1991
Synthesis and evaluation of two new inhibitors of EPSP synthase
Pansegrau P, Anderson K, Widlanski T, Ream J, Sammons R, Sikorski J, Knowles J. Synthesis and evaluation of two new inhibitors of EPSP synthase. Tetrahedron Letters 1991, 32: 2589-2592. DOI: 10.1016/s0040-4039(00)78792-2.Peer-Reviewed Original Research
1988
A tetrahedral intermediate in the EPSP synthase reaction observed by rapid quench kinetics.
Anderson K, Sikorski J, Johnson K. A tetrahedral intermediate in the EPSP synthase reaction observed by rapid quench kinetics. Biochemistry 1988, 27: 7395-406. PMID: 3061457, DOI: 10.1021/bi00419a034.Peer-Reviewed Original ResearchConceptsPhosphoenol pyruvateBurst of product formationPre-steady-state burstQuantitation of reaction productsTransient-state kinetic analysisEnzyme-bound intermediateShikimate 3-phosphateSingle turnover experimentsPre-steady-stateSubstrate trapping experimentsRelease of substratesEquilibrium constantsSynthase reactionExcess enzymeBinding rateAbsence of phosphatePyruvateReverse reactionEnzymeTurnover experimentsEnzymatic reactionsKinetic competenceEnzyme concentrationFormation of productsConcentration of phosphate