2024
Retinal Pigmented Epithelium and the Outer Blood-Retinal Barrier
Rizzolo L. Retinal Pigmented Epithelium and the Outer Blood-Retinal Barrier. 2024 DOI: 10.1016/b978-0-443-13820-1.00020-7.Peer-Reviewed Original ResearchRetinal pigment epitheliumOuter blood-retinal barrierBlood-retinal barrierNeurosensory retinaClaudin-19Pigment epitheliumTight junctionsRetinal pigment epithelium functionHuman retinal pigment epitheliumBarrier functionRetinal barrierCo-culture systemClaudin familyParacellular spaceFamily of transmembrane proteinsEpitheliumChoriocapillarisCo-cultureCapillary bedClaudinRetinaBidirectional interactionsGene expressionPhysiological functionsTransmembrane proteins
2022
Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration
Rizzolo LJ, Nasonkin IO, Adelman RA. Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration. Stem Cells Translational Medicine 2022, 11: 269-281. PMID: 35356975, PMCID: PMC8968686, DOI: 10.1093/stcltm/szac001.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationMacular degenerationPathology of AMDOuter blood-retinal barrierBlood-retinal barrierRetinal pigment epithelium cellsRetinal degenerative conditionsRisky surgical procedureCell replacement mechanismLoss of photoreceptorsRetinal cell transplantationPigment epithelium cellsCell therapy approachesRPE patchesNext-generation therapiesTransplant proceduresCell transplantationRPE transplantsClinical trialsSurgical proceduresApical processesBasal sideDegenerative conditionsRPE apical processesSevere impairment
2020
RPE Polarity and Barrier Function
Rizzolo L. RPE Polarity and Barrier Function. 2020, 19-45. DOI: 10.1007/978-3-030-28384-1_2.ChaptersCell polarityEpithelial cell polarityPlasma membrane domainsTight junctionsBasolateral plasma membraneRPE polarityCellular processesMembrane domainsPlasma membraneMembrane transportersMembrane transportNeighboring cellsRPE phenotypeSemi-selective barrierProteinBarrier functionJunction proteinsRPE functionParacellular spaceOuter blood-retinal barrierVisual cycleTight junction proteinsTransepithelial electrical resistanceAsymmetric distributionDynamic interplay
2017
Effects of diabetic retinopathy on the barrier functions of the retinal pigment epithelium
Xia T, Rizzolo LJ. Effects of diabetic retinopathy on the barrier functions of the retinal pigment epithelium. Vision Research 2017, 139: 72-81. PMID: 28347688, DOI: 10.1016/j.visres.2017.02.006.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsBlood-retinal barrierRetinal pigment epitheliumOuter blood-retinal barrierRPE barrier functionDiabetic retinopathyBarrier functionPigment epitheliumInner blood-retinal barrierEffect of diabetesRetinal endothelial cellsMicrovascular complicationsDiabetes mellitusNeurosensory retinaRetinal healthParacellular spaceRetinopathyMetabolic processingEndothelial cellsEarly effectsDiabetesActive transportTight junctionsCulture modelEpitheliumTransepithelial movementVascular Biology: RPE Barrier☆
Rizzolo L, Ghiassi-Nejad M, Tainsh L. Vascular Biology: RPE Barrier☆. 2017 DOI: 10.1016/b978-0-12-809324-5.01510-8.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina
2011
Integration of tight junctions and claudins with the barrier functions of the retinal pigment epithelium
Rizzolo LJ, Peng S, Luo Y, Xiao W. Integration of tight junctions and claudins with the barrier functions of the retinal pigment epithelium. Progress In Retinal And Eye Research 2011, 30: 296-323. PMID: 21704180, DOI: 10.1016/j.preteyeres.2011.06.002.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumOuter retinaPigment epitheliumTight junctionsBarrier functionOuter blood-retinal barrierBlood-retinal barrierHuman retinal pigment epitheliumBlood-tissue barriersTight junctional proteinsPhotoreceptor layerNeural retinaMetabolic alterationsRetinaEpitheliumClaudin compositionTransepithelial diffusionStem cellsJunctional proteinsClaudins
2010
Retinal Pigmented Epithelium Barrier
Rizzolo L. Retinal Pigmented Epithelium Barrier. 2010, 101-108. DOI: 10.1016/b978-0-12-374203-2.00132-9.Chapters
2008
Glucose Transporters in Retinal Pigment Epithelium Development
Rizzolo L. Glucose Transporters in Retinal Pigment Epithelium Development. Ophthalmology Research 2008, 185-199. DOI: 10.1007/978-1-59745-375-2_10.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsRetinal pigment epitheliumNeural retinaOuter blood-retinal barrierRetinal pigment epithelium developmentBlood-retinal barrierBlood-brain barrierFormation of fenestraeGlucose transporterTight junctionsExpression of GLUT1Systemic endotheliumChoroidal capillariesPigment epitheliumGLUT family membersBasal levelsRetinaEpithelium developmentTranscellular transportTransepithelial transportChoriocapillarisEndothelium
2007
Development and Role of Tight Junctions in the Retinal Pigment Epithelium
Rizzolo LJ. Development and Role of Tight Junctions in the Retinal Pigment Epithelium. International Review Of Cytology 2007, 258: 195-234. PMID: 17338922, DOI: 10.1016/s0074-7696(07)58004-6.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumPigment epitheliumNeural retinaTight junctionsOuter blood-retinal barrierBlood-retinal barrierSlower time courseEpitheliumCell proliferationTissue-specific propertiesTransepithelial diffusionTime courseRetinaParacellular diffusionPhysiological roleEpithelial monolayersSolid tissuesParacellular spaceApical surfaceCulture system
2000
Two secreted retinal factors regulate different stages of development of the outer blood–retinal barrier
Ban Y, Wilt S, Rizzolo L. Two secreted retinal factors regulate different stages of development of the outer blood–retinal barrier. Brain Research 2000, 119: 259-267. PMID: 10675776, DOI: 10.1016/s0165-3806(99)00183-2.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic AgonistsAnimalsBiological TransportBlood-Brain BarrierCatecholaminesCell FractionationCells, CulturedChick EmbryoChromatography, GelCulture Media, ConditionedDopamine AgonistsElectric ImpedanceEndopeptidasesEye ProteinsIndolesPigment Epithelium of EyeSerotonin Receptor AgonistsTight JunctionsConceptsRetinal pigment epitheliumBlood-retinal barrierTransepithelial electrical resistanceOuter blood-retinal barrierBlood-brain barrierEffects of astrocytesCultured retinal pigment epitheliumChick retinal pigment epitheliumPigment epitheliumNeural retinaRetinal factorsChick retinaRPE barrierRPE cellsRetinaEndothelial regionAstrocytesTight junctionsCulture modelDiffusible factorsDifferent stagesActive factorsJunction developmentMultistep processFactors
1997
Remodeling of junctional complexes during the development of the outer blood‐retinal barrier
Williams C, Rizzolo L. Remodeling of junctional complexes during the development of the outer blood‐retinal barrier. The Anatomical Record 1997, 249: 380-388. PMID: 9372172, DOI: 10.1002/(sici)1097-0185(199711)249:3<380::aid-ar9>3.0.co;2-y.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumApical junctional complexOuter blood-retinal barrierBlood-retinal barrierNeural retinaZO-1Junctional complexesOccludin expressionRPE developmentMature retinal pigment epitheliumRPE/choroidZO-1 expressionTight junction proteinsEmbryonic day 3Same molecular massEmbryonic day 10Adherens junctionsPigment epitheliumDay 3Day 10Homologous complexesMolecular massRetinaJunction proteinsJunction permeability