Single-molecule imaging of the BAR-domain protein Pil1p reveals filament-end dynamics
Lacy MM, Baddeley D, Berro J. Single-molecule imaging of the BAR-domain protein Pil1p reveals filament-end dynamics. Molecular Biology Of The Cell 2017, 28: 2251-2259. PMID: 28659415, PMCID: PMC5555653, DOI: 10.1091/mbc.e17-04-0238.Peer-Reviewed Original ResearchConceptsBAR domain proteinsSingle-molecule strategySingle-molecule imagingOligomeric filamentsFission yeastConventional fluorescence microscopyDynamic filamentsCytoplasmic faceMolecular assembliesLocalized recruitmentPlasma membraneEisosomesMacromolecular assembliesProtein dynamicsStable assemblyFluorescence microscopySuch assembliesPhysiological conditionsNanometer-scale featuresFirst direct observationProteinAssemblyHeterogeneous dynamicsPhotobleachingFilaments