2024
Retinal Pigmented Epithelium and the Outer Blood-Retinal Barrier
Rizzolo L. Retinal Pigmented Epithelium and the Outer Blood-Retinal Barrier. 2024 DOI: 10.1016/b978-0-443-13820-1.00020-7.Peer-Reviewed Original ResearchRetinal pigment epitheliumOuter blood-retinal barrierBlood-retinal barrierNeurosensory retinaClaudin-19Pigment epitheliumTight junctionsRetinal pigment epithelium functionHuman retinal pigment epitheliumBarrier functionRetinal barrierCo-culture systemClaudin familyParacellular spaceFamily of transmembrane proteinsEpitheliumChoriocapillarisCo-cultureCapillary bedClaudinRetinaBidirectional interactionsGene expressionPhysiological functionsTransmembrane proteins
2020
Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium
Singh D, Chen X, Xia T, Ghiassi-Nejad M, Tainsh L, Adelman RA, Rizzolo LJ. Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2020, 61: 9-9. PMID: 33151282, PMCID: PMC7671856, DOI: 10.1167/iovs.61.13.9.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumRetinal progenitor cellsTransepithelial electrical resistanceNeurosensory retinaPigment epitheliumHuman fetal retinal pigment epitheliumRecoverin-positive cellsFetal retinal pigment epitheliumGanglion cell layerAbility of RPERPE signature genesRed/green opsinTranswell filter insertsStem cellsQuantitative RT-PCRGlial markersHuman stem cellsExpression of rhodopsinInterneuron markersBipolar cellsRetinal culturesRetinaRT-PCRProgenitor cellsPresumptive photoreceptors
2017
A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells
Singh D, Wang SB, Xia T, Tainsh L, Ghiassi-Nejad M, Xu T, Peng S, Adelman RA, Rizzolo LJ. A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells. Biomaterials 2017, 154: 158-168. PMID: 29128844, DOI: 10.1016/j.biomaterials.2017.10.052.Peer-Reviewed Original ResearchConceptsRetinal degenerationNeurosensory retinaRetinal cellsHost retinal pigment epitheliumStem cellsOuter nuclear layerRetinal pigment epitheliumRetinal cell typesElaborate arborsQuantitative RT-PCRLeading causeImmune responseNuclear layerMouse modelPigment epitheliumSubretinal spaceForebrain cellsMinimal immune responseTherapeutic agentsRetinal laminationRT-PCRDegenerationRetinaRetinal organoidsConfocal immunocytochemistry
2011
Integration of tight junctions and claudins with the barrier functions of the retinal pigment epithelium
Rizzolo LJ, Peng S, Luo Y, Xiao W. Integration of tight junctions and claudins with the barrier functions of the retinal pigment epithelium. Progress In Retinal And Eye Research 2011, 30: 296-323. PMID: 21704180, DOI: 10.1016/j.preteyeres.2011.06.002.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumOuter retinaPigment epitheliumTight junctionsBarrier functionOuter blood-retinal barrierBlood-retinal barrierHuman retinal pigment epitheliumBlood-tissue barriersTight junctional proteinsPhotoreceptor layerNeural retinaMetabolic alterationsRetinaEpitheliumClaudin compositionTransepithelial diffusionStem cellsJunctional proteinsClaudins
2008
Glucose Transporters in Retinal Pigment Epithelium Development
Rizzolo L. Glucose Transporters in Retinal Pigment Epithelium Development. Ophthalmology Research 2008, 185-199. DOI: 10.1007/978-1-59745-375-2_10.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsRetinal pigment epitheliumNeural retinaOuter blood-retinal barrierRetinal pigment epithelium developmentBlood-retinal barrierBlood-brain barrierFormation of fenestraeGlucose transporterTight junctionsExpression of GLUT1Systemic endotheliumChoroidal capillariesPigment epitheliumGLUT family membersBasal levelsRetinaEpithelium developmentTranscellular transportTransepithelial transportChoriocapillarisEndothelium
2007
Development and Role of Tight Junctions in the Retinal Pigment Epithelium
Rizzolo LJ. Development and Role of Tight Junctions in the Retinal Pigment Epithelium. International Review Of Cytology 2007, 258: 195-234. PMID: 17338922, DOI: 10.1016/s0074-7696(07)58004-6.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumPigment epitheliumNeural retinaTight junctionsOuter blood-retinal barrierBlood-retinal barrierSlower time courseEpitheliumCell proliferationTissue-specific propertiesTransepithelial diffusionTime courseRetinaParacellular diffusionPhysiological roleEpithelial monolayersSolid tissuesParacellular spaceApical surfaceCulture system
2000
Two secreted retinal factors regulate different stages of development of the outer blood–retinal barrier
Ban Y, Wilt S, Rizzolo L. Two secreted retinal factors regulate different stages of development of the outer blood–retinal barrier. Brain Research 2000, 119: 259-267. PMID: 10675776, DOI: 10.1016/s0165-3806(99)00183-2.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic AgonistsAnimalsBiological TransportBlood-Brain BarrierCatecholaminesCell FractionationCells, CulturedChick EmbryoChromatography, GelCulture Media, ConditionedDopamine AgonistsElectric ImpedanceEndopeptidasesEye ProteinsIndolesPigment Epithelium of EyeSerotonin Receptor AgonistsTight JunctionsConceptsRetinal pigment epitheliumBlood-retinal barrierTransepithelial electrical resistanceOuter blood-retinal barrierBlood-brain barrierEffects of astrocytesCultured retinal pigment epitheliumChick retinal pigment epitheliumPigment epitheliumNeural retinaRetinal factorsChick retinaRPE barrierRPE cellsRetinaEndothelial regionAstrocytesTight junctionsCulture modelDiffusible factorsDifferent stagesActive factorsJunction developmentMultistep processFactors
1997
Remodeling of junctional complexes during the development of the outer blood‐retinal barrier
Williams C, Rizzolo L. Remodeling of junctional complexes during the development of the outer blood‐retinal barrier. The Anatomical Record 1997, 249: 380-388. PMID: 9372172, DOI: 10.1002/(sici)1097-0185(199711)249:3<380::aid-ar9>3.0.co;2-y.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumApical junctional complexOuter blood-retinal barrierBlood-retinal barrierNeural retinaZO-1Junctional complexesOccludin expressionRPE developmentMature retinal pigment epitheliumRPE/choroidZO-1 expressionTight junction proteinsEmbryonic day 3Same molecular massEmbryonic day 10Adherens junctionsPigment epitheliumDay 3Day 10Homologous complexesMolecular massRetinaJunction proteinsJunction permeability
1994
The neural retina maintains integrins in the apical membrane of the RPE early in development.
Rizzolo L, Zhou S, Li Z. The neural retina maintains integrins in the apical membrane of the RPE early in development. Investigative Ophthalmology & Visual Science 1994, 35: 2567-76. PMID: 8163344.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumNeural retinaAlpha 6Alpha 3Photoreceptor cell layerSubunit alpha 3Apical membraneBasolateral membraneBeta 1 subunitPigment epitheliumDistribution of integrinsBeta 1 familyRetinaBeta 1Interphotoreceptor matrixExplant culturesCell layerBasal membraneRetinal interfaceEpitheliumIndividual integrinsIntegrin distributionIntegrinsIntercellular interactionsChicken development