Featured Publications
Endothelial calreticulin deletion impairs endothelial function in aged mice
Biwer L, Askew-Page H, Hong K, Milstein J, Johnstone S, Macal E, Good M, Bagher P, Sonkusare S, Isakson B. Endothelial calreticulin deletion impairs endothelial function in aged mice. AJP Heart And Circulatory Physiology 2020, 318: h1041-h1048. PMID: 32196361, PMCID: PMC7346539, DOI: 10.1152/ajpheart.00586.2019.Peer-Reviewed Original ResearchConceptsVascular functionTauroursodeoxycholic acidMesenteric arteryAged miceCalcium signalsImpaired endothelial-dependent vasodilationThird-order mesenteric arteriesEndothelial cellsEndothelial-dependent vasodilationIncubation of arteriesImpairs endothelial functionSmall resistance arteriesMultifunctional calcium binding proteinER stress inhibitor tauroursodeoxycholic acidWk of ageEndoplasmic reticulum stressPE constrictionEndothelial functionVasodilatory functionResistance arteriesCalcium binding proteinAgonist carbacholClinical trialsMuscarinic stimulationVasodilation
2017
Calreticulin localized to the myoendothelial junction mediates heterocellular communication in resistance arteries
Biwer L, Good M, Hong K, Patel R, Agrawal N, Looft‐Wilson R, Sonkusare S, Isakson B. Calreticulin localized to the myoendothelial junction mediates heterocellular communication in resistance arteries. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.681.12.Peer-Reviewed Original ResearchEndoplasmic reticulumHeterocellular communicationSmooth muscle cellsER retention signal KDELMyoendothelial junctionsExpression of ERCa 2Proteomic screenConditional knockout micePresence of calreticulinSecond messengerCell extensionsER-TrackerBidirectional signalingBinding proteinCalcium binding proteinSignificant enrichmentCalreticulinER calciumAgonist stimulationCytoplasmic contactMuscle cellsProteinKnockout miceSmooth muscle agonists
2016
Endothelial calreticulin and its role in vascular function
Biwer L, Good M, Sonkusare S, Isakson B. Endothelial calreticulin and its role in vascular function. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.955.3.Peer-Reviewed Original ResearchBlood pressureResistance arteriesSmooth muscle cellsER calcium releaseEndothelial cellsMyoendothelial junctionsVascular toneCalcium releaseIntact resistance arteriesPercentage of CD31Microvascular endothelial cellsInternal elastic laminaKnockdown of calreticulinAcute injectionPressure myographyCalcium binding proteinControl miceTamoxifen injectionVascular functionCD31 antibodyKnockout miceCalcium transientsAnatomical hallmarksElastic laminaMuscle cells