1997
An anti-χ-1 antibody recognizes a heavily glycosylated protein in rat brain
Ciabarra A, Sevarino K. An anti-χ-1 antibody recognizes a heavily glycosylated protein in rat brain. Brain Research 1997, 46: 85-90. PMID: 9191081, DOI: 10.1016/s0169-328x(96)00275-6.Peer-Reviewed Original Research
1991
Multiple preprosomatostatin sorting signals mediate secretion via discrete cAMP- and tetradecanoylphorbolacetate-responsive pathways.
Sevarino K, Stork P. Multiple preprosomatostatin sorting signals mediate secretion via discrete cAMP- and tetradecanoylphorbolacetate-responsive pathways. Journal Of Biological Chemistry 1991, 266: 18507-18513. PMID: 1680862, DOI: 10.1016/s0021-9258(18)55090-0.Peer-Reviewed Original ResearchConceptsRegulated secretory pathwaySecretory pathwayMutant precursorRat preprosomatostatinVesicular stomatitis virus G proteinMature peptide regionVirus G proteinSorting signalsRegulated secretionAmino terminusProteolytic maturationExpression vectorNeuropeptide precursorsSite recognitionG proteinsRIN-5F cellsPeptide regionPathwaySecreted productsMutationsIntracellular productsCellsEndocrine cellsResidues
1989
Thyrotropin-releasing hormone (TRH) precursor processing Characterization of mature TRH and non-TRH peptides synthesized by transfected mammalian cells*
Sevarino K, Goodman R, Spiess J, Jackson I, Wu P. Thyrotropin-releasing hormone (TRH) precursor processing Characterization of mature TRH and non-TRH peptides synthesized by transfected mammalian cells*. Journal Of Biological Chemistry 1989, 264: 21529-21535. PMID: 2513321, DOI: 10.1016/s0021-9258(20)88217-9.Peer-Reviewed Original ResearchAmino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity
Sevarino K, Stork P, Ventimiglia R, Mandel G, Goodman R. Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity. Cell 1989, 57: 11-19. PMID: 2564811, DOI: 10.1016/0092-8674(89)90167-0.Peer-Reviewed Original ResearchConceptsEndocrine cell linesRat preprosomatostatinCarboxy-terminal thirdDistinct cell typesAmino-terminal sequenceCell linesHybrid proteinLeader sequenceIntracellular targetingRegulated pathwayPreprosomatostatin 1Cellular factorsExpression vectorCell typesPattern of processingProcessing siteBioactive peptidesAnglerfish isletsSequenceDifferential processingPeptidesProteinResiduesPathwayHigh levels
1988
Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line.
Sevarino K, Wu P, Jackson I, Roos B, Mandel G, Goodman R. Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line. Journal Of Biological Chemistry 1988, 263: 620-623. PMID: 3121619, DOI: 10.1016/s0021-9258(19)35397-9.Peer-Reviewed Original ResearchConceptsHypothalamic tissueCA77 cellsThyrotropin-releasing hormoneThyroid carcinoma cell linesRat medullary thyroid carcinoma cell lineMedullary thyroid carcinoma cell lineHormone messenger RNAPost-translational maturationCarcinoma cell linesImmunoreactive TRHRat hypothalamusPromising model systemGene regulationTRH precursorFurther studiesCell linesMessenger RNACell processesModel systemRNATissueRelative levels
1987
Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells.
Sevarino K, Felix R, Banks C, Low M, Montminy M, Mandel G, Goodman R. Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells. Journal Of Biological Chemistry 1987, 262: 4987-4993. PMID: 2881926, DOI: 10.1016/s0021-9258(18)61143-3.Peer-Reviewed Original Research
1986
Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
Montminy M, Sevarino K, Wagner J, Mandel G, Goodman R. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 6682-6686. PMID: 2875459, PMCID: PMC386573, DOI: 10.1073/pnas.83.18.6682.Peer-Reviewed Original ResearchConceptsRat somatostatin geneSomatostatin geneCAMP responsivenessReporter gene encoding chloramphenicolTranscriptional initiation siteProtein kinase 2Promoter deletion mutantsSimian virus 40 promoterSomatostatin gene promoterCAMP-responsive elementPC12 rat pheochromocytoma cellsKinase 2 activityGene encoding chloramphenicolTranscriptional regulationDeletion mutantsChimeric genePromoter elementsGene promoterGene expressionKinase 2Rat pheochromocytoma cellsInitiation siteResponsive elementGenesBase pairs