2005
Endothelial cell activation of the smooth muscle cell phosphoinositide 3-kinase/Akt pathway promotes differentiation
Brown D, Rzucidlo E, Merenick B, Wagner R, Martin K, Powell R. Endothelial cell activation of the smooth muscle cell phosphoinositide 3-kinase/Akt pathway promotes differentiation. Journal Of Vascular Surgery 2005, 41: 509-516. PMID: 15838487, DOI: 10.1016/j.jvs.2004.12.024.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicCattleCell DifferentiationCells, CulturedCoculture TechniquesEndothelial CellsMuscle, Smooth, VascularMyocytes, Smooth MusclePhenotypePhosphatidylinositol 3-KinasesProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktSignal TransductionConceptsEC/SMCDifferentiated SMC phenotypeSMC phenotypeSMC differentiationSmooth muscle cellsAkt pathwayProtein markersProtein kinase AktAdenoviral overexpressionContractile protein markersDominant-negative AktEndothelial cellsOpposite endothelial cellsBlood vessel developmentRapid Akt phosphorylationPI3K/Akt pathwayMuscle cellsWestern blottingKinase AktAbility of ECsActive AktPhosphoinositide 3Kinase activityMolecular signalsSynthetic phenotype
1999
p70 S6 Kinase Is Regulated by Protein Kinase Cζ and Participates in a Phosphoinositide 3-Kinase-Regulated Signalling Complex
Romanelli A, Martin K, Toker A, Blenis J. p70 S6 Kinase Is Regulated by Protein Kinase Cζ and Participates in a Phosphoinositide 3-Kinase-Regulated Signalling Complex. Molecular And Cellular Biology 1999, 19: 2921-2928. PMID: 10082559, PMCID: PMC84086, DOI: 10.1128/mcb.19.4.2921.Peer-Reviewed Original ResearchConceptsP70 S6 kinasePDK-1Protein kinase CS6 kinaseGrowth factor-independent mannerK activationPhosphoinositide-dependent kinase 1Atypical protein kinase CC-terminal truncation mutantsPDK-1 activationProtein kinase CζFactor-independent mannerThreonine 389Pseudosubstrate domainSignaling ComplexGrowth factorSequential phosphorylationTruncation mutantsCoexpression experimentsEpidermal growth factorCatalytic loopUpstream regulatorKinase 1PKC isoformsKinase C