1991
3 Molecular Assays for Rat Thyrotropin-Releasing Hormone Gene
Lee S, Sevarino K. 3 Molecular Assays for Rat Thyrotropin-Releasing Hormone Gene. International Review Of Neurobiology 1991, 5: 34-52. DOI: 10.1016/b978-0-12-185259-7.50008-9.Peer-Reviewed Original ResearchThyrotropin-releasing hormone geneHormone geneEukaryotic cell linesQuantitation of RNANorthern blot analysisAcetylated speciesTranscriptional regulationGenomic sequencesTransient expressionTranscriptional activityRNA preparationsRNA samplesBlot analysisCell linesGenesMolecular assaysAgarose gelSmall tissue samplesAssaysRNATissue samplesSpeciesThyroid axisLysatesRegulation
1988
Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line.
Sevarino K, Wu P, Jackson I, Roos B, Mandel G, Goodman R. Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line. Journal Of Biological Chemistry 1988, 263: 620-623. PMID: 3121619, DOI: 10.1016/s0021-9258(19)35397-9.Peer-Reviewed Original ResearchConceptsHypothalamic tissueCA77 cellsThyrotropin-releasing hormoneThyroid carcinoma cell linesRat medullary thyroid carcinoma cell lineMedullary thyroid carcinoma cell lineHormone messenger RNAPost-translational maturationCarcinoma cell linesImmunoreactive TRHRat hypothalamusPromising model systemGene regulationTRH precursorFurther studiesCell linesMessenger RNACell processesModel systemRNATissueRelative levels