1999
The Catalytic Mechanism of EPSP Synthase Revisited †
Lewis J, Johnson K, Anderson K. The Catalytic Mechanism of EPSP Synthase Revisited †. Biochemistry 1999, 38: 7372-7379. PMID: 10353849, DOI: 10.1021/bi9830258.Peer-Reviewed Original ResearchMeSH Keywords3-Phosphoshikimate 1-CarboxyvinyltransferaseAlkyl and Aryl TransferasesAmino Acid SubstitutionBinding SitesCatalysisChromatography, High Pressure LiquidEscherichia coliFreezingKineticsMutagenesis, Site-DirectedNuclear Magnetic Resonance, BiomolecularPhosphoenolpyruvateProtonsSubstrate SpecificityConceptsEPSP synthaseEnzyme intermediateKinetic competenceSingle-turnover experimentsSubstrate to productSolid-state NMRSolid-state NMR studiesEnzyme assaysEnzyme reaction pathwaySDS-PAGECatalytic mechanismDegrees CSpeciesEnzymeIntermediate speciesNMR studiesSide productsCharacterized reaction productsSample preparationDisappearance of substrateSynthaseReaction productsFormation of productsBreakdown productsReaction pathways
1995
Reevaluating glyphosate as a transition-state inhibitor of EPSP synthase: identification of an EPSP synthase.EPSP.glyphosate ternary complex.
Sammons R, Gruys K, Anderson K, Johnson K, Sikorski J. Reevaluating glyphosate as a transition-state inhibitor of EPSP synthase: identification of an EPSP synthase.EPSP.glyphosate ternary complex. Biochemistry 1995, 34: 6433-40. PMID: 7756274, DOI: 10.1021/bi00019a024.Peer-Reviewed Original ResearchConceptsEPSP synthaseTernary complexShikimate 3-phosphateSteady-state kineticsEnzyme active siteTransition-state analogSubstrate turnoverSynthase reactionTransition-state inhibitorsEnzymeAssociated with PEPUncompetitive inhibitorBinding resultsSynthaseActive siteFluorescence titration experimentsShikimateOxonium ionsTurnoverInteraction of glyphosateTitration experiments
1993
Synthesis & characterization of N-amino-glyphosate as a potent analog inhibitor of E. coli EPSP synthase.
Knowles W, Anderson K, Andrew S, Phillion D, Ream J, Johnson K, Sikorski J. Synthesis & characterization of N-amino-glyphosate as a potent analog inhibitor of E. coli EPSP synthase. Bioorganic & Medicinal Chemistry Letters 1993, 3: 2863-2868. DOI: 10.1016/s0960-894x(01)80780-0.Peer-Reviewed Original Research
1991
Synthesis and evaluation of two new inhibitors of EPSP synthase
Pansegrau P, Anderson K, Widlanski T, Ream J, Sammons R, Sikorski J, Knowles J. Synthesis and evaluation of two new inhibitors of EPSP synthase. Tetrahedron Letters 1991, 32: 2589-2592. DOI: 10.1016/s0040-4039(00)78792-2.Peer-Reviewed Original Research
1990
An Enzyme-Targeted Herbicide Design Program Based on EPSP Synthase: Chemical Mechanism and Glyphosate Inhibition Studies
Sikorski J, Anderson K, Cleary D, Miller M, Pansegrau P, Ream J, Sammons R, Johnson K. An Enzyme-Targeted Herbicide Design Program Based on EPSP Synthase: Chemical Mechanism and Glyphosate Inhibition Studies. Industry-University Cooperative Chemistry Program Symposia 1990, 23-39. DOI: 10.1007/978-1-4757-9637-7_3.Peer-Reviewed Original Research