1996
[4] In Organello mitochondrial protein and RNA synthesis systems from Saccharomyces cereuisiae
Poyton R, Bellus G, McKee E, Sevarino K, Goehring B. [4] In Organello mitochondrial protein and RNA synthesis systems from Saccharomyces cereuisiae. Methods In Enzymology 1996, 264: 36-42. PMID: 8965710, DOI: 10.1016/s0076-6879(96)64006-3.Peer-Reviewed Original ResearchConceptsMitochondrial translation productsTranslation productsMitochondrial gene productsRNase P enzymesMitochondrial gene expressionInner mitochondrial membraneOrganello systemMitochondrial genomeMitochondrial transcriptionMitochondrial translationP enzymesTRNA processingMitochondrial proteinsRNA splicingSmall rRNAProteolytic turnoverRNA synthesis systemRNA componentRespiratory proteinsGene productsMitochondrial membraneTranscription systemGene expressionPolypeptide subunitsRNA synthesis
1995
[8] Cytochrome-c oxidase from Saccharomyces cerevisiae
Poyton R, Goehring B, Droste M, Sevarino K, Allen L, Zhao X. [8] Cytochrome-c oxidase from Saccharomyces cerevisiae. Methods In Enzymology 1995, 260: 97-116. PMID: 8592475, DOI: 10.1016/0076-6879(95)60133-3.Peer-Reviewed Original ResearchMeSH KeywordsCell FractionationChromatography, GelChromatography, Ion ExchangeDetergentsElectron Transport Complex IVElectrophoresis, Polyacrylamide GelGenes, FungalGenome, FungalIndicators and ReagentsMacromolecular SubstancesMitochondriaMolecular WeightSaccharomyces cerevisiaeSolubilitySubmitochondrial ParticlesUltracentrifugationConceptsCatalytic activityHoloenzyme moleculesAmmonium sulfate fractionationTetramethyl-p-phenylenediamine dihydrochlorideDye NSubmitochondrial particlesSulfate fractionationMoleculesCytochrome c oxidasePolarographic assaySpectrophotometric assayDetergent solubilizationType of assayOxidaseChromatography
1984
The nuclear-coded subunits of yeast cytochrome c oxidase. I. Fractionation of the holoenzyme into chemically pure polypeptides and the identification of two new subunits using solvent extraction and reversed phase high performance liquid chromatography.
Power S, Lochrie M, Sevarino K, Patterson T, Poyton R. The nuclear-coded subunits of yeast cytochrome c oxidase. I. Fractionation of the holoenzyme into chemically pure polypeptides and the identification of two new subunits using solvent extraction and reversed phase high performance liquid chromatography. Journal Of Biological Chemistry 1984, 259: 6564-6570. PMID: 6327684, DOI: 10.1016/s0021-9258(20)82178-4.Peer-Reviewed Original Research
1980
PROTEIN PRECURSORS IN THE ASSEMBLY OF YEAST CYTOCHROME c OXIDASE, A TRANSMEMBRANOUS OLIGOMER OF THE INNER MITOCHONDRIAL MEMBRANE*
Poyton R, Sevarino K, George‐Nascimento C, Power S. PROTEIN PRECURSORS IN THE ASSEMBLY OF YEAST CYTOCHROME c OXIDASE, A TRANSMEMBRANOUS OLIGOMER OF THE INNER MITOCHONDRIAL MEMBRANE*. Annals Of The New York Academy Of Sciences 1980, 343: 275-292. PMID: 6249163, DOI: 10.1111/j.1749-6632.1980.tb47258.x.Peer-Reviewed Original ResearchMitochondrial membrane biogenesis: identification of a precursor to yeast cytochrome c oxidase subunit II, an integral polypeptide.
Sevarino K, Poyton R. Mitochondrial membrane biogenesis: identification of a precursor to yeast cytochrome c oxidase subunit II, an integral polypeptide. Proceedings Of The National Academy Of Sciences Of The United States Of America 1980, 77: 142-146. PMID: 6244538, PMCID: PMC348224, DOI: 10.1073/pnas.77.1.142.Peer-Reviewed Original ResearchConceptsMitochondrial translation productsInner mitochondrial membraneTranslation productsMitochondrial membraneIntegral polypeptidesCytochrome c oxidase subunit IIMitochondrial translation systemOligomeric enzyme complexesAurintricarboxylic acidNH2-terminal extensionCytochrome c oxidaseYeast mitochondriaInner membraneLeader sequenceSubunit IIEnzyme complexLarger precursorC oxidaseUnprocessed formPolypeptideTranslation systemMembraneTransient precursorNormal conditionsRibosomes