2024
Abstract 1147: Crosstalk Between Alk5 And Mtorc1 Signaling Promotes VSMC Differentiation And The Therapeutic Effect Of Rapamycin
Chakraborty R, Chatterjee P, Dave J, Obrien B, Joshi D, Schulz V, Greif D, Hwa J, Gallagher P, Martin K. Abstract 1147: Crosstalk Between Alk5 And Mtorc1 Signaling Promotes VSMC Differentiation And The Therapeutic Effect Of Rapamycin. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: a1147-a1147. DOI: 10.1161/atvb.44.suppl_1.1147.Peer-Reviewed Original ResearchVascular smooth muscle cellsTherapeutic effect of rapamycinEffects of rapamycinVSMC differentiationContractile genesConsistent with in vitro findingsRapamycin treatmentCarotid artery injuryHuman coronary artery SMCsVascular smooth muscle cell differentiationIntimal hyperplasiaSmooth muscle cellsCoronary artery SMCsMTORC1 inhibitor rapamycinPhosphorylation of Smad2/3Inhibition of ALK5Smad-binding elementSmad transcription factorsALK5 activityArterial injuryArtery SMCsKnockout miceInhibition of mTORC1Vascular smooth muscle cell plasticityMuscle cells
2022
A guide to molecular and functional investigations of platelets to bridge basic and clinical sciences
Tyagi T, Jain K, Gu S, Qiu M, Gu V, Melchinger H, Rinder H, Martin K, Gardiner E, Lee A, Tang W, Hwa J. A guide to molecular and functional investigations of platelets to bridge basic and clinical sciences. Nature Cardiovascular Research 2022, 1: 223-237. PMID: 37502132, PMCID: PMC10373053, DOI: 10.1038/s44161-022-00021-z.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsVascular smooth muscle cellsPlatelet functional assaysCoronavirus disease 2019Smooth muscle cellsImmune cellsImmune regulationVascular remodelingDisease 2019Pathophysiological processesTranslational relevancePatient diagnosisFlow cytometryMuscle cellsPlatelet biologyFunctional assaysPlatelet investigationsHomeostatic processesPlateletsPhenotypic heterogeneityFunctional stateClinical scienceCellsAdditional roleThrombosisSuch diverse functions
2020
Circular RNA CircMAP3K5 Acts as a MicroRNA-22-3p Sponge to Promote Resolution of Intimal Hyperplasia Via TET2-Mediated Smooth Muscle Cell Differentiation
Zeng Z, Xia L, Fan S, Zheng J, Qin J, Fan X, Liu Y, Tao J, Liu Y, Li K, Ling Z, Bu Y, Martin KA, Hwa J, Liu R, Tang WH. Circular RNA CircMAP3K5 Acts as a MicroRNA-22-3p Sponge to Promote Resolution of Intimal Hyperplasia Via TET2-Mediated Smooth Muscle Cell Differentiation. Circulation 2020, 143: 354-371. PMID: 33207953, DOI: 10.1161/circulationaha.120.049715.Peer-Reviewed Original ResearchConceptsHuman coronary artery smooth muscle cellsTet2 knockout miceCoronary artery smooth muscle cellsArtery smooth muscle cellsCircular RNAsSmooth muscle cellsVascular smooth muscle cellsWire-injured mouse femoral arteriesSmooth muscle cell differentiationCircular RNA profilingMuscle cell differentiationRNA sequencing dataLoss of TET2Coronary heart diseaseVascular SMC differentiationMiR-22-3pPlatelet-derived growth factorKnockout miceSMC differentiationMaster regulatorRNA sequencingRNA profilingPlatelet-derived growth factor-BBGene expressionSequencing data
2018
TCF7L2 (Transcription Factor 7-Like 2) Regulation of GATA6 (GATA-Binding Protein 6)-Dependent and -Independent Vascular Smooth Muscle Cell Plasticity and Intimal Hyperplasia
Srivastava R, Rolyan H, Xie Y, Li N, Bhat N, Hong L, Esteghamat F, Adeniran A, Geirsson A, Zhang J, Ge G, Nobrega M, Martin KA, Mani A. TCF7L2 (Transcription Factor 7-Like 2) Regulation of GATA6 (GATA-Binding Protein 6)-Dependent and -Independent Vascular Smooth Muscle Cell Plasticity and Intimal Hyperplasia. Arteriosclerosis Thrombosis And Vascular Biology 2018, 39: 250-262. PMID: 30567484, PMCID: PMC6365015, DOI: 10.1161/atvbaha.118.311830.Peer-Reviewed Original ResearchConceptsInjury-induced intimal hyperplasiaIntimal hyperplasiaObstructive coronary artery diseaseVascular smooth muscle cell dedifferentiationSmooth muscle cell dedifferentiationVascular Smooth Muscle Cell PlasticityLRP6 mutant miceOverexpression of TCF7L2Coronary artery diseaseVascular smooth muscle cellsMultiple mouse modelsMuscle cell dedifferentiationWild-type littermatesSmooth muscle cellsRole of TCF7L2Smooth Muscle Cell PlasticityVascular smooth muscle cell differentiationMuscle cell plasticitySmooth muscle cell differentiationArtery diseaseSM-MHCMouse modelCell cycle inhibitorsHaploinsufficient miceHyperplasia
2017
Coordinating Regulation of Gene Expression in Cardiovascular Disease: Interactions between Chromatin Modifiers and Transcription Factors
Bauer AJ, Martin KA. Coordinating Regulation of Gene Expression in Cardiovascular Disease: Interactions between Chromatin Modifiers and Transcription Factors. Frontiers In Cardiovascular Medicine 2017, 4: 19. PMID: 28428957, PMCID: PMC5382160, DOI: 10.3389/fcvm.2017.00019.Peer-Reviewed Original ResearchChromatin modifiersTranscription factorsGene expressionTranscriptional controlChromatin-modifying proteinsTranscription factor recruitmentDysregulated gene expressionNumerous cell typesChromatin structureFactor recruitmentHistone methylationGene accessibilityTranscriptional interactionsDNA methylationEpigenetic modifiersRegulatory regionsHistone acetylationVascular smooth muscle cellsCell typesSmooth muscle cellsMuscle cellsMethylationNovel therapeuticsExpressionDisease pathogenesis
2010
Adiponectin Is Secreted by Vascular Smooth Muscle Cells and Regulates Muscle Contractile Phenotype
Ding M, Wagner R, Martin K. Adiponectin Is Secreted by Vascular Smooth Muscle Cells and Regulates Muscle Contractile Phenotype. The FASEB Journal 2010, 24: 957.5-957.5. DOI: 10.1096/fasebj.24.1_supplement.957.5.Peer-Reviewed Original ResearchAdiponectin overexpressionContractile phenotypeVSMC contractile proteinsVascular smooth muscle cellsSmooth muscle cellsContractile protein expressionVascular smooth muscle cell differentiationVSMC contractile phenotypeCardioprotective adipokineSmooth muscle cell differentiationVisceral fatReceptors AdipoR1Endogenous adiponectinRecombinant adiponectinAdiponectinParacrine actionCardioprotective functionParacrine mannerVSMC proliferationHigher order oligomeric formsSiRNA knock-downMuscle cell differentiationMuscle cellsContractile proteinsVSMC6.05 In Vitro Vascular Cell Culture Systems – Vascular Smooth Muscle
Martin K, Rzucidlo E, Ding M, Merenick B, Kasza Z, Wagner R, Powell R. 6.05 In Vitro Vascular Cell Culture Systems – Vascular Smooth Muscle. 2010, 69-96. DOI: 10.1016/b978-0-08-046884-6.00705-3.ChaptersVascular smooth muscle cellsPhenotypic modulationInsulin-like growth factor IEndothelial cellsVSMC differentiationPathogenesis of atherosclerosisRapamycin (mTOR) inhibitor rapamycinSerum response factorDedifferentiated vascular smooth muscle cellsGrowth factor INormal vascular physiologyActivated T cellsSmooth muscle cellsContractile protein expressionEffects of drugsT cellsSmooth muscleIntimal hyperplasiaFactor family membersTumor angiogenesisVascular physiologyMature arteriesFactor IMuscle cellsVSMC phenotype
2006
The prostacyclin receptor induces human vascular smooth muscle cell differentiation via PKA
Fetalvero K, Shyu M, Nomikos A, Chiu Y, Wagner R, Powell R, Hwa J, Martin K. The prostacyclin receptor induces human vascular smooth muscle cell differentiation via PKA. The FASEB Journal 2006, 20: a665-a666. DOI: 10.1096/fasebj.20.4.a665-d.Peer-Reviewed Original ResearchVascular smooth muscle cellsHuman vascular smooth muscle cellsCardiovascular eventsHuman vascular smooth muscle cell differentiationProstacyclin receptorVSMC phenotypeSelective COX-2 inhibitorsAdverse cardiovascular eventsSuperficial femoral arteryProstacyclin analogue iloprostCOX-2 inhibitorsSmooth muscle cellsCAMP/PKA signalingVascular smooth muscle cell differentiationSmooth muscle differentiation markersSmooth muscle cell differentiationArterial injuryCardioprotective effectsFemoral arteryAnalogue iloprostClinical trialsH-caldesmonOrgan donorsAnimal modelsNM iloprost
2003
The mTOR/p70 S6K1 pathway regulates vascular smooth muscle cell differentiation
Martin K, Rzucidlo E, Merenick B, Fingar D, Brown D, Wagner R, Powell R. The mTOR/p70 S6K1 pathway regulates vascular smooth muscle cell differentiation. American Journal Of Physiology - Cell Physiology 2003, 286: c507-c517. PMID: 14592809, DOI: 10.1152/ajpcell.00201.2003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicBiomarkersCattleCell Cycle ProteinsCell DifferentiationCells, CulturedCyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent Kinase Inhibitor p27CyclinsEndothelium, VascularExtracellular Matrix ProteinsImmunosuppressive AgentsMuscle ContractionMuscle, Smooth, VascularPhenotypeProtein KinasesRibosomal Protein S6 Kinases, 70-kDaSignal TransductionSirolimusTOR Serine-Threonine KinasesTumor Suppressor ProteinsConceptsVascular smooth muscle cellsVSMC differentiationVascular smooth muscle cell differentiationSmooth muscle cell differentiationVSMC gene expressionRapamycin-sensitive mTORMuscle cell differentiationContractile morphologyCyclin-dependent kinase inhibitorCell cycle withdrawalExtracellular matrix protein synthesisContractile proteinsMTOR pathway inhibitor rapamycinMuscle alpha-actinTranscriptional controlMatrix protein synthesisNovel functionGene expressionMigratory phenotypeRapamycin inductionMultiple speciesCell differentiationInhibitor rapamycinS6K1 pathwayProtein synthesis
1997
Angiotensin II activates the beta 1 isoform of phospholipase C in vascular smooth muscle cells
Schelling J, Nkemere N, Konieczkowski M, Martin K, Dubyak G. Angiotensin II activates the beta 1 isoform of phospholipase C in vascular smooth muscle cells. American Journal Of Physiology 1997, 272: c1558-c1566. PMID: 9176147, DOI: 10.1152/ajpcell.1997.272.5.c1558.Peer-Reviewed Original ResearchConceptsAortic vascular smooth muscle cellsVascular smooth muscle cellsHuman aortic vascular smooth muscle cellsPLC-beta 1Ang IISmooth muscle cellsPLC beta isoformsPLC-beta 1 antibodyRat aortic vascular smooth muscle cellsPermeabilized vascular smooth muscle cellsMuscle cellsGrowth factorPathophysiology of hypertensionAngiotensin II receptorsIP formationReverse transcriptase-polymerase chain reactionPLC-gamma 1 antibodyTranscriptase-polymerase chain reactionPlatelet-derived growth factorCytosolic free Ca2Gamma 1 antibodiesAng II signalsAngiotensin IIAlpha antibodyII receptors