2020
Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium
Singh D, Chen X, Xia T, Ghiassi-Nejad M, Tainsh L, Adelman RA, Rizzolo LJ. Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2020, 61: 9-9. PMID: 33151282, PMCID: PMC7671856, DOI: 10.1167/iovs.61.13.9.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumRetinal progenitor cellsTransepithelial electrical resistanceNeurosensory retinaPigment epitheliumHuman fetal retinal pigment epitheliumRecoverin-positive cellsFetal retinal pigment epitheliumGanglion cell layerAbility of RPERPE signature genesRed/green opsinTranswell filter insertsStem cellsQuantitative RT-PCRGlial markersHuman stem cellsExpression of rhodopsinInterneuron markersBipolar cellsRetinal culturesRetinaRT-PCRProgenitor cellsPresumptive photoreceptors
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina
2011
Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium
Peng S, Rao VS, Adelman RA, Rizzolo LJ. Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2011, 52: 1392-1403. PMID: 21071746, PMCID: PMC3101667, DOI: 10.1167/iovs.10-5984.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsCells, CulturedClaudinsElectric ImpedanceFluorescent Antibody Technique, IndirectGene SilencingGestational AgeHumansImmunoblottingMembrane ProteinsOccludinPermeabilityPolyethylene GlycolsRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingTight JunctionsConceptsRetinal pigment epitheliumTransepithelial electrical resistanceHuman retinal pigment epitheliumHuman fetal RPECultured human fetal retinal pigment epitheliumPigment epitheliumClaudin-19Retinal sideHuman fetal retinal pigment epitheliumSpread of edemaFetal retinal pigment epitheliumFetal human retinal pigment epitheliumEffect of serumTight junctionsRPE permeabilityQuantitative RT-PCRChoroidal capillariesSubpopulation of cellsClaudin-1RPE barrierClaudin-3RPE tight junctionsRT-PCREpitheliumSerum