2008
TNF-&agr; Induces MMP2 Gelatinase Activity and MT1-MMP Expression in an In Vitro Model of Nucleus Pulposus Tissue Degeneration
Séguin CA, Pilliar RM, Madri JA, Kandel RA. TNF-&agr; Induces MMP2 Gelatinase Activity and MT1-MMP Expression in an In Vitro Model of Nucleus Pulposus Tissue Degeneration. Spine 2008, 33: 356-365. PMID: 18277865, DOI: 10.1097/brs.0b013e3181642a5e.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleElectrophoretic Mobility Shift AssayExtracellular Signal-Regulated MAP KinasesGene ExpressionImmunoblottingIn Vitro TechniquesIntervertebral DiscLuciferasesMatrix Metalloproteinase 14Matrix Metalloproteinase 2Reverse Transcriptase Polymerase Chain ReactionTumor Necrosis Factor-alphaUp-RegulationConceptsMMP-2 gelatinase activityERK MAPK pathwayTranscriptional activationMT1-MMPElectrophoretic mobility shift assaysMT1-MMP promoterMMP-2 geneMobility shift assaysSignal transduction mechanismsProtein levelsERK 1/2 activationNP tissuesTranscription factorsShift assaysMT1-MMP expressionReporter constructsTNF-alpha inductionERK MAPKGelatinase activityLuciferase constructEgr-1TNF-alpha treatmentMMP-2 activationSP-1Transduction mechanisms
1999
Extracellular Matrix-Driven Matrix Metalloproteinase Production in Endothelial Cells Implications for Angiogenesis
Haas T, Madri J. Extracellular Matrix-Driven Matrix Metalloproteinase Production in Endothelial Cells Implications for Angiogenesis. Trends In Cardiovascular Medicine 1999, 9: 70-77. PMID: 10578520, DOI: 10.1016/s1050-1738(99)00014-6.Peer-Reviewed Original ResearchConceptsInterstitial matrix moleculesCell-extracellular matrix interactionsBasement membrane matrixMatrix metalloproteinasesTranscriptional activationBlood vessel growthMatrix interactionsNew blood vessel growthMatrix moleculesCell surfaceExtracellular matrixMT1-MMPAdjacent cellsCell interactionsMatrix metalloproteinase productionCurrent understandingVessel growthParticular matrix metalloproteinasesEndothelial cell interactionsPotential roleAngiogenesisMetalloproteinase productionBasement membraneMMP-2Signaling