2022
Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release
Grushin K, Kalyana Sundaram RV, Sindelar CV, Rothman JE. Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2121259119. PMID: 35135883, PMCID: PMC8851502, DOI: 10.1073/pnas.2121259119.Peer-Reviewed Original ResearchMeSH KeywordsGene Expression RegulationHEK293 CellsHumansModels, MolecularNerve Tissue ProteinsProtein Conformation
2015
Factor VIII organisation on nanodiscs with different lipid composition
Grushin K, Miller J, Dalm D, Stoilova-McPhie S. Factor VIII organisation on nanodiscs with different lipid composition. Thrombosis And Haemostasis 2015, 113: 741-749. PMID: 25589466, DOI: 10.1160/th14-09-0725.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsBinding SitesFactor VIIIImage Processing, Computer-AssistedImaging, Three-DimensionalMembrane ProteinsMembranes, ArtificialMicroscopy, Electron, TransmissionNanostructuresPhosphatidylserinesProtein BindingProtein ConformationProtein FoldingProtein MultimerizationRecombinant ProteinsSurface PropertiesSwineConceptsMembrane-bound factor VIIISingle-particle electron microscopyProtein-membrane interfaceElectron microscopyComplex assemblyBinding of FVIIIFVIII formsSingle-particle reconstructionDifferent lipid compositionsFunctional studiesNanodiscsProteinLipid compositionPhysiological conditionsFurther assemblyPhosphatidylserineStructural studiesMembrane patchesMembranePlatelet membranesStructure determinationNormal haemostasisParticle reconstructionComplexesAssemblyDimeric Organization of Blood Coagulation Factor VIII bound to Lipid Nanotubes
Dalm D, Galaz-Montoya J, Miller J, Grushin K, Villalobos A, Koyfman A, Schmid M, Stoilova-McPhie S. Dimeric Organization of Blood Coagulation Factor VIII bound to Lipid Nanotubes. Scientific Reports 2015, 5: 11212. PMID: 26082135, PMCID: PMC4469981, DOI: 10.1038/srep11212.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCryoelectron MicroscopyFactor VIIIHumansLipidsModels, MolecularNanotubesProtein BindingProtein ConformationProtein MultimerizationTomographyConceptsMembrane-bound factor VIIIMembrane-binding domainCryo-electron microscopyMembrane-bound structuresSerine protease factor IXaDeficiency of FVIIIDimeric organizationSubtomogram averagingComplex assemblyHelical reconstructionLipid nanotubesCritical functionsFactor IXaBlood coagulationBlood coagulation factor VIIIStructural informationCrystal structureCritical stepPlatelet membranesPlatelet surfaceSevere bleeding disorderCoagulation factor VIIIIntermediate resolutionBleeding disorderMembrane
2014
Lipid nanotechnologies for structural studies of membrane‐associated proteins
Stoilova‐McPhie S, Grushin K, Dalm D, Miller J. Lipid nanotechnologies for structural studies of membrane‐associated proteins. Proteins Structure Function And Bioinformatics 2014, 82: 2902-2909. PMID: 24957666, PMCID: PMC5292012, DOI: 10.1002/prot.24631.Peer-Reviewed Original ResearchMeSH KeywordsCell MembraneCryoelectron MicroscopyFactor VIIIHumansLipid BilayersMembrane ProteinsNanotechnologyNanotubesPhosphatidylserinesProtein ConformationConceptsMembrane-associated proteinsLipid nanotechnologyLipid nanotubesAqueous solutionStructural studiesStructure determinationCryo-EMND technologiesCryo-electron microscopyLipid nanodisksScaffold proteinMembrane curvatureMacromolecular organizationRich membranesLipid bilayersPhysiological environmentProteinNanotechnologyLipid compositionNanotubesPhysiological conditionsAtomic structureFunctional structureProof of principleNanodisks