2022
Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration
Rizzolo LJ, Nasonkin IO, Adelman RA. Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration. Stem Cells Translational Medicine 2022, 11: 269-281. PMID: 35356975, PMCID: PMC8968686, DOI: 10.1093/stcltm/szac001.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationMacular degenerationPathology of AMDOuter blood-retinal barrierBlood-retinal barrierRetinal pigment epithelium cellsRetinal degenerative conditionsRisky surgical procedureCell replacement mechanismLoss of photoreceptorsRetinal cell transplantationPigment epithelium cellsCell therapy approachesRPE patchesNext-generation therapiesTransplant proceduresCell transplantationRPE transplantsClinical trialsSurgical proceduresApical processesBasal sideDegenerative conditionsRPE apical processesSevere impairment
2021
Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium
Cai H, Gong J, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium. Experimental Eye Research 2021, 207: 108576. PMID: 33895162, DOI: 10.1016/j.exer.2021.108576.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationRetinal pigment epitheliumMacular degenerationPigment epitheliumInduced pluripotent stem cellsEtiology of AMDMitochondrial dysfunctionAge-matched controlsNovel therapeutic targetTranscriptome of fibroblastsAMD patientsNormal donorsFibroblasts of patientsTherapeutic targetPatientsMore studiesAltered transcriptomeDisease phenotypeSignificant differencesCell linesMitochondrial functionDysfunctionOriginal fibroblastsDistinct transcriptomesDegenerationKnockdown of Claudin-19 in the Retinal Pigment Epithelium Is Accompanied by Slowed Phagocytosis and Increased Expression of SQSTM1
Liu F, Peng S, Adelman RA, Rizzolo LJ. Knockdown of Claudin-19 in the Retinal Pigment Epithelium Is Accompanied by Slowed Phagocytosis and Increased Expression of SQSTM1. Investigative Ophthalmology & Visual Science 2021, 62: 14-14. PMID: 33591357, PMCID: PMC7900869, DOI: 10.1167/iovs.62.2.14.Peer-Reviewed Original ResearchConceptsSequestosome 1/p62Photoreceptor outer segmentsInternalized photoreceptor outer segmentsOxidative stress responseApical junctional complexActivation of AMPKTransepithelial electrical resistanceFluorescence-activated cell sortingWestern blottingClaudin-19Expression of proteinsSteady-state levelsProtein kinaseFilamentous actinEffect of AMPStress responseQuantitative RT-PCRKnockdownPOS degradationAMPKJunctional complexesEnzymatic activityCell sortingExpression of metallothioneinProteasome inhibitors
2020
Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium
Singh D, Chen X, Xia T, Ghiassi-Nejad M, Tainsh L, Adelman RA, Rizzolo LJ. Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2020, 61: 9-9. PMID: 33151282, PMCID: PMC7671856, DOI: 10.1167/iovs.61.13.9.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumRetinal progenitor cellsTransepithelial electrical resistanceNeurosensory retinaPigment epitheliumHuman fetal retinal pigment epitheliumRecoverin-positive cellsFetal retinal pigment epitheliumGanglion cell layerAbility of RPERPE signature genesRed/green opsinTranswell filter insertsStem cellsQuantitative RT-PCRGlial markersHuman stem cellsExpression of rhodopsinInterneuron markersBipolar cellsRetinal culturesRetinaRT-PCRProgenitor cellsPresumptive photoreceptorsClaudins regulate gene and protein expression of the retinal pigment epithelium independent of their association with tight junctions
Liu F, Xu T, Peng S, Adelman RA, Rizzolo LJ. Claudins regulate gene and protein expression of the retinal pigment epithelium independent of their association with tight junctions. Experimental Eye Research 2020, 198: 108157. PMID: 32712183, DOI: 10.1016/j.exer.2020.108157.Peer-Reviewed Original ResearchAnalysis of Population Representation Among Willed Whole-Body Donors to Facilitate the Construction of a Body Donation Program in China: From the Perspective of Medical Students and Anatomists
Zhang H, Chen K, Wang N, Zhang D, Zhang Q, Tang K, Wan M, Gong C, Hong X, Qiu W, Rizzolo LJ, Ma C. Analysis of Population Representation Among Willed Whole-Body Donors to Facilitate the Construction of a Body Donation Program in China: From the Perspective of Medical Students and Anatomists. OMEGA - Journal Of Death And Dying 2020, 84: 1146-1159. PMID: 32515268, DOI: 10.1177/0030222820913717.Peer-Reviewed Original ResearchConceptsCause of deathDonation programBody donation programsMedical CollegePeking Union Medical CollegeAge of donorsCerebrovascular diseaseHeart diseaseRespiratory diseaseMedical characteristicsWhole-body donorsNeoplasmsDiseaseDeathCauseBeijing populationAgeDonorsMedical studentsPopulation representationUnstimulated, Serum-free Cultures of Retinal Pigment Epithelium Excrete Large Mounds of Drusen-like Deposits
Chen X, Singh D, Adelman RA, Rizzolo LJ. Unstimulated, Serum-free Cultures of Retinal Pigment Epithelium Excrete Large Mounds of Drusen-like Deposits. Current Eye Research 2020, 45: 1390-1394. PMID: 32202447, DOI: 10.1080/02713683.2020.1740744.Peer-Reviewed Original ResearchMeSH KeywordsActinsApolipoproteins ECalciumCell Culture TechniquesCell LineCell ProliferationCoculture TechniquesCulture Media, Serum-FreeElectric ImpedanceHumansInduced Pluripotent Stem CellsLipid MetabolismRetinal DrusenRetinal Pigment EpitheliumStem CellsTight JunctionsTissue Inhibitor of Metalloproteinase-3VitronectinConceptsRetinal pigment epithelium
2019
Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions
Gong J, Cai H, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions. Stem Cells Translational Medicine 2019, 9: 364-376. PMID: 31840941, PMCID: PMC7031648, DOI: 10.1002/sctm.19-0321.Peer-Reviewed Original ResearchConceptsExtracellular matrixIPSC-RPEMetabolic-related pathwaysComplement immune systemTransepithelial electrical resistanceRod photoreceptor outer segmentsPluripotent stem cellsAged Bruch's membraneCell-specific morphologyObserved phenotypeAltered extracellular matrixControl iPSCsMitochondrial respirationMitochondrial functionMatrix interactionsCell attachmentStem cellsTranscriptomePhotoreceptor outer segmentsDistinct clustersComplement genesRetinal pigment epitheliumGenesIPSCsMembraneInteractions of the choroid, Bruch's membrane, retinal pigment epithelium, and neurosensory retina collaborate to form the outer blood-retinal-barrier
Fields M, Del Priore LV, Adelman RA, Rizzolo LJ. Interactions of the choroid, Bruch's membrane, retinal pigment epithelium, and neurosensory retina collaborate to form the outer blood-retinal-barrier. Progress In Retinal And Eye Research 2019, 76: 100803. PMID: 31704339, DOI: 10.1016/j.preteyeres.2019.100803.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsDisease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function
Wang SB, Xu T, Peng S, Singh D, Ghiassi-Nejad M, Adelman RA, Rizzolo LJ. Disease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function. Communications Biology 2019, 2: 113. PMID: 30937396, PMCID: PMC6433901, DOI: 10.1038/s42003-019-0355-0.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumClaudin-19Retinal neurogenesisP1 waveOuter nuclear layerRPE signature genesARPE19 cell lineOcular involvementKidney diseaseVisual functionFamilial hypomagnesaemiaNuclear layerBipolar cellsNewborn miceOcular diseasesPigment epitheliumRetinal isomeraseDiseaseMiceHuman induced pluripotent cellsRetinal differentiationSignature genesCell linesNeurogenesisInduced pluripotent cells
2017
Neferine, is not inducer but blocker for macroautophagic flux targeting on lysosome malfunction
Xu T, Singh D, Liu J, Li H, Peng S, Rizzolo LJ, Wang SB. Neferine, is not inducer but blocker for macroautophagic flux targeting on lysosome malfunction. Biochemical And Biophysical Research Communications 2017, 495: 1516-1521. PMID: 29197576, DOI: 10.1016/j.bbrc.2017.11.169.Peer-Reviewed Original ResearchConceptsLC3-IIMultiple pharmacological effectsAMPK/mTORNeferine treatmentKnockdown of ATG5Pharmacological effectsCancer cellsNeferineStrong inducerAutophagic fluxCathepsin DPhagocytic cargoLysosome maturationLysosome malfunctionExposureMacroautophagic fluxInducerMaturationAdaptor proteinArrhythmiasBlockersCancerA biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells
Singh D, Wang SB, Xia T, Tainsh L, Ghiassi-Nejad M, Xu T, Peng S, Adelman RA, Rizzolo LJ. A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells. Biomaterials 2017, 154: 158-168. PMID: 29128844, DOI: 10.1016/j.biomaterials.2017.10.052.Peer-Reviewed Original ResearchConceptsRetinal degenerationNeurosensory retinaRetinal cellsHost retinal pigment epitheliumStem cellsOuter nuclear layerRetinal pigment epitheliumRetinal cell typesElaborate arborsQuantitative RT-PCRLeading causeImmune responseNuclear layerMouse modelPigment epitheliumSubretinal spaceForebrain cellsMinimal immune responseTherapeutic agentsRetinal laminationRT-PCRDegenerationRetinaRetinal organoidsConfocal immunocytochemistryEffects of diabetic retinopathy on the barrier functions of the retinal pigment epithelium
Xia T, Rizzolo LJ. Effects of diabetic retinopathy on the barrier functions of the retinal pigment epithelium. Vision Research 2017, 139: 72-81. PMID: 28347688, DOI: 10.1016/j.visres.2017.02.006.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsBlood-retinal barrierRetinal pigment epitheliumOuter blood-retinal barrierRPE barrier functionDiabetic retinopathyBarrier functionPigment epitheliumInner blood-retinal barrierEffect of diabetesRetinal endothelial cellsMicrovascular complicationsDiabetes mellitusNeurosensory retinaRetinal healthParacellular spaceRetinopathyMetabolic processingEndothelial cellsEarly effectsDiabetesActive transportTight junctionsCulture modelEpitheliumTransepithelial movement
2016
Claudin-3 and claudin-19 partially restore native phenotype to ARPE-19 cells via effects on tight junctions and gene expression
Peng S, Wang SB, Singh D, Zhao PY, Davis K, Chen B, Adelman RA, Rizzolo LJ. Claudin-3 and claudin-19 partially restore native phenotype to ARPE-19 cells via effects on tight junctions and gene expression. Experimental Eye Research 2016, 151: 179-189. PMID: 27593915, DOI: 10.1016/j.exer.2016.08.021.Peer-Reviewed Original Research
2015
Human Adult Retinal Pigment Epithelial Stem Cell–Derived RPE Monolayers Exhibit Key Physiological Characteristics of Native TissuePhysiology of Cultured Adult Human RPE
Blenkinsop TA, Saini JS, Maminishkis A, Bharti K, Wan Q, Banzon T, Lotfi M, Davis J, Singh D, Rizzolo LJ, Miller S, Temple S, Stern JH. Human Adult Retinal Pigment Epithelial Stem Cell–Derived RPE Monolayers Exhibit Key Physiological Characteristics of Native TissuePhysiology of Cultured Adult Human RPE. Investigative Ophthalmology & Visual Science 2015, 56: 7085-7099. PMID: 26540654, PMCID: PMC4640474, DOI: 10.1167/iovs.14-16246.Peer-Reviewed Original ResearchTRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment EpitheliumTRP Channels of Human Fetal RPE
Zhao PY, Gan G, Peng S, Wang SB, Chen B, Adelman RA, Rizzolo LJ. TRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment EpitheliumTRP Channels of Human Fetal RPE. Investigative Ophthalmology & Visual Science 2015, 56: 1916-1923. PMID: 25736794, PMCID: PMC4364639, DOI: 10.1167/iovs.14-15738.Peer-Reviewed Original ResearchConceptsHuman fetal RPETRP channelsApical membraneFetal RPEApical plasma membraneCell-cell contactTransepithelial electrical resistanceTransient receptor potential channelsTight junctionsSubcellular localizationInhibitor of calpainPrimary ciliaRT-PCRPlasma membraneBasal channel activityQuantitative RT-PCRApical tight junctionsExpression of TRPC4Ion channelsBasolateral surfaceApical microvilliApical surfaceChannel activityConfocal microscopyTRPM3The student's dilemma, liver edition: Incorporating the sonographer's language into clinical anatomy education
Hall MK, Mirjalili SA, Moore CL, Rizzolo LJ. The student's dilemma, liver edition: Incorporating the sonographer's language into clinical anatomy education. Anatomical Sciences Education 2015, 8: 283-288. PMID: 25573229, DOI: 10.1002/ase.1518.Peer-Reviewed Original ResearchConceptsMain portal fissurePortal fissureCystic pedicleDifficult laparoscopic cholecystectomyMiddle hepatic veinRight hepatic arteryRight upper quadrantImportant clinical roleHepatic arteryLaparoscopic cholecystectomyCystic ductUpper quadrantBiliary systemHepatic veinCadaveric liversExtrahepatic structuresAnatomic variationsClinical roleBlood vesselsLobar fissuresDifferent clinical disciplinesPedicleClinical disciplinesFuture studiesFissures
2014
Barrier properties of cultured retinal pigment epithelium
Rizzolo LJ. Barrier properties of cultured retinal pigment epithelium. Experimental Eye Research 2014, 126: 16-26. PMID: 24731966, DOI: 10.1016/j.exer.2013.12.018.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina
2012
Effects of Proinflammatory Cytokines on the Claudin-19 Rich Tight Junctions of Human Retinal Pigment EpitheliumCytokine Effects on RPE Tight Junctions
Peng S, Gan G, Rao VS, Adelman RA, Rizzolo LJ. Effects of Proinflammatory Cytokines on the Claudin-19 Rich Tight Junctions of Human Retinal Pigment EpitheliumCytokine Effects on RPE Tight Junctions. Investigative Ophthalmology & Visual Science 2012, 53: 5016-5028. PMID: 22761260, PMCID: PMC3410691, DOI: 10.1167/iovs.11-8311.Peer-Reviewed Original ResearchConceptsHuman fetal RPEEffects of TNFαTransepithelial electrical resistanceProinflammatory cytokinesClaudin-19Tight junctionsAge-related macular degenerationZO-1Effects of cytokinesApical sideSubclinical inflammationTumor necrosisMacular degenerationTNFα receptorsOcular diseasesTNFαSerum-free mediumClaudin-3RPE tight junctionsFetal RPECytokinesClaudin-2Confocal immunofluorescence microscopyInhibitor of apoptosis