Featured Publications
Non–Endoplasmic Reticulum–Based Calr (Calreticulin) Can Coordinate Heterocellular Calcium Signaling and Vascular Function
Biwer L, Good M, Hong K, Patel R, Agrawal N, Looft-Wilson R, Sonkusare S, Isakson B. Non–Endoplasmic Reticulum–Based Calr (Calreticulin) Can Coordinate Heterocellular Calcium Signaling and Vascular Function. Arteriosclerosis Thrombosis And Vascular Biology 2017, 38: 120-130. PMID: 29122814, PMCID: PMC5746467, DOI: 10.1161/atvbaha.117.309886.Peer-Reviewed Original ResearchConceptsThird-order mesenteric arteriesBlood pressureMesenteric arteryVascular functionCalcium signalingIEL holesApplication of CChMyoendothelial junctionsCalcium eventsER calciumSmooth muscle cellsInternal elastic laminaVascular reactivityResistance arteriesSmall arteriesSame arteryKnockout miceArteryΔ miceElastic laminaMuscle cellsEndoplasmic reticulum calciumMiceCALRCalcium signals
2020
Myoendothelial Junctions of Mature Coronary Vessels Express Notch Signaling Proteins
McCallinhart P, Biwer L, Clark O, Isakson B, Lilly B, Trask A. Myoendothelial Junctions of Mature Coronary Vessels Express Notch Signaling Proteins. Frontiers In Physiology 2020, 11: 29. PMID: 32116749, PMCID: PMC7010921, DOI: 10.3389/fphys.2020.00029.Peer-Reviewed Original ResearchInternal elastic laminaMyoendothelial junctionsCoronary circulationCoronary ECsBlood vesselsIntact blood vesselsNotch signalingSmooth muscleMature blood vesselsNotch3 expressionNotch inhibitorsNotch receptorsElastic laminaTranswell insertsCell phenotypeNormal vasculogenesisVSMCsCell contactReceptorsCritical determinantCell-cell contactSignalingVesselsNotch activityCritical sites
2016
Lipid composition of the myoendothelial junction allows for compartmentalized eNOS function
Biwer L, Taddeo E, Hoehn K, Straub A, Isakson B. Lipid composition of the myoendothelial junction allows for compartmentalized eNOS function. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.727.9.Peer-Reviewed Original ResearchEndothelial nitric oxide synthaseSmooth muscle cellsEndothelial cellsMyoendothelial junctionsResistance arteriesEC endothelial nitric oxide synthaseNeighboring smooth muscle cellsTumor necrosis factor alphaBlood pressure regulationNecrosis factor alphaNitric oxide synthasePKC activityHuman smooth muscle cellsInternal elastic laminaER calcium releaseTHP-1 monocytesCo-cultured endothelial cellsVascular toneENOS functionOxide synthaseFactor alphaGap junction inhibitionLipid compositionDifferential lipid compositionPhenylephrineEndothelial calreticulin and its role in vascular function
Biwer L, Good M, Sonkusare S, Isakson B. Endothelial calreticulin and its role in vascular function. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.955.3.Peer-Reviewed Original ResearchBlood pressureResistance arteriesSmooth muscle cellsER calcium releaseEndothelial cellsMyoendothelial junctionsVascular toneCalcium releaseIntact resistance arteriesPercentage of CD31Microvascular endothelial cellsInternal elastic laminaKnockdown of calreticulinAcute injectionPressure myographyCalcium binding proteinControl miceTamoxifen injectionVascular functionCD31 antibodyKnockout miceCalcium transientsAnatomical hallmarksElastic laminaMuscle cells