2020
Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium
Singh D, Chen X, Xia T, Ghiassi-Nejad M, Tainsh L, Adelman RA, Rizzolo LJ. Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2020, 61: 9-9. PMID: 33151282, PMCID: PMC7671856, DOI: 10.1167/iovs.61.13.9.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumRetinal progenitor cellsTransepithelial electrical resistanceNeurosensory retinaPigment epitheliumHuman fetal retinal pigment epitheliumRecoverin-positive cellsFetal retinal pigment epitheliumGanglion cell layerAbility of RPERPE signature genesRed/green opsinTranswell filter insertsStem cellsQuantitative RT-PCRGlial markersHuman stem cellsExpression of rhodopsinInterneuron markersBipolar cellsRetinal culturesRetinaRT-PCRProgenitor cellsPresumptive photoreceptorsUnstimulated, Serum-free Cultures of Retinal Pigment Epithelium Excrete Large Mounds of Drusen-like Deposits
Chen X, Singh D, Adelman RA, Rizzolo LJ. Unstimulated, Serum-free Cultures of Retinal Pigment Epithelium Excrete Large Mounds of Drusen-like Deposits. Current Eye Research 2020, 45: 1390-1394. PMID: 32202447, DOI: 10.1080/02713683.2020.1740744.Peer-Reviewed Original ResearchMeSH KeywordsActinsApolipoproteins ECalciumCell Culture TechniquesCell LineCell ProliferationCoculture TechniquesCulture Media, Serum-FreeElectric ImpedanceHumansInduced Pluripotent Stem CellsLipid MetabolismRetinal DrusenRetinal Pigment EpitheliumStem CellsTight JunctionsTissue Inhibitor of Metalloproteinase-3VitronectinConceptsRetinal pigment epithelium
2011
Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium
Peng S, Rao VS, Adelman RA, Rizzolo LJ. Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2011, 52: 1392-1403. PMID: 21071746, PMCID: PMC3101667, DOI: 10.1167/iovs.10-5984.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsCells, CulturedClaudinsElectric ImpedanceFluorescent Antibody Technique, IndirectGene SilencingGestational AgeHumansImmunoblottingMembrane ProteinsOccludinPermeabilityPolyethylene GlycolsRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingTight JunctionsConceptsRetinal pigment epitheliumTransepithelial electrical resistanceHuman retinal pigment epitheliumHuman fetal RPECultured human fetal retinal pigment epitheliumPigment epitheliumClaudin-19Retinal sideHuman fetal retinal pigment epitheliumSpread of edemaFetal retinal pigment epitheliumFetal human retinal pigment epitheliumEffect of serumTight junctionsRPE permeabilityQuantitative RT-PCRChoroidal capillariesSubpopulation of cellsClaudin-1RPE barrierClaudin-3RPE tight junctionsRT-PCREpitheliumSerum
2009
Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions
Peng S, Adelman RA, Rizzolo LJ. Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions. Investigative Ophthalmology & Visual Science 2009, 51: 3216-3225. PMID: 20042644, PMCID: PMC2891474, DOI: 10.1167/iovs.09-4162.Peer-Reviewed Original ResearchAngiogenesis InhibitorsAntibodies, MonoclonalAntibodies, Monoclonal, HumanizedBevacizumabBlood-Retinal BarrierCapillary PermeabilityCells, CulturedClaudinsElectric ImpedanceEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoblottingPolyethylene GlycolsPotassiumRanibizumabRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodiumTight JunctionsVascular Endothelial Growth Factor A
2008
Diffusible retinal secretions regulate the expression of tight junctions and other diverse functions of the retinal pigment epithelium.
Sun R, Peng S, Chen X, Zhang H, Rizzolo LJ. Diffusible retinal secretions regulate the expression of tight junctions and other diverse functions of the retinal pigment epithelium. Molecular Vision 2008, 14: 2237-62. PMID: 19057659, PMCID: PMC2593753.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdherens JunctionsAnimalsCell MembraneCells, CulturedChick EmbryoCluster AnalysisCulture Media, ConditionedDiffusionElectric ImpedanceExtracellular MatrixGene Expression ProfilingGene Expression Regulation, DevelopmentalGenomeIon ChannelsKineticsMelaninsMembrane ProteinsMicrotubulesOligonucleotide Array Sequence AnalysisPhagocytosisRetinal Pigment EpitheliumRNA, MessengerSubcellular FractionsTight JunctionsVisual PathwaysConceptsGene expression
2006
Effects of Culture Conditions on Heterogeneity and the Apical Junctional Complex of the ARPE-19 Cell Line
Luo Y, Zhuo Y, Fukuhara M, Rizzolo LJ. Effects of Culture Conditions on Heterogeneity and the Apical Junctional Complex of the ARPE-19 Cell Line. Investigative Ophthalmology & Visual Science 2006, 47: 3644-3655. PMID: 16877439, DOI: 10.1167/iovs.06-0166.Peer-Reviewed Original ResearchCell Culture TechniquesCell Membrane PermeabilityCytoskeletal ProteinsElectric ConductivityElectric ImpedanceElectrophysiologyHorseradish PeroxidaseHumansImmunoblottingImmunohistochemistryMembrane ProteinsMicroscopy, FluorescenceOccludinPigment Epithelium of EyeReverse Transcriptase Polymerase Chain ReactionTight Junctions
2004
The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development
Rahner C, Fukuhara M, Peng S, Kojima S, Rizzolo LJ. The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development. Journal Of Cell Science 2004, 117: 3307-3318. PMID: 15226402, DOI: 10.1242/jcs.01181.Peer-Reviewed Original ResearchAnimalsCell SeparationCells, CulturedChick EmbryoCulture Media, ConditionedDNA PrimersElectric ImpedanceElectrophysiologyFibroblastsFreeze FracturingGene Expression Regulation, DevelopmentalMicroscopy, FluorescenceNerve Tissue ProteinsPigment Epithelium of EyePolymerase Chain ReactionRetinaReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight JunctionsTime Factors
2000
Two secreted retinal factors regulate different stages of development of the outer blood–retinal barrier
Ban Y, Wilt S, Rizzolo L. Two secreted retinal factors regulate different stages of development of the outer blood–retinal barrier. Brain Research 2000, 119: 259-267. PMID: 10675776, DOI: 10.1016/s0165-3806(99)00183-2.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic AgonistsAnimalsBiological TransportBlood-Brain BarrierCatecholaminesCell FractionationCells, CulturedChick EmbryoChromatography, GelCulture Media, ConditionedDopamine AgonistsElectric ImpedanceEndopeptidasesEye ProteinsIndolesPigment Epithelium of EyeSerotonin Receptor AgonistsTight JunctionsConceptsRetinal pigment epitheliumBlood-retinal barrierTransepithelial electrical resistanceOuter blood-retinal barrierBlood-brain barrierEffects of astrocytesCultured retinal pigment epitheliumChick retinal pigment epitheliumPigment epitheliumNeural retinaRetinal factorsChick retinaRPE barrierRPE cellsRetinaEndothelial regionAstrocytesTight junctionsCulture modelDiffusible factorsDifferent stagesActive factorsJunction developmentMultistep processFactors