2020
Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium
Singh D, Chen X, Xia T, Ghiassi-Nejad M, Tainsh L, Adelman RA, Rizzolo LJ. Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2020, 61: 9-9. PMID: 33151282, PMCID: PMC7671856, DOI: 10.1167/iovs.61.13.9.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumRetinal progenitor cellsTransepithelial electrical resistanceNeurosensory retinaPigment epitheliumHuman fetal retinal pigment epitheliumRecoverin-positive cellsFetal retinal pigment epitheliumGanglion cell layerAbility of RPERPE signature genesRed/green opsinTranswell filter insertsStem cellsQuantitative RT-PCRGlial markersHuman stem cellsExpression of rhodopsinInterneuron markersBipolar cellsRetinal culturesRetinaRT-PCRProgenitor cellsPresumptive photoreceptors
2011
Differential Expression of Claudins in Retinas during Normal Development and the Angiogenesis of Oxygen-Induced Retinopathy
Luo Y, Xiao W, Zhu X, Mao Y, Liu X, Chen X, Huang J, Tang S, Rizzolo LJ. Differential Expression of Claudins in Retinas during Normal Development and the Angiogenesis of Oxygen-Induced Retinopathy. Investigative Ophthalmology & Visual Science 2011, 52: 7556-7564. PMID: 21862644, DOI: 10.1167/iovs.11-7185.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlood-Retinal BarrierClaudinsDisease Models, AnimalFluorescent Antibody Technique, IndirectGene Expression Regulation, DevelopmentalHumansInfant, NewbornMembrane ProteinsMiceMice, Inbred C57BLNeovascularization, PhysiologicOccludinOxygenPlant LectinsReal-Time Polymerase Chain ReactionRetinaRetinal NeovascularizationRetinal VesselsRetinopathy of PrematurityRNA, MessengerClaudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium
Peng S, Rao VS, Adelman RA, Rizzolo LJ. Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2011, 52: 1392-1403. PMID: 21071746, PMCID: PMC3101667, DOI: 10.1167/iovs.10-5984.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsCells, CulturedClaudinsElectric ImpedanceFluorescent Antibody Technique, IndirectGene SilencingGestational AgeHumansImmunoblottingMembrane ProteinsOccludinPermeabilityPolyethylene GlycolsRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingTight JunctionsConceptsRetinal pigment epitheliumTransepithelial electrical resistanceHuman retinal pigment epitheliumHuman fetal RPECultured human fetal retinal pigment epitheliumPigment epitheliumClaudin-19Retinal sideHuman fetal retinal pigment epitheliumSpread of edemaFetal retinal pigment epitheliumFetal human retinal pigment epitheliumEffect of serumTight junctionsRPE permeabilityQuantitative RT-PCRChoroidal capillariesSubpopulation of cellsClaudin-1RPE barrierClaudin-3RPE tight junctionsRT-PCREpitheliumSerum
2009
Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions
Peng S, Adelman RA, Rizzolo LJ. Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions. Investigative Ophthalmology & Visual Science 2009, 51: 3216-3225. PMID: 20042644, PMCID: PMC2891474, DOI: 10.1167/iovs.09-4162.Peer-Reviewed Original ResearchAngiogenesis InhibitorsAntibodies, MonoclonalAntibodies, Monoclonal, HumanizedBevacizumabBlood-Retinal BarrierCapillary PermeabilityCells, CulturedClaudinsElectric ImpedanceEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoblottingPolyethylene GlycolsPotassiumRanibizumabRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodiumTight JunctionsVascular Endothelial Growth Factor A
1997
A culture model of development reveals multiple properties of RPE tight junctions.
Ban Y, Rizzolo L. A culture model of development reveals multiple properties of RPE tight junctions. Molecular Vision 1997, 3: 18. PMID: 9479009.Peer-Reviewed Original ResearchAnimalsCell CountCell Membrane PermeabilityChick EmbryoCulture Media, ConditionedCulture TechniquesFluorescent Antibody Technique, IndirectGene Expression Regulation, DevelopmentalHorseradish PeroxidaseInulinMannitolMembrane ProteinsPhalloidinePhosphoproteinsPigment Epithelium of EyeTight JunctionsTime FactorsZonula Occludens-1 ProteinRemodeling of junctional complexes during the development of the outer blood‐retinal barrier
Williams C, Rizzolo L. Remodeling of junctional complexes during the development of the outer blood‐retinal barrier. The Anatomical Record 1997, 249: 380-388. PMID: 9372172, DOI: 10.1002/(sici)1097-0185(199711)249:3<380::aid-ar9>3.0.co;2-y.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumApical junctional complexOuter blood-retinal barrierBlood-retinal barrierNeural retinaZO-1Junctional complexesOccludin expressionRPE developmentMature retinal pigment epitheliumRPE/choroidZO-1 expressionTight junction proteinsEmbryonic day 3Same molecular massEmbryonic day 10Adherens junctionsPigment epitheliumDay 3Day 10Homologous complexesMolecular massRetinaJunction proteinsJunction permeability