2017
A Cell Culture Model of Resistance Arteries.
Biwer L, Lechauve C, Vanhoose S, Weiss M, Isakson B. A Cell Culture Model of Resistance Arteries. Journal Of Visualized Experiments 2017 PMID: 28930992, PMCID: PMC5752193, DOI: 10.3791/55992.Peer-Reviewed Original ResearchConceptsCell culture modelCell typesMyoendothelial junctionsEndothelial cell polarizationPlating of cellsIntact cell layerDifferent cell typesSignaling microdomainCulture modelSpecific proteinsCell polarizationPhysiological relevanceActivity assaysProtein isolationSmooth muscle cellsVascular cellsMuscle cellsImmunofluorescent analysisIntact resistance arteriesProteinCellsMuscle fractionsIsolationCell layerVCCC
2015
Endothelial nitric oxide synthase in the microcirculation
Shu X, Keller T, Begandt D, Butcher J, Biwer L, Keller A, Columbus L, Isakson B. Endothelial nitric oxide synthase in the microcirculation. Cellular And Molecular Life Sciences 2015, 72: 4561-4575. PMID: 26390975, PMCID: PMC4628887, DOI: 10.1007/s00018-015-2021-0.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseNitric oxide synthaseOxide synthaseAnti-inflammatory mediatorsRegulation of eNOSRed blood cellsMicrovascular effectsVessel toneImmune responsePharmacological agentsENOS enzymeNitric oxideBlood cellsNO actionKey moleculesENOSVariety of effectsPhysiological roleCellsVasodilatorsSynthaseMicrocirculation