2023
Serum Response Factor Reduces Gene Expression Noise and Confers Cell State Stability
Zhang J, Wu Q, Hu X, Wang Y, Lu J, Chakraborty R, Martin K, Guo S. Serum Response Factor Reduces Gene Expression Noise and Confers Cell State Stability. Stem Cells 2023, 41: 907-915. PMID: 37386941, PMCID: PMC11009695, DOI: 10.1093/stmcls/sxad051.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell DifferentiationGene ExpressionMicePluripotent Stem CellsSerum Response FactorConceptsMouse pluripotent stem cellsSerum response factorPluripotent stem cellsCell fate stabilityRole of SRFGene expression noiseHeterogeneous gene expressionResponse factorStem cellsNaïve pluripotencyCell state heterogeneityLineage primingExpression noiseActin dynamicsCellular statesPluripotent cellsSRF functionCell statesMechanical signalingGene expressionFunctional modulationCentral mediatorSerum-containing culturesState heterogeneityCells
2020
Circular RNA CircMAP3K5 Acts as a MicroRNA-22-3p Sponge to Promote Resolution of Intimal Hyperplasia Via TET2-Mediated Smooth Muscle Cell Differentiation
Zeng Z, Xia L, Fan S, Zheng J, Qin J, Fan X, Liu Y, Tao J, Liu Y, Li K, Ling Z, Bu Y, Martin KA, Hwa J, Liu R, Tang WH. Circular RNA CircMAP3K5 Acts as a MicroRNA-22-3p Sponge to Promote Resolution of Intimal Hyperplasia Via TET2-Mediated Smooth Muscle Cell Differentiation. Circulation 2020, 143: 354-371. PMID: 33207953, DOI: 10.1161/circulationaha.120.049715.Peer-Reviewed Original ResearchConceptsHuman coronary artery smooth muscle cellsTet2 knockout miceCoronary artery smooth muscle cellsArtery smooth muscle cellsCircular RNAsSmooth muscle cellsVascular smooth muscle cellsWire-injured mouse femoral arteriesSmooth muscle cell differentiationCircular RNA profilingMuscle cell differentiationRNA sequencing dataLoss of TET2Coronary heart diseaseVascular SMC differentiationMiR-22-3pPlatelet-derived growth factorKnockout miceSMC differentiationMaster regulatorRNA sequencingRNA profilingPlatelet-derived growth factor-BBGene expressionSequencing dataTCF21
Xie Y, Martin KA. TCF21. Circulation Research 2020, 126: 530-532. PMID: 32078454, PMCID: PMC7041869, DOI: 10.1161/circresaha.120.316533.Peer-Reviewed Original Research
2017
Opposing Actions of AKT (Protein Kinase B) Isoforms in Vascular Smooth Muscle Injury and Therapeutic Response
Jin Y, Xie Y, Ostriker AC, Zhang X, Liu R, Lee MY, Leslie KL, Tang W, Du J, Lee SH, Wang Y, Sessa WC, Hwa J, Yu J, Martin KA. Opposing Actions of AKT (Protein Kinase B) Isoforms in Vascular Smooth Muscle Injury and Therapeutic Response. Arteriosclerosis Thrombosis And Vascular Biology 2017, 37: 2311-2321. PMID: 29025710, PMCID: PMC5699966, DOI: 10.1161/atvbaha.117.310053.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCell Cycle ProteinsCell DifferentiationCell MovementCell ProliferationCells, CulturedDisease Models, AnimalForkhead Transcription FactorsGene Expression RegulationGenetic Predisposition to DiseaseHumansMice, KnockoutMuscle, Smooth, VascularMyocytes, Smooth MuscleNeointimaNuclear ProteinsPhenotypePromoter Regions, GeneticProto-Oncogene Proteins c-aktRNA InterferenceRNA, MessengerSignal TransductionSirolimusTime FactorsTrans-ActivatorsTranscription FactorsTransfectionVascular System InjuriesConceptsIntimal hyperplasiaTherapeutic inhibitionVascular smooth muscle injurySmooth muscle-specific deletionSmooth muscle cell proliferationSystemic vascular diseaseSevere intimal hyperplasiaSmooth muscle injuryNew treatment strategiesWild-type miceAkt isoformsMuscle cell proliferationMuscle-specific deletionMechanism of actionVascular smooth muscle cell differentiationCoronary revascularizationSmooth muscle cell differentiationDiabetes mellitusDiabetic patientsControl miceRapamycin therapyVascular diseaseMuscle injuryTherapeutic responseSevere thrombosis
2015
Phosphorylation of GATA-6 is required for vascular smooth muscle cell differentiation after mTORC1 inhibition
Xie Y, Jin Y, Merenick BL, Ding M, Fetalvero KM, Wagner RJ, Mai A, Gleim S, Tucker DF, Birnbaum MJ, Ballif BA, Luciano AK, Sessa WC, Rzucidlo EM, Powell RJ, Hou L, Zhao H, Hwa J, Yu J, Martin KA. Phosphorylation of GATA-6 is required for vascular smooth muscle cell differentiation after mTORC1 inhibition. Science Signaling 2015, 8: ra44. PMID: 25969542, PMCID: PMC4560350, DOI: 10.1126/scisignal.2005482.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell ProliferationGATA6 Transcription FactorHEK293 CellsHumansMechanistic Target of Rapamycin Complex 1MiceMice, KnockoutMultiprotein ComplexesMuscle ProteinsMuscle, Smooth, VascularMyocytes, Smooth MuscleProto-Oncogene Proteins c-aktTOR Serine-Threonine KinasesConceptsGATA-6Vascular smooth muscle cell differentiationSmooth muscle cell differentiationPhosphorylation-deficient mutantDifferentiation of VSMCsRapamycin complex 1Downstream transcriptional targetsTranscription factor GATA-6Muscle cell differentiationInhibition of mTORC1VSMC hyperplasiaTransactivation of promotersTranscriptional targetsVSMC differentiationNuclear accumulationInduced phosphorylationMechanistic targetReversible differentiationCell differentiationCells undergoDrug targetsInhibition of proliferationPhosphorylationWild-type miceMTORC1
2014
Epigenetic regulation of smooth muscle cell plasticity
Liu R, Leslie KL, Martin KA. Epigenetic regulation of smooth muscle cell plasticity. Biochimica Et Biophysica Acta 2014, 1849: 448-453. PMID: 24937434, PMCID: PMC4552189, DOI: 10.1016/j.bbagrm.2014.06.004.Peer-Reviewed Original ResearchConceptsSMC plasticityEpigenetic regulationSmooth muscle cellsCell plasticitySmooth Muscle Cell PlasticityRegulatory cis elementsCell typesDNA demethylation pathwayLevel of epigeneticsMuscle cell plasticityMature cell typesKey transcription factorMajor cell typesHistone modificationsNovel target moleculesDNA methylationTranscription factorsEnvironmental stressCis elementsPhenotypic statesDemethylation pathwaySMC phenotypeMolecular mechanismsAdult bodyRemarkable plasticity
2013
Ten-Eleven Translocation-2 (TET2) Is a Master Regulator of Smooth Muscle Cell Plasticity
Liu R, Jin Y, Tang WH, Qin L, Zhang X, Tellides G, Hwa J, Yu J, Martin KA. Ten-Eleven Translocation-2 (TET2) Is a Master Regulator of Smooth Muscle Cell Plasticity. Circulation 2013, 128: 2047-2057. PMID: 24077167, PMCID: PMC3899790, DOI: 10.1161/circulationaha.113.002887.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtherosclerosisCell DifferentiationCells, CulturedDioxygenasesDNA-Binding ProteinsEpigenesis, GeneticHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMiceMice, KnockoutMuscle, Smooth, VascularMyocytes, Smooth MuscleNuclear ProteinsPromoter Regions, GeneticProto-Oncogene ProteinsTrans-ActivatorsWound HealingConceptsTen-Eleven Translocation-2SMC differentiationTET2 knockdownSmooth muscle cellsGene expressionTranslocation 2Smooth Muscle Cell PlasticityMaster epigenetic regulatorSMC gene expressionContractile gene expressionMuscle cell plasticityDedifferentiated smooth muscle cellsTET2 overexpressionContractile smooth muscle cellsHuman smooth muscle cellsChromatin accessibilityEpigenetic landscapeSMC plasticityChromatin immunoprecipitationEpigenetic regulatorsEpigenetic mechanismsCell plasticityMaster regulatorSMC phenotypeTranscriptional upregulation
2007
Regulation of vascular smooth muscle cell differentiation
Rzucidlo E, Martin K, Powell R. Regulation of vascular smooth muscle cell differentiation. Journal Of Vascular Surgery 2007, 45: a25-a32. PMID: 17544021, DOI: 10.1016/j.jvs.2007.03.001.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsVascular smooth muscle cell differentiationSmooth muscle cell differentiationMuscle cell differentiationCell differentiationPathogenesis of atherosclerosisMajor human diseasesLocal environmental cuesEnvironmental cuesContractile roleIntimal hyperplasiaDifferentiated stateVascular aneurysmsMolecular mechanismsVascular developmentPhenotypic switchingHuman diseasesVessel wallVSMCCritical roleDifferentiationEssential componentHypertensionAsthmaAtherosclerosisHyperplasia
2005
Endothelial cell activation of the smooth muscle cell phosphoinositide 3-kinase/Akt pathway promotes differentiation
Brown D, Rzucidlo E, Merenick B, Wagner R, Martin K, Powell R. Endothelial cell activation of the smooth muscle cell phosphoinositide 3-kinase/Akt pathway promotes differentiation. Journal Of Vascular Surgery 2005, 41: 509-516. PMID: 15838487, DOI: 10.1016/j.jvs.2004.12.024.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicCattleCell DifferentiationCells, CulturedCoculture TechniquesEndothelial CellsMuscle, Smooth, VascularMyocytes, Smooth MusclePhenotypePhosphatidylinositol 3-KinasesProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktSignal TransductionConceptsEC/SMCDifferentiated SMC phenotypeSMC phenotypeSMC differentiationSmooth muscle cellsAkt pathwayProtein markersProtein kinase AktAdenoviral overexpressionContractile protein markersDominant-negative AktEndothelial cellsOpposite endothelial cellsBlood vessel developmentRapid Akt phosphorylationPI3K/Akt pathwayMuscle cellsWestern blottingKinase AktAbility of ECsActive AktPhosphoinositide 3Kinase activityMolecular signalsSynthetic phenotype
2003
The mTOR/p70 S6K1 pathway regulates vascular smooth muscle cell differentiation
Martin K, Rzucidlo E, Merenick B, Fingar D, Brown D, Wagner R, Powell R. The mTOR/p70 S6K1 pathway regulates vascular smooth muscle cell differentiation. American Journal Of Physiology - Cell Physiology 2003, 286: c507-c517. PMID: 14592809, DOI: 10.1152/ajpcell.00201.2003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicBiomarkersCattleCell Cycle ProteinsCell DifferentiationCells, CulturedCyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent Kinase Inhibitor p27CyclinsEndothelium, VascularExtracellular Matrix ProteinsImmunosuppressive AgentsMuscle ContractionMuscle, Smooth, VascularPhenotypeProtein KinasesRibosomal Protein S6 Kinases, 70-kDaSignal TransductionSirolimusTOR Serine-Threonine KinasesTumor Suppressor ProteinsConceptsVascular smooth muscle cellsVSMC differentiationVascular smooth muscle cell differentiationSmooth muscle cell differentiationVSMC gene expressionRapamycin-sensitive mTORMuscle cell differentiationContractile morphologyCyclin-dependent kinase inhibitorCell cycle withdrawalExtracellular matrix protein synthesisContractile proteinsMTOR pathway inhibitor rapamycinMuscle alpha-actinTranscriptional controlMatrix protein synthesisNovel functionGene expressionMigratory phenotypeRapamycin inductionMultiple speciesCell differentiationInhibitor rapamycinS6K1 pathwayProtein synthesis
1997
Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes
Clifford E, Martin K, Dalal P, Thomas R, Dubyak G. Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes. American Journal Of Physiology 1997, 273: c973-c987. PMID: 9316419, DOI: 10.1152/ajpcell.1997.273.3.c973.Peer-Reviewed Original ResearchMeSH Keywords5'-NucleotidaseAdenosine DiphosphateAdenosine MonophosphateAdenosine TriphosphatasesAdenosine TriphosphateAntigens, CDApyraseBucladesineCell DifferentiationCytosolDNA PrimersGene Expression Regulation, NeoplasticHL-60 CellsHumansLeukemiaLeukocytesModels, BiologicalPolymerase Chain ReactionReceptors, Purinergic P2RNA, MessengerTetradecanoylphorbol AcetateTime FactorsTranscription, GeneticTumor Cells, CulturedUridine TriphosphateConceptsKG-1 cellsMyeloid progenitorsStage-specific expressionHL-60 promyelocytesExtracellular ATPMyeloid cell differentiationHL-60P2-purinergic receptor subtypesSignal transductionMyeloid leukocytesHL-60 cellsTranscription-polymerase chain reaction analysisATP receptor subtypesExogenous ATPaseCell differentiationRecent identificationDevelopmental stagesApyrase activityTHP-1 monocytesChain reaction analysisPotato apyraseP2Y receptor subtypesPurinergic receptor subtypesDifferentiationAutocrine mechanismDown-Regulation of P2U-Purinergic Nucleotide Receptor Messenger RNA Expression During In Vitro Differentiation of Human Myeloid Leukocytes by Phorbol Esters or Inflammatory Activators
Martin K, Kertesy S, Dubyak G. Down-Regulation of P2U-Purinergic Nucleotide Receptor Messenger RNA Expression During In Vitro Differentiation of Human Myeloid Leukocytes by Phorbol Esters or Inflammatory Activators. Molecular Pharmacology 1997, 51: 97-108. PMID: 9016351, DOI: 10.1124/mol.51.1.97.Peer-Reviewed Original ResearchConceptsTHP-1 monocytesHL-60 cellsMRNA levelsMyeloid leukocytesReceptor messenger RNA expressionMonocyte/macrophage phenotypeMessenger RNA expressionInositol phospholipid hydrolysisNeutrophil phenotypeInflammatory activationHL-60 granulocytesUndifferentiated HL-60 cellsInflammatory activatorsInflammatory macrophagesAcute exposureMacrophage phenotypeNucleotide receptorsHuman epithelial cellsTissue-specific mechanismsEpithelial cellsRNA expressionLeukocytesDown regulationDibutyryl cAMPHuman keratinocytes