2015
Factor VIII organisation on nanodiscs with different lipid composition
Grushin K, Miller J, Dalm D, Stoilova-McPhie S. Factor VIII organisation on nanodiscs with different lipid composition. Thrombosis And Haemostasis 2015, 113: 741-749. PMID: 25589466, DOI: 10.1160/th14-09-0725.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsBinding SitesFactor VIIIImage Processing, Computer-AssistedImaging, Three-DimensionalMembrane ProteinsMembranes, ArtificialMicroscopy, Electron, TransmissionNanostructuresPhosphatidylserinesProtein BindingProtein ConformationProtein FoldingProtein MultimerizationRecombinant ProteinsSurface PropertiesSwineConceptsMembrane-bound factor VIIISingle-particle electron microscopyProtein-membrane interfaceElectron microscopyComplex assemblyBinding of FVIIIFVIII formsSingle-particle reconstructionDifferent lipid compositionsFunctional studiesNanodiscsProteinLipid compositionPhysiological conditionsFurther assemblyPhosphatidylserineStructural studiesMembrane patchesMembranePlatelet membranesStructure determinationNormal haemostasisParticle reconstructionComplexesAssemblyDimeric Organization of Blood Coagulation Factor VIII bound to Lipid Nanotubes
Dalm D, Galaz-Montoya J, Miller J, Grushin K, Villalobos A, Koyfman A, Schmid M, Stoilova-McPhie S. Dimeric Organization of Blood Coagulation Factor VIII bound to Lipid Nanotubes. Scientific Reports 2015, 5: 11212. PMID: 26082135, PMCID: PMC4469981, DOI: 10.1038/srep11212.Peer-Reviewed Original ResearchConceptsMembrane-bound factor VIIIMembrane-binding domainCryo-electron microscopyMembrane-bound structuresSerine protease factor IXaDeficiency of FVIIIDimeric organizationSubtomogram averagingComplex assemblyHelical reconstructionLipid nanotubesCritical functionsFactor IXaBlood coagulationBlood coagulation factor VIIIStructural informationCrystal structureCritical stepPlatelet membranesPlatelet surfaceSevere bleeding disorderCoagulation factor VIIIIntermediate resolutionBleeding disorderMembrane
2014
Helical organization of blood coagulation factor VIII on lipid nanotubes.
Miller J, Dalm D, Koyfman A, Grushin K, Stoilova-McPhie S. Helical organization of blood coagulation factor VIII on lipid nanotubes. Journal Of Visualized Experiments 2014 PMID: 24961276, PMCID: PMC4126079, DOI: 10.3791/51254.Peer-Reviewed Original ResearchConceptsCryo-electron microscopyFVIII formsThree-dimensional structure analysisMembrane-bound stateMembrane-bound structuresSerine protease factor IXaBlood coagulation factor VIIIHelical organizationDeficiency of FVIIISequence proteinFunctional complexLipid nanotubesCryo-EMDetailed protocolProteolytic activationLipid nanotechnologyStructure analysisCoagulation factor VIIIFVIII bindsPowerful approachProteinFunctional structureStructural informationPlatelet membranesMembrane
2013
Circadian phase‐dependent effect of nitric oxide on L‐type voltage‐gated calcium channels in avian cone photoreceptors
Ko M, Shi L, Huang C, Grushin K, Park S, Ko G. Circadian phase‐dependent effect of nitric oxide on L‐type voltage‐gated calcium channels in avian cone photoreceptors. Journal Of Neurochemistry 2013, 127: 314-328. PMID: 23895452, PMCID: PMC4112736, DOI: 10.1111/jnc.12384.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCalcium Channels, L-TypeChick EmbryoCircadian RhythmCyclic GMPCyclic GMP-Dependent Protein KinasesImmunoenzyme TechniquesIn Vitro TechniquesMAP Kinase Signaling SystemNitratesNitric OxideNitric Oxide DonorsNitric Oxide SynthaseOncogene Protein v-aktPatch-Clamp TechniquesPhosphatidylinositol 3-KinasesRetinal Cone Photoreceptor CellsRNA, Small InterferingS-Nitroso-N-AcetylpenicillamineSignal TransductionTransfectionConceptsCircadian output pathwayProtein kinase BL-VGCC currentsProtein kinase GL-type voltage-gated calcium channelsRas-mitogen-activated protein kinaseKinase BCircadian controlVoltage-gated calcium channelsPhase-dependent regulationLight-dependent mannerExtracellular signal-regulated kinaseCone photoreceptorsSignal-regulated kinaseProtein expressionCircadian phase-dependent effectEndogenous circadian oscillatorOutput pathwaysPhosphorylation of ERKProtein kinasePhosphorylation stateNeural nitric oxide synthaseSubunit expressionCircadian regulationCircadian oscillator
2010
CIRCADIAN PROFILES IN THE EMBRYONIC CHICK HEART: L-TYPE VOLTAGE-GATED CALCIUM CHANNELS AND SIGNALING PATHWAYS
Ko M, Shi L, Grushin K, Nigussie F, Ko G. CIRCADIAN PROFILES IN THE EMBRYONIC CHICK HEART: L-TYPE VOLTAGE-GATED CALCIUM CHANNELS AND SIGNALING PATHWAYS. Chronobiology International 2010, 27: 1673-1696. PMID: 20969517, PMCID: PMC3155203, DOI: 10.3109/07420528.2010.514631.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiological ClocksCalcium Channels, L-TypeChick EmbryoChickensCircadian RhythmGene Expression RegulationHeartMAP Kinase Kinase KinasesMuscle, Smooth, VascularMyocardiumMyocytes, CardiacP38 Mitogen-Activated Protein KinasesPhosphorylationPolymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSignal TransductionConceptsCircadian oscillatorL-type voltage-gated calcium channelsCircadian regulationCircadian controlStress-activated protein kinaseComplex cellular processesExtracellular signal-regulated kinaseVoltage-gated calcium channelsProtein kinase BSignal-regulated kinaseAdult mammalian cardiomyocytesMetabolic-related genesSynthase kinase-3βMultiple physiological eventsEndogenous circadian oscillatorMammalian embryosCellular processesProtein kinasePhosphorylation stateCircadian clockEmbryonic chick heartKinase BCircadian genesMaternal influenceSignaling pathways
2007
Control of the L-type Ca2+ current by the NO-cGMP cascade in isolated cardiomyocytes of normotensive and spontaneously hypertensive rats
Kokoz Y, Grushin K, Nenov M, Dynnik V, Semushina S, Pakhomova I, Murashev A. Control of the L-type Ca2+ current by the NO-cGMP cascade in isolated cardiomyocytes of normotensive and spontaneously hypertensive rats. Doklady Biochemistry And Biophysics 2007, 415: 170-173. PMID: 17933327, DOI: 10.1134/s1607672907040023.Peer-Reviewed Original Research
2005
Stabilizing Role of Arginine and NO in the Regulation of Voltage-Sensitive L-type Ca2+ Current in Cardiocytes
Dynnik V, Grushin K, Korystova A, Nenov M, Murashov A, Kokoz Y. Stabilizing Role of Arginine and NO in the Regulation of Voltage-Sensitive L-type Ca2+ Current in Cardiocytes. Doklady Biochemistry And Biophysics 2005, 404: 353-356. PMID: 16392755, DOI: 10.1007/s10628-005-0112-9.Peer-Reviewed Original Research