2021
An Unanticipated Role for Sphingosine Kinase-2 in Bone and in the Anabolic Effect of Parathyroid Hormone
Walker JM, Yao GQ, Siu E, Zhu M, Sun BH, Simpson C, Insogna KL. An Unanticipated Role for Sphingosine Kinase-2 in Bone and in the Anabolic Effect of Parathyroid Hormone. Endocrinology 2021, 162: bqab042. PMID: 33640975, PMCID: PMC8095390, DOI: 10.1210/endocr/bqab042.Peer-Reviewed Original ResearchConceptsSphk2-/- miceParathyroid hormoneAnabolic responseFemoral trabecular BV/TVLower spinal bone mineral densityTrabecular BV/TVSpinal bone mineral densityDaily parathyroid hormoneFemoral bone volumeSuppression of sclerostinEnd of treatmentNormal bone massBone mineral densityNormal bone remodelingRole of SphK1BV/TVFemale control animalsSphingosine kinase 2Sphingosine kinaseControl miceLow BMDAnabolic effectsBone massMineral densityControl animalsSelective deletion of the receptor for CSF1, c-fms, in osteoclasts results in a high bone mass phenotype, smaller osteoclasts in vivo and an impaired response to an anabolic PTH regimen
Zhu M, Sun BH, Nevius E, Kaplan J, Pereira J, Insogna K. Selective deletion of the receptor for CSF1, c-fms, in osteoclasts results in a high bone mass phenotype, smaller osteoclasts in vivo and an impaired response to an anabolic PTH regimen. PLOS ONE 2021, 16: e0247199. PMID: 33607650, PMCID: PMC7895546, DOI: 10.1371/journal.pone.0247199.Peer-Reviewed Original ResearchConceptsColony stimulating factor 1Mature osteoclastsBone massHigh bone mass phenotypeAttenuated anabolic responseDaily subcutaneous dosesTrabecular bone massBone mass phenotypeC-fmsNormal tooth eruptionTrabecular bone compartmentsMature osteoclast functionCathepsin K promoterFemurs of maleWild-type animalsOcS/BSFemale knockSubcutaneous dosesNormal weightSmall osteoclastsImpaired responseTrabecular numberAnabolic responseFlox miceMass phenotype
2017
Breast cancer-associated gene 3 interacts with Rac1 and augments NF-κB signaling in vitro, but has no effect on RANKL-induced bone resorption in vivo
Yao C, Yu KP, Philbrick W, Sun BH, Simpson C, Zhang C, Insogna K. Breast cancer-associated gene 3 interacts with Rac1 and augments NF-κB signaling in vitro, but has no effect on RANKL-induced bone resorption in vivo. International Journal Of Molecular Medicine 2017, 40: 1067-1077. PMID: 28791343, PMCID: PMC5593463, DOI: 10.3892/ijmm.2017.3091.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBone ResorptionCathepsin KCell LineFemaleFemurFibroblastsGene Expression RegulationHEK293 CellsHeLa CellsHumansMaleMiceMice, Inbred C57BLMice, TransgenicNeuropeptidesNF-kappa BOrgan SpecificityOsteoclastsPromoter Regions, GeneticRac1 GTP-Binding ProteinRANK LigandSignal TransductionTibiaConceptsNF-κB signalingCell type-dependent roleCritical downstream targetNF-κBCanonical NF-κB signalingNuclear factorReceptor activatorNuclear Rac1Adaptor proteinCancer-associated genesMature osteoclast formationSmall GTPaseDownstream targetsExogenous receptor activatorLow-dose RANKLNF-κB interactionTransgenic animalsImportant regulatorBreast cancer-associated genesWild-type littermatesCell typesRac1SignalingBCA3Dependent role
2015
AgRP Neurons Regulate Bone Mass
Kim JG, Sun BH, Dietrich MO, Koch M, Yao GQ, Diano S, Insogna K, Horvath TL. AgRP Neurons Regulate Bone Mass. Cell Reports 2015, 13: 8-14. PMID: 26411686, PMCID: PMC5868421, DOI: 10.1016/j.celrep.2015.08.070.Peer-Reviewed Original ResearchMeSH KeywordsAgouti-Related ProteinAnimalsArcuate Nucleus of HypothalamusBone DensityBone Diseases, MetabolicFemurGene Expression RegulationHomeostasisHypothalamusIon ChannelsLeptinMaleMiceMice, KnockoutMitochondrial ProteinsNeuronsNorepinephrinePhenotypePropranololReceptors, Adrenergic, betaReceptors, LeptinSignal TransductionSirtuin 1TibiaUncoupling Protein 2ConceptsAgRP neuronsCell-autonomous deletionSignificant regulatory roleAgRP neuronal functionBone massLeptin receptor deletionSkeletal bone metabolismTransgenic animalsRegulatory roleGene deletionBone homeostasisDeletionNeuronal functionPostnatal deletionSympathetic toneReceptor deletionArcuate nucleusLeptin actionBone metabolismSkeletal metabolismMultiple linesNeuronsMiceMetabolismCircuit integrity
2013
An investigation of the association between omega 3 FA and bone mineral density among older adults: results from the National Health and Nutrition Examination Survey years 2005–2008
Mangano KM, Kerstetter JE, Kenny AM, Insogna KL, Walsh SJ. An investigation of the association between omega 3 FA and bone mineral density among older adults: results from the National Health and Nutrition Examination Survey years 2005–2008. Osteoporosis International 2013, 25: 1033-1041. PMID: 24121998, PMCID: PMC3969769, DOI: 10.1007/s00198-013-2501-8.Peer-Reviewed Original ResearchConceptsSpine bone mineral densityLumbar spine bone mineral densityBone mineral densityTotal femur bone mineral densityFemoral neck bone mineral densityFemur bone mineral densityNeck bone mineral densityFA supplement useSupplement useMineral densityFemoral neckNational HealthOlder adultsDietary omega-3 fatty acid intakeGreater femoral neck BMDHigher spine bone mineral densityHigher lumbar spine bone mineral densityOmega-3 fatty acid intakeDietary omega 3 FAHip bone mineral densityOmega-3 intakeFatty acid intakeNutrition Examination SurveyOmega-3 FAMultivariable regression models
2008
Expression and Synthesis of Bone Morphogenetic Proteins by Osteoclasts: A Possible Path to Anabolic Bone Remodeling
Garimella R, Tague SE, Zhang J, Belibi F, Nahar N, Sun BH, Insogna K, Wang J, Anderson HC. Expression and Synthesis of Bone Morphogenetic Proteins by Osteoclasts: A Possible Path to Anabolic Bone Remodeling. Journal Of Histochemistry & Cytochemistry 2008, 56: 569-577. PMID: 18319273, PMCID: PMC2386763, DOI: 10.1369/jhc.2008.950394.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAnimalsBlotting, WesternBone Marrow CellsBone Morphogenetic ProteinsBone RemodelingCoculture TechniquesFemurImmunohistochemistryIn Situ HybridizationIsoenzymesMiceOsteoclastsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSkullTartrate-Resistant Acid PhosphataseTibiaConceptsBone morphogenetic proteinBMP-2Morphogenetic proteinsBMP-6 mRNAExpression of transcriptsMouse calvarial osteoblastsDifferentiation of osteoblastsBMP-4Osteoclast lysatesOrchestrated processIntercellular communicationReal-time PCRCalvarial osteoblastsBone marrow cellsSitu hybridizationDirect rolePossible direct roleProteinWestern blottingBone remodeling siteBone formationOsteoblastsAnabolic boneMarrow cellsExpression
2003
The Cell Surface Form of Colony-Stimulating Factor-1 Is Biologically Active in Bone in Vivo
Yao GQ, Wu JJ, Sun BH, Troiano N, Mitnick MA, Insogna K. The Cell Surface Form of Colony-Stimulating Factor-1 Is Biologically Active in Bone in Vivo. Endocrinology 2003, 144: 3677-3682. PMID: 12865350, DOI: 10.1210/en.2002-221071.Peer-Reviewed Original ResearchConceptsOp/op miceWild-type miceOp miceBone densityTooth eruptionTransgenic micePeripheral quantitative computed tomographyMCSF-1Factor 1Number of osteoclastsQuantitative computed tomographyOp/op animalsMolar tooth eruptionWild-type animalsOP animalsComputed tomographyColony-stimulating factor-1Colony stimulating factor 1Normal incisorsHistomorphometric analysisTorus Palatinus: A New Anatomical Correlation with Bone Density in Postmenopausal Women
Belsky JL, Hamer JS, Hubert JE, Insogna K, Johns W. Torus Palatinus: A New Anatomical Correlation with Bone Density in Postmenopausal Women. The Journal Of Clinical Endocrinology & Metabolism 2003, 88: 2081-2086. PMID: 12727958, DOI: 10.1210/jc.2002-021726.Peer-Reviewed Original ResearchConceptsBone densityTorus palatinusPostmenopausal womenZ-scoreDual energy x-ray absortiometryNormal postmenopausal womenPostmenopausal female subjectsBody mass indexBone mineral densityBone density measurementsBone density assessmentHigher bone densityMass indexCaucasian womenMineral densityCommunity physiciansUnselected populationAnatomical correlationLumbar vertebraeFemale subjectsDensity assessmentPalatinusOral exostosesWomenPossible correlation
2002
Circulating Levels of Interleukin-6 Soluble Receptor Predict Rates of Bone Loss in Patients with Primary Hyperparathyroidism
Nakchbandi IA, Mitnick MA, Lang R, Gundberg C, Kinder B, Insogna K. Circulating Levels of Interleukin-6 Soluble Receptor Predict Rates of Bone Loss in Patients with Primary Hyperparathyroidism. The Journal Of Clinical Endocrinology & Metabolism 2002, 87: 4946-4951. PMID: 12414855, DOI: 10.1210/jc.2001-011814.Peer-Reviewed Original ResearchConceptsBone lossIL-6sRTotal femurIL-6IL-6 soluble receptorPrimary hyperparathyroidism resultsYearly bone lossSubset of patientsUntreated hyperparathyroidismDisease activityPrimary hyperparathyroidismUpper tertileCytokine axisHyperparathyroidismSoluble receptorPatientsBiochemical markersClassical biochemical markersWhole groupFemurPredict rateGreater rateRecent studiesTertileGroup
2000
Low Dietary Protein and Low Bone Density
Kerstetter JE, Looker AC, Insogna KL. Low Dietary Protein and Low Bone Density. Calcified Tissue International 2000, 66: 313-313. PMID: 10742451, DOI: 10.1007/s002230010062.Peer-Reviewed Original Research
1996
Effects of continuous glucocorticoid infusion on bone metabolism in the rat
King CS, Weir EC, Gundberg CW, Fox J, Insogna KL. Effects of continuous glucocorticoid infusion on bone metabolism in the rat. Calcified Tissue International 1996, 59: 184-191. PMID: 8694896, DOI: 10.1007/s002239900107.Peer-Reviewed Original ResearchConceptsDEX treatmentBone metabolismUrinary calcium/creatinine ratioCalcium/creatinine ratioIntact parathyroid functionLevels of PTHSprague-Dawley ratsTartrate-resistant acid phosphataseParathyroid functionTime-dependent mannerCreatinine ratioSerum calciumBone turnoverContinuous administrationParathyroidectomized animalsSerum concentrationsSupraphysiologic dosesGlucocorticoid infusionDawley ratsFemoral weightHistomorphometric findingsBone massPTX animalsBone volumeDexamethasone