2018
Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment
Bonello TT, Perez-Vale KZ, Sumigray KD, Peifer M. Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment. Development 2018, 145: dev157941. PMID: 29361565, PMCID: PMC5825837, DOI: 10.1242/dev.157941.Peer-Reviewed Original ResearchMeSH KeywordsAdherens JunctionsAnimalsAnimals, Genetically ModifiedCell PolarityDrosophila melanogasterDrosophila ProteinsFemaleGastrulationGene Knockdown TechniquesGuanine Nucleotide Exchange FactorsIntracellular Signaling Peptides and ProteinsMaleModels, BiologicalProtein Interaction Domains and MotifsProtein TransportRNA InterferenceShelterin ComplexTelomere-Binding ProteinsConceptsApical-basal polarityPolarity establishmentMembrane localizationEpithelial apical-basal polarityAdherens junction positioningBazooka/Par3Adherens junction assemblyMost animal tissuesSmall GTPase Rap1Polarity initiationApical retentionNascent junctionsRA domainJunction assemblyAdherens junctionsTricellular junctionsPolarized cellsGTPase Rap1Macromolecular assembliesApical activationRap1Spatial organizationAnimal tissuesMultiple mechanismsEstablishment model
2016
Abelson kinase acts as a robust, multifunctional scaffold in regulating embryonic morphogenesis
Rogers EM, Spracklen AJ, Bilancia CG, Sumigray KD, Allred SC, Nowotarski SH, Schaefer KN, Ritchie BJ, Peifer M. Abelson kinase acts as a robust, multifunctional scaffold in regulating embryonic morphogenesis. Molecular Biology Of The Cell 2016, 27: 2613-2631. PMID: 27385341, PMCID: PMC4985262, DOI: 10.1091/mbc.e16-05-0292.Peer-Reviewed Original ResearchConceptsF-actin-binding domainCell biological rolesCell shape changesDifferent protein motifsSeries of mutantsTyrosine kinase domainAbl functionActin regulationDrosophila morphogenesisEmbryonic morphogenesisProtein motifsAbelson familyPXXP motifInteraction domainKinase domainAbelson kinaseLinker regionKey regulatorAbl SH3Biological roleCellular behaviorMorphogenesisCell behaviorNormal developmentSH3
2013
Rap1 and Canoe/afadin are essential for establishment of apical–basal polarity in the Drosophila embryo
Choi W, Harris NJ, Sumigray KD, Peifer M. Rap1 and Canoe/afadin are essential for establishment of apical–basal polarity in the Drosophila embryo. Molecular Biology Of The Cell 2013, 24: 945-963. PMID: 23363604, PMCID: PMC3608504, DOI: 10.1091/mbc.e12-10-0736.Peer-Reviewed Original ResearchMeSH KeywordsAdherens JunctionsAnimalsCell LineCell PolarityCell ShapeCytoskeletonDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianGreen Fluorescent ProteinsIntracellular Signaling Peptides and ProteinsMicroscopy, ConfocalModels, BiologicalMutationProtein Kinase CRap1 GTP-Binding ProteinsRNA InterferenceConceptsAtypical protein kinase CCanoe/AfadinPolarity establishmentPolarity cuesDrosophila embryosAdherens junctionsApical-basal cell polarityBazooka/Par3Apical-basal polarityAbsence of Rap1Columnar cell shapeSmall GTPase Rap1Protein kinase CCell polarityBazookaGTPase Rap1Protein networkRap1Cell shapeLinear pathwaySuperb modelKinase COrgan architectureAfadinCytoskeleton