2013
Increasing dietary protein acutely augments intestinal iron transporter expression and significantly increases iron absorption in rats
Thomas CE, Gaffney‐Stomberg E, Sun B, O'Brien KO, Kerstetter JE, Insogna KL. Increasing dietary protein acutely augments intestinal iron transporter expression and significantly increases iron absorption in rats. The FASEB Journal 2013, 27: 2476-2483. PMID: 23447582, DOI: 10.1096/fj.12-221945.Peer-Reviewed Original ResearchConceptsIntestinal Fe absorptionDietary proteinFe absorptionIron transporter expressionTranscript expressionDuodenal mucosaAcute effectsSprague-DawleyStable iron isotopesIron absorptionShort-term effectsIsocaloric dietsIron deficiencyTransporter expressionLittle dataTransferrin receptorExpression of genesSeparate studiesGroupCurrent studyBalance studiesExpressionWhole genome profilingDawleyMucosa
2011
Dietary protein upregulates fractional intestinal iron absorption and directly increases DMT1 transcript and protein expression
Cucchi C, Gaffney‐Stomberg E, Sun B, O'Brien K, Kerstetter J, Insogna K. Dietary protein upregulates fractional intestinal iron absorption and directly increases DMT1 transcript and protein expression. The FASEB Journal 2011, 25: lb193-lb193. DOI: 10.1096/fasebj.25.1_supplement.lb193.Peer-Reviewed Original ResearchProtein dietDietary proteinProtein expressionCaco-2 BBe cellsIntestinal Fe absorptionFe absorption studiesIntestinal iron absorptionTranscript expressionAmino acid-free mediumDMT1 protein levelsAmino acid treatmentDMT1 protein expressionProtein doseIron absorptionBBE cellsIron deficiencyAcid-free mediumRatsProtein levelsDietDMT1 transcriptsAmino acidsFe absorptionWestern analysisKey regulator
1996
Tumor necrosis factor‐α induces transcription of the colony‐stimulating factor‐1 gene in murine osteoblasts
Kaplan D, Eielson C, Horowitz M, Insogna K, Weir E. Tumor necrosis factor‐α induces transcription of the colony‐stimulating factor‐1 gene in murine osteoblasts. Journal Of Cellular Physiology 1996, 168: 199-208. PMID: 8647916, DOI: 10.1002/(sici)1097-4652(199607)168:1<199::aid-jcp24>3.0.co;2-1.Peer-Reviewed Original ResearchConceptsColony-stimulating factor-1CSF-1 expressionMC3T3-E1 cellsCSF-1 mRNACSF-1 gene transcriptionMRNA stability studiesFactor 1 geneNew protein synthesisSecond messenger pathwaysPrimary mouse osteoblastsColony-stimulating factor-1 (CSF-1) geneTranscriptional mechanismsGene transcriptionResponsive cell linesInduces transcriptionNorthern analysisProgenitor proliferationTNF-alpha treatmentProtein synthesisCAMP pathwayTranscript expressionMessenger pathwaysMurine osteoblastsCycloheximide treatmentCellular mechanisms