2022
A high-throughput assay for directly monitoring nucleolar rRNA biogenesis
Bryant CJ, McCool MA, Abriola L, Surovtseva YV, Baserga SJ. A high-throughput assay for directly monitoring nucleolar rRNA biogenesis. Open Biology 2022, 12: 210305. PMID: 35078352, PMCID: PMC8790372, DOI: 10.1098/rsob.210305.Peer-Reviewed Original ResearchConceptsRibosome biogenesisRRNA biogenesisNucleolar functionSiRNA controlRNA polymerase 1Nucleolar protein fibrillarinPutative small molecule inhibitorsHigh-throughput primary screensHigh-throughput assaysSmall molecule inhibitorsProtein fibrillarinBiogenesisBMH-21Polymerase-1FibrillarinPrimary screenPrimary assayNucleoliAssaysMorphological alterationsRNAP1RibosomopathiesHitsBiological underpinningsDirect quantification
2021
Increased numbers of nucleoli in a genome-wide RNAi screen reveal proteins that link the cell cycle to RNA polymerase I transcription
Ogawa LM, Buhagiar AF, Abriola L, Leland BA, Surovtseva YV, Baserga SJ. Increased numbers of nucleoli in a genome-wide RNAi screen reveal proteins that link the cell cycle to RNA polymerase I transcription. Molecular Biology Of The Cell 2021, 32: 956-973. PMID: 33689394, PMCID: PMC8108525, DOI: 10.1091/mbc.e20-10-0670.Peer-Reviewed Original ResearchConceptsRNA polymerase INumber of nucleoliRibosome biogenesisNucleolar organizer regionsPolymerase ICell cycleRNA polymerase I transcriptionPolymerase I transcriptionCell cycle regulationHigh-throughput quantitative imagingHuman diploid genomeIdentification of proteinsEukaryotic cellsG2/M phaseDiploid genomeNuclear condensatesRibosomal DNACycle regulationHuman breast epithelial cell lineBreast epithelial cell lineI transcriptionNovel regulatorEpithelial cell lineCycle progressionFunctional analysis