2023
Identification and validation of urinary CXCL9 as a biomarker for diagnosis of acute interstitial nephritis
Moledina D, Obeid W, Smith R, Rosales I, Sise M, Moeckel G, Kashgarian M, Kuperman M, Campbell K, Lefferts S, Meliambro K, Bitzer M, Perazella M, Luciano R, Pober J, Cantley L, Colvin R, Wilson F, Parikh C. Identification and validation of urinary CXCL9 as a biomarker for diagnosis of acute interstitial nephritis. Journal Of Clinical Investigation 2023, 133: e168950. PMID: 37395276, PMCID: PMC10313360, DOI: 10.1172/jci168950.Peer-Reviewed Original ResearchConceptsUrinary CXCL9External validation cohortValidation cohortControl groupAIN diagnosisDiscovery cohortKidney tissueDiagnostic biomarkersAcute interstitial nephritisCXCL9 mRNA expressionAcute kidney injuryBiopsy-confirmed diagnosisAvailable clinical testsNational InstituteKidney injuryTubulointerstitial nephritisInterstitial nephritisKidney biopsyHistological confirmationHistological diagnosisTreatment optionsLymphocyte chemotaxisCXCL9MRNA expression differencesPatients
2009
TNF Receptors Differentially Signal and Are Differentially Expressed and Regulated in the Human Heart
Al-Lamki R, Brookes AP, Wang J, Reid MJ, Parameshwar J, Goddard MJ, Tellides G, Wan T, Min W, Pober JS, Bradley JR. TNF Receptors Differentially Signal and Are Differentially Expressed and Regulated in the Human Heart. American Journal Of Transplantation 2009, 9: 2679-2696. PMID: 19788501, PMCID: PMC3517885, DOI: 10.1111/j.1600-6143.2009.02831.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell CycleCell DeathEndothelium, VascularEnzyme ActivationGraft RejectionHeart TransplantationHumansMAP Kinase Kinase Kinase 5MiceMice, KnockoutMyocardiumMyocytes, CardiacOrgan Culture TechniquesProtein-Tyrosine KinasesReceptors, Tumor Necrosis Factor, Type IReceptors, Tumor Necrosis Factor, Type IIRNA, MessengerTumor Necrosis Factor-alphaConceptsVascular endothelial cellsCardiac allograftsCell cycle entryApoptosis signal-regulating kinase 1Cycle entryExpression of TNFTNFR1 knockoutNecrosis factorTarget cell responseTNFTNF receptorCardiac fibroblastsCell responsesSignal-regulated kinases 1TNF responseASK1 activationMyocardiumEndothelial cellsEpithelial tyrosine kinaseTNFR2Human heartOrgan cultureTNFR1Etk activationAllografts
2003
Combining altered levels of effector transcripts in circulating T cells with a marker of endothelial injury is specific for active graft-versus-host disease
Biedermann BC, Tsakiris DA, Gregor M, Pober JS, Gratwohl A. Combining altered levels of effector transcripts in circulating T cells with a marker of endothelial injury is specific for active graft-versus-host disease. Bone Marrow Transplantation 2003, 32: 1077-1084. PMID: 14625579, DOI: 10.1038/sj.bmt.1704258.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCase-Control StudiesEndothelium, VascularFas Ligand ProteinGraft vs Host DiseaseHematopoietic Stem Cell TransplantationInterferon-gammaLymphocyte ActivationMembrane GlycoproteinsMolecular Diagnostic TechniquesRNA, MessengerSensitivity and SpecificitySurvivorsT-Lymphocytes, CytotoxicTransplantation, HomologousVon Willebrand FactorConceptsCytotoxic T lymphocytesEndothelial injuryT cell activationT cellsT lymphocytesHost diseaseSCT recipientsAllogeneic stem cell transplantationAllospecific cytotoxic T lymphocytesIFN-γ transcript levelsActive chronic GVHDEndothelial injury resultsCD8 T lymphocytesCD8 T cellsCD4 T cellsLong-term survivorsStem cell transplantationImportant effector cellsVon Willebrand factor plasma levelsAge-matched controlsVascular endothelial cellsChronic GVHDActive graftEffector cellsCell transplantation
2001
Suppression of Vascular Endothelial Growth Factor-Mediated Endothelial Cell Protection by Survivin Targeting
Mesri M, Morales-Ruiz M, Ackermann E, Bennett C, Pober J, Sessa W, Altieri D. Suppression of Vascular Endothelial Growth Factor-Mediated Endothelial Cell Protection by Survivin Targeting. American Journal Of Pathology 2001, 158: 1757-1765. PMID: 11337373, PMCID: PMC1891951, DOI: 10.1016/s0002-9440(10)64131-4.Peer-Reviewed Original ResearchApoptosisCell MovementCells, CulturedDNADNA, AntisenseDose-Response Relationship, DrugEndothelial Growth FactorsEndothelium, VascularGene Expression RegulationHumansInhibitor of Apoptosis ProteinsLymphokinesMicrotubule-Associated ProteinsNeoplasm ProteinsProteinsRNA, MessengerSurvivinVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsInterleukin-11 Up-Regulates Survivin Expression in Endothelial Cells through a Signal Transducer and Activator of Transcription-3 Pathway
Mahboubi K, Li F, Plescia J, Kirkiles-Smith N, Mesri M, Du Y, Carroll J, Elias J, Altieri D, Pober J. Interleukin-11 Up-Regulates Survivin Expression in Endothelial Cells through a Signal Transducer and Activator of Transcription-3 Pathway. Laboratory Investigation 2001, 81: 327-334. PMID: 11310826, DOI: 10.1038/labinvest.3780241.Peer-Reviewed Original ResearchMeSH KeywordsDNA-Binding ProteinsEndothelium, VascularGene ExpressionHumansInhibitor of Apoptosis ProteinsInterleukin-11Microtubule-Associated ProteinsNeoplasm ProteinsPhosphorylationProteinsRNA, MessengerSerineSignal TransductionSTAT1 Transcription FactorSTAT3 Transcription FactorSurvivinTrans-ActivatorsTranscription, GeneticTransgenesUmbilical VeinsConceptsSignal transducerProtein kinase B/AktDominant-negative STAT3 mutantActivator of transcriptionSurvivin mRNA expressionTranscription 3 (STAT3) pathwayIL-11Survivin protein expressionSTAT3 mutantStimulation of serumProtein expression levelsSurvivin inductionSurvivin expressionAntiapoptotic proteinsInduces expressionMRNA expressionExpression levelsSurvivin proteinProtein expressionCritical mediatorMaximal inductionExpressionSurvivin mRNAProteinDose-dependent manner
1999
Human endothelial cells augment early CD40 ligand expression in activated CD4+ T cells through LFA-3-mediated stabilization of mRNA.
Murakami K, Ma W, Fuleihan R, Pober J. Human endothelial cells augment early CD40 ligand expression in activated CD4+ T cells through LFA-3-mediated stabilization of mRNA. The Journal Of Immunology 1999, 163: 2667-73. PMID: 10453007, DOI: 10.4049/jimmunol.163.5.2667.Peer-Reviewed Original ResearchConceptsCD40 ligand expressionT cellsCD40L mRNALFA-3CD40L expressionICAM-1Ligand expressionICAM-1 antisense oligonucleotideT-cell mRNA expressionCD40L protein expressionPretreatment of ECPromoter-reporter geneHuman endothelial cellsIL-2CD4Capacity of ECMRNA expressionICAM-2Endothelial cellsProtein expressionCD40LAntisense oligonucleotidePHAMRNACells
1998
Interferon Induction of TAP1
Min W, Pober J, Johnson D. Interferon Induction of TAP1. Circulation Research 1998, 83: 815-823. PMID: 9776728, DOI: 10.1161/01.res.83.8.815.Peer-Reviewed Original ResearchMeSH KeywordsATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersDimerizationDNA-Binding ProteinsEndotheliumGene Expression Regulation, EnzymologicGenes, ReporterHeLa CellsHumansInterferon-alphaInterferon-betaInterferon-gammaInterferon-Stimulated Gene Factor 3Interferon-Stimulated Gene Factor 3, gamma SubunitIntracellular Signaling Peptides and ProteinsJanus Kinase 1Janus Kinase 2Major Histocompatibility ComplexPromoter Regions, GeneticProtein Tyrosine Phosphatase, Non-Receptor Type 11Protein Tyrosine Phosphatase, Non-Receptor Type 6Protein Tyrosine PhosphatasesProtein-Tyrosine KinasesProto-Oncogene ProteinsRNA, MessengerSH2 Domain-Containing Protein Tyrosine PhosphatasesSignal TransductionSrc Homology DomainsSTAT1 Transcription FactorSTAT2 Transcription FactorTrans-ActivatorsTranscription Factors
1997
Interferon-γ Rapidly Increases Peptide Transporter (TAP) Subunit Expression and Peptide Transport Capacity in Endothelial Cells*
Ma W, Pober J, Johnson D, Lehner P, Cresswell P. Interferon-γ Rapidly Increases Peptide Transporter (TAP) Subunit Expression and Peptide Transport Capacity in Endothelial Cells*. Journal Of Biological Chemistry 1997, 272: 16585-16590. PMID: 9195970, DOI: 10.1074/jbc.272.26.16585.Peer-Reviewed Original ResearchFunctional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: Implications for CD40–CD40 ligand signaling in atherosclerosis
Mach F, Schönbeck U, Sukhova G, Bourcier T, Bonnefoy J, Pober J, Libby P. Functional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: Implications for CD40–CD40 ligand signaling in atherosclerosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 1931-1936. PMID: 9050882, PMCID: PMC20020, DOI: 10.1073/pnas.94.5.1931.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, T-LymphocyteArteriosclerosisB-LymphocytesBlotting, WesternCD40 AntigensCD40 LigandCells, CulturedEndothelium, VascularFlow CytometryGene Expression RegulationHumansImmunohistochemistryInterferon-gammaInterleukin-1MacrophagesMembrane GlycoproteinsMuscle, Smooth, VascularRNA, MessengerSignal TransductionTumor Necrosis Factor-alphaConceptsHuman vascular endothelial cellsSmooth muscle cellsVascular endothelial cellsHuman atherosclerotic lesionsHuman macrophagesCell typesEndothelial cellsMuscle cellsHuman vascular smooth muscle cellsVascular smooth muscle cellsDe novo synthesisCD40 ligandBroad functionsAtherosclerotic lesionsCD40 SignalingTumor necrosis factor alphaFunctional CD40 ligandInvolvement of inflammationCultured human vascular endothelial cellsCD40-CD40 ligandNovo synthesisNecrosis factor alphaParacrine activationNormal arterial tissueNovel sourceMechanism of sustained E-selectin expression in cultured human dermal microvascular endothelial cells.
Kluger MS, Johnson DR, Pober JS. Mechanism of sustained E-selectin expression in cultured human dermal microvascular endothelial cells. The Journal Of Immunology 1997, 158: 887-96. PMID: 8993008, DOI: 10.4049/jimmunol.158.2.887.Peer-Reviewed Original Research
1996
IL-4 and IL-13 activate the JAK2 tyrosine kinase and Stat6 in cultured human vascular endothelial cells through a common pathway that does not involve the gamma c chain.
Palmer-Crocker RL, Hughes CC, Pober JS. IL-4 and IL-13 activate the JAK2 tyrosine kinase and Stat6 in cultured human vascular endothelial cells through a common pathway that does not involve the gamma c chain. Journal Of Clinical Investigation 1996, 98: 604-609. PMID: 8698849, PMCID: PMC507467, DOI: 10.1172/jci118829.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCells, CulturedEndothelium, VascularHumansInterleukin-3Interleukin-4Janus Kinase 2Molecular Sequence DataPhosphorylationProtein-Tyrosine KinasesProto-Oncogene ProteinsReceptors, Interleukin-2RNA, MessengerSTAT6 Transcription FactorTrans-ActivatorsTyrosineVascular Cell Adhesion Molecule-1ConceptsIL-4 responsesIL-13IL-4Human endothelial cellsEndothelial cellsVascular cell adhesion molecule-1Cell adhesion molecule-1Cultured human vascular endothelial cellsAdhesion molecule-1IL-15 receptorTyrosine kinaseCommon signaling subunitHuman vascular endothelial cellsJAK2 tyrosine kinaseVascular endothelial cellsSTAT6 transcription factorReverse transcription-PCR methodGamma c chainTranscription-PCR methodGC-independent pathwayIL-2IL-9IL-7IL-4RMolecule-1Activation and homologous desensitization of human endothelial cells by CD40 ligand, tumor necrosis factor, and interleukin 1.
Karmann K, Min W, Fanslow WC, Pober JS. Activation and homologous desensitization of human endothelial cells by CD40 ligand, tumor necrosis factor, and interleukin 1. Journal Of Experimental Medicine 1996, 184: 173-182. PMID: 8691131, PMCID: PMC2192678, DOI: 10.1084/jem.184.1.173.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCalcium-Calmodulin-Dependent Protein KinasesCD40 LigandCell Adhesion MoleculesE-SelectinEndothelium, VascularGene ExpressionHumansIntercellular Adhesion Molecule-1Interleukin-1JNK Mitogen-Activated Protein KinasesMembrane GlycoproteinsMitogen-Activated Protein KinasesMolecular Sequence DataOligodeoxyribonucleotidesRNA, MessengerTranscription, GeneticTumor Necrosis Factor-alphaVascular Cell Adhesion Molecule-1ConceptsVascular cell adhesion molecule-1Intercellular adhesion molecule-1Tumor necrosis factorHuman umbilical vein endothelial cellsAdhesion molecule-1CD40 ligandHomologous desensitizationIL-1E-selectinJun NH2-terminal kinaseNecrosis factorInterleukin-1Molecule-1ATF-2/c-JunEndothelial cellsCell adhesion molecule-1Kinetically coordinated induction of TAP1 and HLA class I by IFN-gamma: the rapid induction of TAP1 by IFN-gamma is mediated by Stat1 alpha.
Min W, Pober JS, Johnson DR. Kinetically coordinated induction of TAP1 and HLA class I by IFN-gamma: the rapid induction of TAP1 by IFN-gamma is mediated by Stat1 alpha. The Journal Of Immunology 1996, 156: 3174-83. PMID: 8617938, DOI: 10.4049/jimmunol.156.9.3174.Peer-Reviewed Original ResearchAllelesATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersBase SequenceCysteine EndopeptidasesDNA-Binding ProteinsEndopeptidasesExtracellular Matrix ProteinsGene Expression RegulationHeLa CellsHistocompatibility Antigens Class IHLA AntigensHLA-B AntigensHumansInterferon Regulatory Factor-1Interferon-gammaInterferon-Stimulated Gene Factor 3KineticsMacromolecular SubstancesMolecular Sequence DataNerve Tissue ProteinsNF-kappa BPhosphoproteinsPromoter Regions, GeneticRNA, MessengerTranscription FactorsTranscription, Genetic
1995
IL-8 and angiogenesis: evidence that human endothelial cells lack receptors and do not respond to IL-8 in vitro
Petzelbauer P, Watson C, Watson C, Pfau S, Pober J. IL-8 and angiogenesis: evidence that human endothelial cells lack receptors and do not respond to IL-8 in vitro. Cytokine 1995, 7: 267-272. PMID: 7543779, DOI: 10.1006/cyto.1995.0031.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsIL-8Cultured human umbilical vein endothelial cellsDermal microvascular endothelial cellsEndothelial cellsHuman endothelial cellsRecombinant human IL-8IL-8 treatmentMicrovascular human endothelial cellsReceptor mRNA expressionEndothelial cell growth factorHuman IL-8Human dermal microvascular endothelial cellsMicrovascular endothelial cellsCytoplasmic calcium concentrationUmbilical vein endothelial cellsCell growth factorVein endothelial cellsAngiogenic factorsMRNA expressionDirect actionGrowth factorCalcium concentrationIndirect actionProliferative rate
1993
Costimulation of peripheral blood T cell activation by human endothelial cells. Enhanced IL-2 transcription correlates with increased c-fos synthesis and increased Fos content of AP-1.
Hughes CC, Pober JS. Costimulation of peripheral blood T cell activation by human endothelial cells. Enhanced IL-2 transcription correlates with increased c-fos synthesis and increased Fos content of AP-1. The Journal Of Immunology 1993, 150: 3148-60. PMID: 8468462, DOI: 10.4049/jimmunol.150.8.3148.Peer-Reviewed Original ResearchConceptsC-fos mRNAIL-2 transcriptionNuclear factorT cellsTranscription factor AP-1PHA activationPeripheral blood T cell activationAP-1Blood T cell activationFactor AP-1C-fosC-JunIL-2 productionHuman umbilical vein ECT cell activationIL-2 mRNAC-Fos proteinEC costimulationEndothelial cells (EC) actAllograft rejectionHuman endothelial cellsIL-2 regulationCostimulatory signalsCell activationKey molecular mechanisms
1992
Cytokines increase transporter in antigen processing-1 expression more rapidly than HLA class I expression in endothelial cells.
Epperson DE, Arnold D, Spies T, Cresswell P, Pober JS, Johnson DR. Cytokines increase transporter in antigen processing-1 expression more rapidly than HLA class I expression in endothelial cells. The Journal Of Immunology 1992, 149: 3297-301. PMID: 1385520, DOI: 10.4049/jimmunol.149.10.3297.Peer-Reviewed Original Research
1991
Interleukin-4 induces the synthesis and secretion of MCP-1/JE by human endothelial cells.
Rollins BJ, Pober JS. Interleukin-4 induces the synthesis and secretion of MCP-1/JE by human endothelial cells. American Journal Of Pathology 1991, 138: 1315-9. PMID: 2053591, PMCID: PMC1886397.Peer-Reviewed Original ResearchConceptsMCP-1/JEIL-4Interleukin-1Endothelial cellsHuman endothelial cellsJE proteinInterleukin 4 inducesTreatment of ECMCP-1/JE mRNATumor necrosis factorMonocyte-specific chemoattractantAnti-tumor propertiesConcomitant treatmentNecrosis factorInterleukin-4SecretionMore secretionJE mRNAInduced expressionTreatmentTumor necrosis factor induction of endothelial cell surface antigens is independent of protein kinase C activation or inactivation. Studies with phorbol myristate acetate and staurosporine.
Ritchie AJ, Johnson DR, Ewenstein BM, Pober JS. Tumor necrosis factor induction of endothelial cell surface antigens is independent of protein kinase C activation or inactivation. Studies with phorbol myristate acetate and staurosporine. The Journal Of Immunology 1991, 146: 3056-62. PMID: 1707932, DOI: 10.4049/jimmunol.146.9.3056.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAlkaloidsCell Adhesion MoleculesCell CompartmentationE-SelectinEndothelium, VascularEnzyme ActivationHistocompatibility Antigens Class IHumansIn Vitro TechniquesIntercellular Adhesion Molecule-1IsoquinolinesPhosphoproteinsPiperazinesProtein BiosynthesisProtein Kinase CRNA, MessengerSignal TransductionStaurosporineTetradecanoylphorbol AcetateTumor Necrosis Factor-alphaConceptsAdhesion molecule-1Intercellular adhesion molecule-1Molecule-1Endothelial leukocyte adhesion molecule-1 expressionProtein kinase CAdhesion molecule-1 expressionEndothelial leukocyte adhesion molecule-1Leukocyte adhesion molecule-1Surface Ag expressionMolecule-1 expressionClass I MHC moleculesClass I MHCEndothelial cell surface antigensAction of TNFI MHC moleculesCell surface antigensNecrosis factor inductionMechanism of actionPhorbol myristate acetatePKC activationTNF effectsI MHCSurface antigenProtein kinase C activationActivation of PKCWhat can be learned from the expression of endothelial adhesion molecules in tissues?
Pober J, Cotran RS. What can be learned from the expression of endothelial adhesion molecules in tissues? Laboratory Investigation 1991, 64: 301-5. PMID: 2002650.Peer-Reviewed Original Research
1990
IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1.
Doukas J, Pober JS. IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1. The Journal Of Immunology 1990, 145: 1727-33. PMID: 1697308, DOI: 10.4049/jimmunol.145.6.1727.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, NorthernCell Adhesion MoleculesDrug SynergismEndothelium, VascularE-SelectinFluorescent Antibody TechniqueGene ExpressionHistocompatibility Antigens Class IHumansIn Vitro TechniquesIntercellular Adhesion Molecule-1Interferon-gammaInterleukin-1Recombinant ProteinsRNA, MessengerTumor Necrosis Factor-alphaConceptsEndothelial leukocyte adhesion molecule-1ELAM-1 expressionIL-1 betaIFN-gammaIL-1Endothelial cellsEC activationIL-1 beta responseIntercellular adhesion molecule-1 expressionAdhesion molecule-1 expressionMHC Ag expressionLeukocyte adhesion molecule-1Treatment of ECICAM-1 expressionMolecule-1 expressionClass I MHC moleculesTumor necrosis factorAdhesion molecule-1ICAM-1 mRNAClass I MHCI MHC moleculesIFN-gamma effectELAM-1 mRNACultured endothelial cellsIncrease TNF