Helical organization of blood coagulation factor VIII on lipid nanotubes.
Miller J, Dalm D, Koyfman A, Grushin K, Stoilova-McPhie S. Helical organization of blood coagulation factor VIII on lipid nanotubes. Journal Of Visualized Experiments 2014 PMID: 24961276, PMCID: PMC4126079, DOI: 10.3791/51254.Peer-Reviewed Original ResearchConceptsCryo-electron microscopyFVIII formsThree-dimensional structure analysisMembrane-bound stateMembrane-bound structuresSerine protease factor IXaBlood coagulation factor VIIIHelical organizationDeficiency of FVIIISequence proteinFunctional complexLipid nanotubesCryo-EMDetailed protocolProteolytic activationLipid nanotechnologyStructure analysisCoagulation factor VIIIFVIII bindsPowerful approachProteinFunctional structureStructural informationPlatelet membranesMembraneLack of recombinant factor VIII B‐domain induces phospholipid vesicle aggregation: implications for the immunogenicity of factor VIII
Grushin K, Miller J, Dalm D, Parker E, Healey J, Lollar P, Stoilova-McPhie S. Lack of recombinant factor VIII B‐domain induces phospholipid vesicle aggregation: implications for the immunogenicity of factor VIII. Haemophilia 2014, 20: 723-731. PMID: 24750465, PMCID: PMC4149818, DOI: 10.1111/hae.12421.Peer-Reviewed Original ResearchConceptsFVIII formsB domainFVIII-BDDPhospholipid vesicle aggregationFactor VIII B domainCryo-electron microscopyMembrane-bound statePhospholipid vesiclesMembrane-bound formSerine protease factor IXaPhysiological conditionsFVIII B domainSecondary structure distributionCryo-EMVesicle aggregationBiophysical propertiesCircular dichroismProtein therapeuticsVesiclesTenase complexPhospholipid membranesPlatelet surfaceHuman factor VIIIFactor IXaHereditary bleeding disorders