1997
An anti-χ-1 antibody recognizes a heavily glycosylated protein in rat brain
Ciabarra A, Sevarino K. An anti-χ-1 antibody recognizes a heavily glycosylated protein in rat brain. Brain Research 1997, 46: 85-90. PMID: 9191081, DOI: 10.1016/s0169-328x(96)00275-6.Peer-Reviewed Original Research
1995
Regulation of Cytochrome c Oxidase Subunit mRNA and Enzyme Activity in Rat Brain Reward Regions During Withdrawal from Chronic Cocaine
Walker J, Sevarino K. Regulation of Cytochrome c Oxidase Subunit mRNA and Enzyme Activity in Rat Brain Reward Regions During Withdrawal from Chronic Cocaine. Journal Of Neurochemistry 1995, 64: 497-502. PMID: 7830041, DOI: 10.1046/j.1471-4159.1995.64020497.x.Peer-Reviewed Original ResearchConceptsMedial prefrontal cortexNucleus accumbensBrain regionsChronic cocaine treatmentCO activityChronic treatmentCocaine treatmentBrain reward regionsChronic cocaineDrug rewardPrefrontal cortexSubunit mRNAsIndividual ratsReward regionsCocaineNorthern blot analysisBlot analysisTime-dependent patternUnique time-dependent patternEnzyme activityRatsScreening procedureTreatmentMRNAMetabolic activity
1993
Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line
Nillni E, Sevarino K, Jackson I. Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line. Endocrinology 1993, 132: 1260-1270. DOI: 10.1210/en.132.3.1260.Peer-Reviewed Original ResearchSodium dodecyl sulfate-polyacrylamide gel electrophoresisCryptic peptidesDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisAtT20 cell lineCell linesPulse-chase studiesN-terminal fragmentGel filtration chromatographyCarboxy-terminalCell extractsGel electrophoresisPulse-chaseTRH progenitor sequenceProgenitor sequencePosttranslational processingWestern blot analysisAmino acidsBiosynthetic precursorFiltration chromatographyBlot analysisTumor cell linesGln-His-Pro-GlySequenceProTRH-derived peptides
1991
3 Molecular Assays for Rat Thyrotropin-Releasing Hormone Gene
Lee S, Sevarino K. 3 Molecular Assays for Rat Thyrotropin-Releasing Hormone Gene. International Review Of Neurobiology 1991, 5: 34-52. DOI: 10.1016/b978-0-12-185259-7.50008-9.Peer-Reviewed Original ResearchThyrotropin-releasing hormone geneHormone geneEukaryotic cell linesQuantitation of RNANorthern blot analysisAcetylated speciesTranscriptional regulationGenomic sequencesTransient expressionTranscriptional activityRNA preparationsRNA samplesBlot analysisCell linesGenesMolecular assaysAgarose gelSmall tissue samplesAssaysRNATissue samplesSpeciesThyroid axisLysatesRegulation