2017
Loss of endothelial Fto is protective against metabolic disease
Krüger N, Biwer L, Good M, Rüther U, Isakson B. Loss of endothelial Fto is protective against metabolic disease. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.1015.15.Peer-Reviewed Original ResearchHigh-fat dietObesity-induced metabolic changesHFD-fed miceMyogenic toneBlood pressureNormal chowMesenteric arteryResistance arteriesEndothelial cellsMetabolic changesNC-fed miceObesity-induced hypertensionSerum lipid levelsOrder mesenteric arteriesSerum lipid analysisFto-deficient miceVascular healthVascular levelVascular toneInsulin resistanceInsulin toleranceFat dietPathophysiological changesDeficient miceFat mass
2016
Lipid composition of the myoendothelial junction allows for compartmentalized eNOS function
Biwer L, Taddeo E, Hoehn K, Straub A, Isakson B. Lipid composition of the myoendothelial junction allows for compartmentalized eNOS function. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.727.9.Peer-Reviewed Original ResearchEndothelial nitric oxide synthaseSmooth muscle cellsEndothelial cellsMyoendothelial junctionsResistance arteriesEC endothelial nitric oxide synthaseNeighboring smooth muscle cellsTumor necrosis factor alphaBlood pressure regulationNecrosis factor alphaNitric oxide synthasePKC activityHuman smooth muscle cellsInternal elastic laminaER calcium releaseTHP-1 monocytesCo-cultured endothelial cellsVascular toneENOS functionOxide synthaseFactor alphaGap junction inhibitionLipid compositionDifferential lipid compositionPhenylephrineEndothelial calreticulin and its role in vascular function
Biwer L, Good M, Sonkusare S, Isakson B. Endothelial calreticulin and its role in vascular function. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.955.3.Peer-Reviewed Original ResearchBlood pressureResistance arteriesSmooth muscle cellsER calcium releaseEndothelial cellsMyoendothelial junctionsVascular toneCalcium releaseIntact resistance arteriesPercentage of CD31Microvascular endothelial cellsInternal elastic laminaKnockdown of calreticulinAcute injectionPressure myographyCalcium binding proteinControl miceTamoxifen injectionVascular functionCD31 antibodyKnockout miceCalcium transientsAnatomical hallmarksElastic laminaMuscle cells