Ovarian transplantation with robotic surgery and a neovascularizing human extracellular matrix scaffold: a case series in comparison to meta-analytic data
Oktay K, Marin L, Bedoschi G, Pacheco F, Sugishita Y, Kawahara T, Taylan E, Acosta C, Bang H. Ovarian transplantation with robotic surgery and a neovascularizing human extracellular matrix scaffold: a case series in comparison to meta-analytic data. Fertility And Sterility 2021, 117: 181-192. PMID: 34801235, PMCID: PMC8863399, DOI: 10.1016/j.fertnstert.2021.08.034.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultCohort StudiesCryopreservationExtracellular MatrixFemaleFertility PreservationHumansMeta-Analysis as TopicNeovascularization, PhysiologicOvaryPregnancyPregnancy RateRetrospective StudiesRobotic Surgical ProceduresTissue Culture TechniquesTissue ScaffoldsTransplantation, AutologousYoung AdultConceptsGraft longevityCase seriesOvarian functionLow-dose aspirinOvarian tissue transplantationOvarian cortical piecesLower embryo qualityOocytes/embryosNeonatal outcomesTransdermal estrogenChemotherapy exposureOvarian transplantationCortical piecesMeta-analytic dataLower abdomenHealthy childrenLive birthsMAIN OUTCOMEGraft vascularizationExtracellular matrix scaffoldsOvarian tissueAneuploidy rateFunction returnsEmbryo qualityMeta-analytic updateIndividual-oocyte transcriptomic analysis shows that genotoxic chemotherapy depletes human primordial follicle reserve in vivo by triggering proapoptotic pathways without growth activation
Titus S, Szymanska K, Musul B, Turan V, Taylan E, Garcia- Milian R, Mehta S, Oktay K. Individual-oocyte transcriptomic analysis shows that genotoxic chemotherapy depletes human primordial follicle reserve in vivo by triggering proapoptotic pathways without growth activation. Scientific Reports 2021, 11: 407. PMID: 33431979, PMCID: PMC7801500, DOI: 10.1038/s41598-020-79643-x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntineoplastic Combined Chemotherapy ProtocolsApoptosisCyclophosphamideDNA DamageFemaleGene Expression ProfilingHeterograftsHumansMiceMice, Inbred NODMice, SCIDOocytesOogenesisOvarian FollicleOvarian ReserveOvarySignal TransductionSingle-Cell AnalysisTranscriptomeYoung AdultConceptsPrimordial follicle oocytesFollicle lossGrowth activationHuman ovarian xenograft modelPI3K/PTEN/AktFollicle oocytesDNA damagePI3K/PTEN/Akt pathwayOvarian reserve depletionPrimordial follicle reservePTEN/AKT pathwayIngenuity Pathway AnalysisOvarian xenograft modelPTEN/AKTSevere DNA damageExpression of AktGonadotoxic chemotherapyAnti-apoptotic Bcl2Early menopauseFollicle reserveTranscriptomic analysisCyclophosphamide injectionHuman ovaryPhosphorylation statusRNA sequencing