2022
Live-Cell Imaging Shows Uneven Segregation of Extrachromosomal DNA Elements and Transcriptionally Active Extrachromosomal DNA Hubs in Cancer
Yi E, Gujar A, Guthrie M, Kim H, Zhao D, Johnson K, Amin S, Costa M, Yu Q, Das S, Jillette N, Clow P, Cheng A, Verhaak R. Live-Cell Imaging Shows Uneven Segregation of Extrachromosomal DNA Elements and Transcriptionally Active Extrachromosomal DNA Hubs in Cancer. Cancer Discovery 2022, 12: 468-483. PMID: 34819316, PMCID: PMC8831456, DOI: 10.1158/2159-8290.cd-21-1376.Peer-Reviewed Original ResearchConceptsExtrachromosomal DNA elementsDNA elementsUneven segregationRNA polymerase IILive-cell imagingPolymerase IIOffspring cellsGene transcriptionCell line modelsEcDNAsRandom segregationGenetic materialLiving cellsCopy numberLive cellsIndividual cellsTumor evolutionMitosisInheritance patternBreakpoint sequencesIssue featureTranscriptionFluorescent markersPatient tissuesCells
2016
5-Hydroxymethylcytosine localizes to enhancer elements and is associated with survival in glioblastoma patients
Johnson K, Houseman E, King J, von Herrmann K, Fadul C, Christensen B. 5-Hydroxymethylcytosine localizes to enhancer elements and is associated with survival in glioblastoma patients. Nature Communications 2016, 7: 13177. PMID: 27886174, PMCID: PMC5133638, DOI: 10.1038/ncomms13177.Peer-Reviewed Original ResearchMeSH Keywords5-MethylcytosineAdultAgedAged, 80 and overBrain NeoplasmsDNA MethylationDNA, NeoplasmEnhancer Elements, GeneticFemaleGene Expression Regulation, NeoplasticGene Regulatory NetworksGlioblastomaHumansIsocitrate DehydrogenaseMaleMiddle AgedPrefrontal CortexSurvival AnalysisTranscription FactorsConceptsRNA regulatory processesTissue-specific transcriptionDisease critical genesMethylation array dataGenomic distributionGenomic localizationCell maintenanceOxidative bisulfite (oxBS) treatmentTranscription factorsEnhancer elementsPrefrontal cortex tissueBisulfite treatmentCellular proliferationRegulatory processesTranscriptionArray dataMolecular alterationsCortex tissueEpigenomeGlioblastomaImmune functionGenesDNARegulationAnnotation