noise exposureWhole mount immunofluorescence stainingPatch-clamp electrophysiologySensory hair cellsAfferent fibersABR thresholdPersistent alterationsExposure altersFunctional alterationsCellular Differences in the Cochlea of CBA and B6 Mice May Underlie Their Difference in Susceptibility to Hearing Loss
Liu H, Li G, Lu J, Gao Y, Song L, Li G, Wu H. Cellular Differences in the Cochlea of CBA and B6 Mice May Underlie Their Difference in Susceptibility to Hearing Loss. Frontiers In Cellular Neuroscience 2019, 13: 60. PMID: 30873008, PMCID: PMC6400987, DOI: 10.3389/fncel.2019.00060.Peer-Reviewed Original ResearchInner hair cellsEarly-onset hearing lossB6 miceOnset hearing lossHearing lossRibbon synapsesOuter hair cell functionMouse strainsCBA/CaJ miceWave I latencySpiral ganglion neuronsSynaptic vesiclesHair cell functionCellular differencesStable hearingSimilar hearing thresholdsI latencyABR thresholdGanglion neuronsGlutamate toxicityHearing thresholdsWave IMiceHair cellsCell function