2017
High-speed super-resolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches
Arasada R, Sayyad WA, Berro J, Pollard TD. High-speed super-resolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches. Molecular Biology Of The Cell 2017, 29: mbc.e17-06-0415. PMID: 29212877, PMCID: PMC5996959, DOI: 10.1091/mbc.e17-06-0415.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActin-Related Protein 2-3 ComplexActinsAdaptor Proteins, Vesicular TransportCell MembraneClathrinClathrin-Coated VesiclesCytokinesisCytoplasmEndocytosisMicrofilament ProteinsMicroscopy, FluorescenceMyosin Type IProtein BindingSchizosaccharomycesSchizosaccharomyces pombe ProteinsConceptsActin filamentsLive fission yeast cellsEndocytic actin patchesSites of endocytosisFission yeast cellsNucleation-promoting factorsActin binding proteinsActin filament polymerizationCell surface receptorsActin patchesVesicle scissionFission yeastYeast clathrinArp2/3 complexSuperresolution localization microscopyTubule elongationFilament polymerizationRegulated mannerRegulatory proteinsMolecular mechanismsYeast cellsBinding proteinClathrinIndependent pathwaysCell surface
2010
Mathematical Modeling of Endocytic Actin Patch Kinetics in Fission Yeast: Disassembly Requires Release of Actin Filament Fragments
Berro J, Sirotkin V, Pollard TD. Mathematical Modeling of Endocytic Actin Patch Kinetics in Fission Yeast: Disassembly Requires Release of Actin Filament Fragments. Molecular Biology Of The Cell 2010, 21: 2905-2915. PMID: 20587776, PMCID: PMC2921120, DOI: 10.1091/mbc.e10-06-0494.Peer-Reviewed Original ResearchActin CytoskeletonActin Depolymerizing FactorsActin-Related Protein 2-3 ComplexActinsAdenosine TriphosphateAlgorithmsComputer SimulationEndocytosisHydrolysisImmunoblottingKineticsModels, BiologicalPolymersProtein BindingSchizosaccharomycesSchizosaccharomyces pombe ProteinsWiskott-Aldrich Syndrome ProteinQuantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast
Sirotkin V, Berro J, Macmillan K, Zhao L, Pollard TD. Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast. Molecular Biology Of The Cell 2010, 21: 2894-2904. PMID: 20587778, PMCID: PMC2921122, DOI: 10.1091/mbc.e10-02-0157.Peer-Reviewed Original ResearchMeSH KeywordsActin-Related Protein 2-3 ComplexActinsClathrinEndocytosisLuminescent ProteinsMicrofilament ProteinsMicroscopy, FluorescenceModels, BiologicalProtein BindingSchizosaccharomycesSchizosaccharomyces pombe ProteinsTime FactorsConceptsFission yeastActin assemblyActin patch assemblyEndocytic actin patchesEndocytic adaptor proteinActivators of Arp2/3Plasma membrane invaginationsRecruitment of dynaminQuantitative confocal microscopyActin filament fragmentationActin patchesArp2/3 complexPatch assemblyAdaptor proteinMembrane invaginationsCross-linked filamentsFluorescent proteinClathrin moleculesFilament fragmentationProteinConfocal microscopyYeastBranched networkIndividual patchesAssembly