1995
Regulation of Cytochrome c Oxidase Subunit mRNA and Enzyme Activity in Rat Brain Reward Regions During Withdrawal from Chronic Cocaine
Walker J, Sevarino K. Regulation of Cytochrome c Oxidase Subunit mRNA and Enzyme Activity in Rat Brain Reward Regions During Withdrawal from Chronic Cocaine. Journal Of Neurochemistry 1995, 64: 497-502. PMID: 7830041, DOI: 10.1046/j.1471-4159.1995.64020497.x.Peer-Reviewed Original ResearchConceptsMedial prefrontal cortexNucleus accumbensBrain regionsChronic cocaine treatmentCO activityChronic treatmentCocaine treatmentBrain reward regionsChronic cocaineDrug rewardPrefrontal cortexSubunit mRNAsIndividual ratsReward regionsCocaineNorthern blot analysisBlot analysisTime-dependent patternUnique time-dependent patternEnzyme activityRatsScreening procedureTreatmentMRNAMetabolic activity[8] Cytochrome-c oxidase from Saccharomyces cerevisiae
Poyton R, Goehring B, Droste M, Sevarino K, Allen L, Zhao X. [8] Cytochrome-c oxidase from Saccharomyces cerevisiae. Methods In Enzymology 1995, 260: 97-116. PMID: 8592475, DOI: 10.1016/0076-6879(95)60133-3.Peer-Reviewed Original ResearchMeSH KeywordsCell FractionationChromatography, GelChromatography, Ion ExchangeDetergentsElectron Transport Complex IVElectrophoresis, Polyacrylamide GelGenes, FungalGenome, FungalIndicators and ReagentsMacromolecular SubstancesMitochondriaMolecular WeightSaccharomyces cerevisiaeSolubilitySubmitochondrial ParticlesUltracentrifugationConceptsCatalytic activityHoloenzyme moleculesAmmonium sulfate fractionationTetramethyl-p-phenylenediamine dihydrochlorideDye NSubmitochondrial particlesSulfate fractionationMoleculesCytochrome c oxidasePolarographic assaySpectrophotometric assayDetergent solubilizationType of assayOxidaseChromatography
1984
The nuclear-coded subunits of yeast cytochrome c oxidase. I. Fractionation of the holoenzyme into chemically pure polypeptides and the identification of two new subunits using solvent extraction and reversed phase high performance liquid chromatography.
Power S, Lochrie M, Sevarino K, Patterson T, Poyton R. The nuclear-coded subunits of yeast cytochrome c oxidase. I. Fractionation of the holoenzyme into chemically pure polypeptides and the identification of two new subunits using solvent extraction and reversed phase high performance liquid chromatography. Journal Of Biological Chemistry 1984, 259: 6564-6570. PMID: 6327684, DOI: 10.1016/s0021-9258(20)82178-4.Peer-Reviewed Original Research
1980
PROTEIN PRECURSORS IN THE ASSEMBLY OF YEAST CYTOCHROME c OXIDASE, A TRANSMEMBRANOUS OLIGOMER OF THE INNER MITOCHONDRIAL MEMBRANE*
Poyton R, Sevarino K, George‐Nascimento C, Power S. PROTEIN PRECURSORS IN THE ASSEMBLY OF YEAST CYTOCHROME c OXIDASE, A TRANSMEMBRANOUS OLIGOMER OF THE INNER MITOCHONDRIAL MEMBRANE*. Annals Of The New York Academy Of Sciences 1980, 343: 275-292. PMID: 6249163, DOI: 10.1111/j.1749-6632.1980.tb47258.x.Peer-Reviewed Original ResearchMitochondrial membrane biogenesis: identification of a precursor to yeast cytochrome c oxidase subunit II, an integral polypeptide.
Sevarino K, Poyton R. Mitochondrial membrane biogenesis: identification of a precursor to yeast cytochrome c oxidase subunit II, an integral polypeptide. Proceedings Of The National Academy Of Sciences Of The United States Of America 1980, 77: 142-146. PMID: 6244538, PMCID: PMC348224, DOI: 10.1073/pnas.77.1.142.Peer-Reviewed Original ResearchMeSH KeywordsAurintricarboxylic AcidCell-Free SystemElectron Transport Complex IVIntracellular MembranesMembrane ProteinsMitochondriaPeptide FragmentsProtein PrecursorsSaccharomyces cerevisiaeConceptsMitochondrial translation productsInner mitochondrial membraneTranslation productsMitochondrial membraneIntegral polypeptidesCytochrome c oxidase subunit IIMitochondrial translation systemOligomeric enzyme complexesAurintricarboxylic acidNH2-terminal extensionCytochrome c oxidaseYeast mitochondriaInner membraneLeader sequenceSubunit IIEnzyme complexLarger precursorC oxidaseUnprocessed formPolypeptideTranslation systemMembraneTransient precursorNormal conditionsRibosomes