2022
Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration
Rizzolo LJ, Nasonkin IO, Adelman RA. Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration. Stem Cells Translational Medicine 2022, 11: 269-281. PMID: 35356975, PMCID: PMC8968686, DOI: 10.1093/stcltm/szac001.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationMacular degenerationPathology of AMDOuter blood-retinal barrierBlood-retinal barrierRetinal pigment epithelium cellsRetinal degenerative conditionsRisky surgical procedureCell replacement mechanismLoss of photoreceptorsRetinal cell transplantationPigment epithelium cellsCell therapy approachesRPE patchesNext-generation therapiesTransplant proceduresCell transplantationRPE transplantsClinical trialsSurgical proceduresApical processesBasal sideDegenerative conditionsRPE apical processesSevere impairment
2020
Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium
Singh D, Chen X, Xia T, Ghiassi-Nejad M, Tainsh L, Adelman RA, Rizzolo LJ. Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2020, 61: 9-9. PMID: 33151282, PMCID: PMC7671856, DOI: 10.1167/iovs.61.13.9.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumRetinal progenitor cellsTransepithelial electrical resistanceNeurosensory retinaPigment epitheliumHuman fetal retinal pigment epitheliumRecoverin-positive cellsFetal retinal pigment epitheliumGanglion cell layerAbility of RPERPE signature genesRed/green opsinTranswell filter insertsStem cellsQuantitative RT-PCRGlial markersHuman stem cellsExpression of rhodopsinInterneuron markersBipolar cellsRetinal culturesRetinaRT-PCRProgenitor cellsPresumptive photoreceptors
2019
Disease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function
Wang SB, Xu T, Peng S, Singh D, Ghiassi-Nejad M, Adelman RA, Rizzolo LJ. Disease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function. Communications Biology 2019, 2: 113. PMID: 30937396, PMCID: PMC6433901, DOI: 10.1038/s42003-019-0355-0.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumClaudin-19Retinal neurogenesisP1 waveOuter nuclear layerRPE signature genesARPE19 cell lineOcular involvementKidney diseaseVisual functionFamilial hypomagnesaemiaNuclear layerBipolar cellsNewborn miceOcular diseasesPigment epitheliumRetinal isomeraseDiseaseMiceHuman induced pluripotent cellsRetinal differentiationSignature genesCell linesNeurogenesisInduced pluripotent cells
2017
A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells
Singh D, Wang SB, Xia T, Tainsh L, Ghiassi-Nejad M, Xu T, Peng S, Adelman RA, Rizzolo LJ. A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells. Biomaterials 2017, 154: 158-168. PMID: 29128844, DOI: 10.1016/j.biomaterials.2017.10.052.Peer-Reviewed Original ResearchConceptsRetinal degenerationNeurosensory retinaRetinal cellsHost retinal pigment epitheliumStem cellsOuter nuclear layerRetinal pigment epitheliumRetinal cell typesElaborate arborsQuantitative RT-PCRLeading causeImmune responseNuclear layerMouse modelPigment epitheliumSubretinal spaceForebrain cellsMinimal immune responseTherapeutic agentsRetinal laminationRT-PCRDegenerationRetinaRetinal organoidsConfocal immunocytochemistry
2011
Differential Expression of Claudins in Retinas during Normal Development and the Angiogenesis of Oxygen-Induced Retinopathy
Luo Y, Xiao W, Zhu X, Mao Y, Liu X, Chen X, Huang J, Tang S, Rizzolo LJ. Differential Expression of Claudins in Retinas during Normal Development and the Angiogenesis of Oxygen-Induced Retinopathy. Investigative Ophthalmology & Visual Science 2011, 52: 7556-7564. PMID: 21862644, DOI: 10.1167/iovs.11-7185.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlood-Retinal BarrierClaudinsDisease Models, AnimalFluorescent Antibody Technique, IndirectGene Expression Regulation, DevelopmentalHumansInfant, NewbornMembrane ProteinsMiceMice, Inbred C57BLNeovascularization, PhysiologicOccludinOxygenPlant LectinsReal-Time Polymerase Chain ReactionRetinaRetinal NeovascularizationRetinal VesselsRetinopathy of PrematurityRNA, MessengerIntegration of tight junctions and claudins with the barrier functions of the retinal pigment epithelium
Rizzolo LJ, Peng S, Luo Y, Xiao W. Integration of tight junctions and claudins with the barrier functions of the retinal pigment epithelium. Progress In Retinal And Eye Research 2011, 30: 296-323. PMID: 21704180, DOI: 10.1016/j.preteyeres.2011.06.002.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumOuter retinaPigment epitheliumTight junctionsBarrier functionOuter blood-retinal barrierBlood-retinal barrierHuman retinal pigment epitheliumBlood-tissue barriersTight junctional proteinsPhotoreceptor layerNeural retinaMetabolic alterationsRetinaEpitheliumClaudin compositionTransepithelial diffusionStem cellsJunctional proteinsClaudins
2006
Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions
Luo Y, Fukuhara M, Weitzman M, Rizzolo LJ. Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions. Brain Research 2006, 1110: 55-63. PMID: 16859655, DOI: 10.1016/j.brainres.2006.06.059.Peer-Reviewed Original Research
2004
The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development
Rahner C, Fukuhara M, Peng S, Kojima S, Rizzolo LJ. The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development. Journal Of Cell Science 2004, 117: 3307-3318. PMID: 15226402, DOI: 10.1242/jcs.01181.Peer-Reviewed Original ResearchAnimalsCell SeparationCells, CulturedChick EmbryoCulture Media, ConditionedDNA PrimersElectric ImpedanceElectrophysiologyFibroblastsFreeze FracturingGene Expression Regulation, DevelopmentalMicroscopy, FluorescenceNerve Tissue ProteinsPigment Epithelium of EyePolymerase Chain ReactionRetinaReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight JunctionsTime Factors
1994
The neural retina maintains integrins in the apical membrane of the RPE early in development.
Rizzolo L, Zhou S, Li Z. The neural retina maintains integrins in the apical membrane of the RPE early in development. Investigative Ophthalmology & Visual Science 1994, 35: 2567-76. PMID: 8163344.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumNeural retinaAlpha 6Alpha 3Photoreceptor cell layerSubunit alpha 3Apical membraneBasolateral membraneBeta 1 subunitPigment epitheliumDistribution of integrinsBeta 1 familyRetinaBeta 1Interphotoreceptor matrixExplant culturesCell layerBasal membraneRetinal interfaceEpitheliumIndividual integrinsIntegrin distributionIntegrinsIntercellular interactionsChicken development
1993
Diffusible, retinal factors stimulate the barrier properties of junctional complexes in the retinal pigment epithelium
Rizzolo L, Li Z. Diffusible, retinal factors stimulate the barrier properties of junctional complexes in the retinal pigment epithelium. Journal Of Cell Science 1993, 106: 859-867. PMID: 8308068, DOI: 10.1242/jcs.106.3.859.Peer-Reviewed Original ResearchApical Orientation of the Microtubule Organizing Center and Associated γ-Tubulin during the Polarization of the Retinal Pigment Epithelium in Vivo
Rizzolo L, Joshi H. Apical Orientation of the Microtubule Organizing Center and Associated γ-Tubulin during the Polarization of the Retinal Pigment Epithelium in Vivo. Developmental Biology 1993, 157: 147-156. PMID: 8482407, DOI: 10.1006/dbio.1993.1119.Peer-Reviewed Original ResearchConceptsMicrotubule arraysCell polarityEmbryonic day 3Apical membraneGamma-tubulin fociJunctional complexesSimple epithelial cellsMicrotubule organizing centerDevelopment of epitheliaCentrosomal proteinsJuxtanuclear regionΓ-tubulinUnique reorganizationBasal axisGamma-tubulinCell-basement membrane interactionsFunctional polarityMicrotubule growthMembrane interactionsOrganizing centerPrimary cell culturesCultured cellsEpithelial cellsRetinal pigment epitheliumCell cultures
1991
Basement membrane stimulates the polarized distribution of integrins but not the Na,K-ATPase in the retinal pigment epithelium.
Rizzolo L. Basement membrane stimulates the polarized distribution of integrins but not the Na,K-ATPase in the retinal pigment epithelium. Molecular Biology Of The Cell 1991, 2: 939-949. PMID: 1667092, PMCID: PMC361892, DOI: 10.1091/mbc.2.11.939.Peer-Reviewed Original Research