1998
Catalytic Mechanism of Kdo8P Synthase: Transient Kinetic Studies and Evaluation of a Putative Reaction Intermediate †
Liang P, Lewis J, Anderson K, Kohen A, D'Souza F, Benenson Y, Baasov T. Catalytic Mechanism of Kdo8P Synthase: Transient Kinetic Studies and Evaluation of a Putative Reaction Intermediate †. Biochemistry 1998, 37: 16390-16399. PMID: 9819231, DOI: 10.1021/bi981374w.Peer-Reviewed Original ResearchConceptsTransient kinetic studiesKDO8P synthaseRapid chemical quench experimentsIntermediate 2Chemical quench experimentsKinetic studiesBeta-pyranose formPutative reaction intermediatesChemical synthesisNMR spectroscopySynthetic 2Anomeric phosphatesReaction intermediatesCatalytic pathwayReaction pathwaysEnzyme catalysisCatalytic mechanismTurnover conditionsMechanistic pathwaysPutative reactionsReactionPhosphate hydrolysisSubstrate activityCorresponding control experimentsAlternate substrates
1997
Catalytic mechanism of KDO8P synthase. Pre-steady-state kinetic analysis using rapid chemical quench flow methods
Liang P, Kohen A, Baasov T, Anderson K. Catalytic mechanism of KDO8P synthase. Pre-steady-state kinetic analysis using rapid chemical quench flow methods. Bioorganic & Medicinal Chemistry Letters 1997, 7: 2463-2468. DOI: 10.1016/s0960-894x(97)10021-x.Peer-Reviewed Original Research