2018
Structural Insight into the Interaction of Synaptotagmin-1 and Snare Complex on Lipid Bilayer by Cryo-Electron Microscopy
Grushin K, Wang J, Coleman J, Rothman J, Sindelar C, Krishnakumar S. Structural Insight into the Interaction of Synaptotagmin-1 and Snare Complex on Lipid Bilayer by Cryo-Electron Microscopy. Biophysical Journal 2018, 114: 282a. DOI: 10.1016/j.bpj.2017.11.1622.Peer-Reviewed Original Research
2016
Conformational Response of Influenza A M2 Transmembrane Domain to Amantadine Drug Binding at Low pH (pH 5.5)
Georgieva E, Borbat P, Grushin K, Stoilova-McPhie S, Kulkarni N, Liang Z, Freed J. Conformational Response of Influenza A M2 Transmembrane Domain to Amantadine Drug Binding at Low pH (pH 5.5). Frontiers In Physiology 2016, 7: 317. PMID: 27524969, PMCID: PMC4965473, DOI: 10.3389/fphys.2016.00317.Peer-Reviewed Original ResearchTransmembrane domainDouble electron-electron resonanceSingle transmembrane helixM2 transmembrane domainCore transmembrane domainNoticeable conformational changesC-terminal sideJuxtamembrane residuesTransmembrane helicesOligomer equilibriumHelix bundleConformational rearrangementsGolgi apparatusConformational responseM2 proteinStable tetramerConformational changesExit poreProton channelChannel closureG34A mutationSpecific mutationsLipid bilayersLow pH environmentViral cycle
2014
Lipid nanotechnologies for structural studies of membrane‐associated proteins
Stoilova‐McPhie S, Grushin K, Dalm D, Miller J. Lipid nanotechnologies for structural studies of membrane‐associated proteins. Proteins Structure Function And Bioinformatics 2014, 82: 2902-2909. PMID: 24957666, PMCID: PMC5292012, DOI: 10.1002/prot.24631.Peer-Reviewed Original ResearchConceptsMembrane-associated proteinsLipid nanotechnologyLipid nanotubesAqueous solutionStructural studiesStructure determinationCryo-EMND technologiesCryo-electron microscopyLipid nanodisksScaffold proteinMembrane curvatureMacromolecular organizationRich membranesLipid bilayersPhysiological environmentProteinNanotechnologyLipid compositionNanotubesPhysiological conditionsAtomic structureFunctional structureProof of principleNanodisks