1998
Loop Closure and Intersubunit Communication in Tryptophan Synthase † , ‡
Schneider T, Gerhardt E, Lee M, Liang P, Anderson K, Schlichting I. Loop Closure and Intersubunit Communication in Tryptophan Synthase † , ‡. Biochemistry 1998, 37: 5394-5406. PMID: 9548921, DOI: 10.1021/bi9728957.Peer-Reviewed Original ResearchConceptsBeta-active siteMechanism of allosteric activationAlpha-active siteAlpha subunitTryptophan synthase alpha2beta2 complexPyridoxal phosphateCofactor pyridoxal phosphatePresence of serineSalmonella typhimuriumIntersubunit communicationTryptophan synthaseAllosteric activationAlpha2beta2 complexAllosteric propertiesAlpha-reactionBeta-reactionBeta subunitStructural basisAminoacrylate intermediateAminoacrylatePathwayBindingSitesTryptophanSerine
1996
Intersubunit Communication in Tryptophan Synthase by Carbon-13 and Fluorine-19 REDOR NMR †
McDowell L, Lee M, McKay R, Anderson K, Schaefer J. Intersubunit Communication in Tryptophan Synthase by Carbon-13 and Fluorine-19 REDOR NMR †. Biochemistry 1996, 35: 3328-3334. PMID: 8605170, DOI: 10.1021/bi9518297.Peer-Reviewed Original ResearchConceptsProton dipolar decouplingMagic angle spinningLocal electric field gradientsElectric field gradientIsotropic shiftsLigand bindingChemical shiftsNMR spectraConformational gatingEnzyme tryptophan synthaseBeta subunitCarbon-13Dipolar decouplingTryptophan synthaseMother liquorResolved linesConformational rearrangementsBinding of serineNMRLigandField gradientEnzyme complexIntersubunit communicationTyrosine residuesSubunit